##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632632.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21363
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.014838739877355	34.0	33.0	34.0	31.0	34.0
2	33.16093245330712	34.0	33.0	34.0	31.0	34.0
3	33.226747179703224	34.0	34.0	34.0	31.0	34.0
4	36.49468707578524	37.0	37.0	37.0	35.0	37.0
5	36.478865327903385	37.0	37.0	37.0	35.0	37.0
6	36.529326405467394	37.0	37.0	37.0	35.0	37.0
7	36.51593877264429	37.0	37.0	37.0	35.0	37.0
8	36.483546318400975	37.0	37.0	37.0	35.0	37.0
9	38.30042597013528	39.0	39.0	39.0	37.0	39.0
10	38.33230351542387	39.0	39.0	39.0	37.0	39.0
11	38.40289285212751	39.0	39.0	39.0	37.0	39.0
12	38.33445677105276	39.0	39.0	39.0	37.0	39.0
13	38.3629171932781	39.0	39.0	39.0	37.0	39.0
14	39.90820577634227	41.0	40.0	41.0	38.0	41.0
15	39.909141974441795	41.0	40.0	41.0	38.0	41.0
16	39.8885456162524	41.0	40.0	41.0	38.0	41.0
17	39.88774984786781	41.0	40.0	41.0	38.0	41.0
18	39.861442681271356	41.0	40.0	41.0	38.0	41.0
19	39.873191967420304	41.0	40.0	41.0	38.0	41.0
20	39.83288863923606	41.0	40.0	41.0	38.0	41.0
21	39.82099892337219	41.0	40.0	41.0	38.0	41.0
22	39.814726396105414	41.0	40.0	41.0	38.0	41.0
23	39.767120722744934	41.0	40.0	41.0	38.0	41.0
24	39.756073585170626	41.0	40.0	41.0	38.0	41.0
25	39.7131957122127	41.0	40.0	41.0	38.0	41.0
26	39.63385292327857	41.0	40.0	41.0	37.0	41.0
27	39.510742873191965	41.0	40.0	41.0	37.0	41.0
28	39.471001263867436	41.0	39.0	41.0	37.0	41.0
29	39.45255816130693	41.0	39.0	41.0	37.0	41.0
30	39.41918269905912	41.0	39.0	41.0	36.0	41.0
31	39.33871647240556	41.0	39.0	41.0	36.0	41.0
32	39.30037916023031	41.0	39.0	41.0	36.0	41.0
33	39.204512474839674	41.0	39.0	41.0	35.0	41.0
34	39.18461826522492	41.0	39.0	41.0	35.0	41.0
35	39.13111454383748	41.0	39.0	41.0	35.0	41.0
36	39.07648738473061	41.0	39.0	41.0	35.0	41.0
37	39.02289004353321	41.0	39.0	41.0	35.0	41.0
38	38.90904835463184	40.0	39.0	41.0	35.0	41.0
39	38.86256611899078	40.0	39.0	41.0	35.0	41.0
40	38.78275523100688	40.0	38.0	41.0	35.0	41.0
41	38.71591068670131	40.0	38.0	41.0	35.0	41.0
42	38.63993821092543	40.0	38.0	41.0	35.0	41.0
43	37.978607873426014	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	2.0
21	3.0
22	6.0
23	8.0
24	17.0
25	22.0
26	27.0
27	39.0
28	49.0
29	72.0
30	99.0
31	112.0
32	199.0
33	231.0
34	351.0
35	548.0
36	835.0
37	1642.0
38	3978.0
39	13121.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.10256050180218	16.03707344474091	12.498244628563404	28.362121424893505
2	21.560642231896267	19.266956888077516	31.526471001263868	27.64592987876235
3	21.75724383279502	19.763141880821983	28.371483405888686	30.10813088049431
4	15.985582549267424	15.007255535271263	33.21162758039601	35.7955343350653
5	17.66605813790198	32.94013013153583	31.994570051022798	17.39924167953939
6	36.09043673641342	32.649908720685296	14.258297055656977	17.001357487244302
7	30.697935683190565	27.070168047558862	19.346533726536535	22.885362542714038
8	27.805083555680383	30.852408369611013	19.32312877404859	22.01937930066002
9	28.06721902354538	12.137808360249029	17.652015166409214	42.14295744979638
10	19.63207414688948	23.2083508870477	29.546412020783595	27.613162945279225
11	39.685437438562	19.407386603005197	18.218415016617516	22.68876094181529
12	23.339418620980197	23.311332677994663	25.993540233113325	27.35570846791181
13	35.21041052286664	17.3524317745635	20.31081776903993	27.126339933529938
14	24.359874549454666	19.37930066001966	22.445349435940646	33.815475354585026
15	29.284276552918598	24.26625473950288	19.44483452698591	27.004634180592614
16	27.074849038056453	23.301970696999486	21.883630576229933	27.73954968871413
17	27.205916771988953	23.11473107709591	21.747881851799843	27.93147029911529
18	27.004634180592614	21.640219070355286	23.049197210129662	28.305949538922437
19	28.507232130318776	22.00065533866966	22.791742732762252	26.70036979824931
20	29.387258343865565	21.017647334175912	22.07555118663109	27.519543135327435
21	28.324673500912795	21.69170996582877	21.48574638393484	28.497870149323596
22	28.79745354116931	22.510883302906894	21.050414267659036	27.64124888826476
23	28.975331180077706	21.864906614239572	21.396807564480643	27.762954641202082
24	28.39956934887422	22.21129991106118	21.537237279408323	27.851893460656274
25	28.380845386883863	21.537237279408323	22.459392407433413	27.6225249262744
