FastQCFastQC Report
Fri 10 Feb 2017
ERR1632631.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632631.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20055
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA480.23934181002243832No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA450.22438294689603588No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT370.18449264522562953No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA330.16454749439042632No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA300.14958863126402394No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC290.14460234355522314No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA280.13961605584642234No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG260.12964348042882073No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC260.12964348042882073No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG250.12465719272001993No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC250.12465719272001993No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT240.11967090501121916TruSeq Adapter, Index 1 (95% over 22bp)
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC230.11468461730241836No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT230.11468461730241836No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA230.11468461730241836No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC220.10969832959361754No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT220.10969832959361754No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC220.10969832959361754No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA220.10969832959361754No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT210.10471204188481677No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC210.10471204188481677No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG210.10471204188481677No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA210.10471204188481677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC200.001799145437.037
GTCTCTT1000.001725827512.95000137
TGTCTCT1653.3060132E-