26	28.745962645695826	22.328324673500912	22.01937930066002	26.906333380143238
27	28.07190001404297	22.112999110611806	22.824509666245376	26.990591209099847
28	26.868885456162523	22.763656789776718	21.74320086130225	28.62425689275851
29	27.55231006881056	22.693441932312876	22.06150821513832	27.69273978373824
30	27.48677620184431	22.496840331414127	23.119412067593505	26.89697139914806
31	28.006366147076722	21.986612367176896	22.300238730515375	27.706782755231007
32	26.002902214108502	21.892992557225107	22.576417169873146	29.527688058793238
33	25.834386556195295	22.01001731966484	24.30370266348359	27.851893460656274
34	26.747179703225203	21.340635678509575	23.821560642231894	28.09062397603333
35	26.414829377896364	22.53896924589243	23.957309366661985	27.08889200954922
36	26.55994008332163	21.80405373777091	24.163272948555914	27.472733230351544
37	27.243364695969667	20.49337639844591	24.163272948555914	28.099985957028505
38	25.609699012311005	21.261058840050552	25.4365023639002	27.69273978373824
39	25.10415203857136	19.880166643261717	26.227589757992792	28.788091560174134
40	25.02925619060993	20.301455788044752	27.95487525160324	26.71441276974208
41	23.634321022328326	19.9737864532135	28.418293310864577	27.973599213593598
42	22.248747835041897	20.96615643870243	29.574497963769137	27.210597762486543
43	21.396807564480643	20.975518419697607	29.349810419884847	28.277863595936903
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.5
4	2.0
5	2.0
6	5.0
7	8.0
8	4.5
9	1.0
10	2.5
11	4.0
12	4.0
13	5.5
14	7.0
15	17.0
16	27.0
17	27.0
18	27.0
19	27.0
20	23.5
21	20.0
22	27.5
23	35.0
24	36.5
25	38.0
26	38.0
27	49.5
28	61.0
29	93.0
30	125.0
31	140.5
32	156.0
33	156.0
34	180.0
35	204.0
36	255.0
37	306.0
38	382.0
39	458.0
40	458.0
41	566.0
42	674.0
43	782.0
44	890.0
45	1116.5
46	1343.0
47	1343.0
48	1518.5
49	1694.0
50	1924.5
51	2155.0
52	2270.0
53	2385.0
54	2385.0
55	2283.5
56	2182.0
57	2145.5
58	2109.0
59	1957.5
60	1806.0
61	1806.0
62	1615.0
63	1424.0
64	1207.5
65	991.0
66	866.5
67	742.0
68	742.0
69	631.5
70	521.0
71	445.5
72	370.0
73	300.5
74	231.0
75	231.0
76	194.0
77	157.0
78	120.0
79	83.0
80	70.5
81	58.0
82	58.0
83	42.5
84	27.0
85	24.0
86	21.0
87	15.5
88	10.0
89	10.0
90	8.0
91	6.0
92	4.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	21363.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.34573795815194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.62179602079226	70.21485746383935
2	5.532652207683576	8.669194401535364
3	1.9896038716615883	4.676309507091701
4	0.9260918922148533	2.9022141085053597
5	0.5078568441178228	1.9894209614754483
6	0.2927645336679214	1.3762112062912513
7	0.3106888928720798	1.7038805411225018
8	0.1433948736332676	0.8987501755371438
9	0.11352094162633684	0.8004493750877686
>10	0.5616299217302981	6.768712259514113
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	45	0.21064457239151807	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	45	0.21064457239151807	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	42	0.19660160089875017	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	33	0.15447268642044656	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	31	0.145110705425268	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	27	0.12638674343491083	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	25	0.11702476243973224	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	25	0.11702476243973224	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	24	0.11234377194214297	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	23	0.10766278144455367	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	22	0.10298179094696439	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0046809904975892895	0.0
24	0.0	0.0	0.0	0.009361980995178579	0.0
25	0.0	0.0	0.0	0.01404297149276787	0.0
26	0.0	0.0	0.0	0.01404297149276787	0.0
27	0.0	0.0	0.0	0.04212891447830361	0.0
28	0.0	0.0	0.0	0.145110705425268	0.0
29	0.0	0.0	0.0	0.33703131582642887	0.0
30	0.0	0.0	0.0	0.580442821701072	0.0
31	0.0	0.0	0.0	1.0813088049431259	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25	1.1935207E-4	37.0	1
GTATCAA	40	0.0018701405	23.125	2
CTCTTAT	70	1.149675E-4	18.5	37
TCTCTTA	115	2.0162122E-5	14.47826	37
GTCTCTT	150	2.4311904E-4	11.1	36
TGTCTCT	190	0.0021355783	8.763158	35
>>END_MODULE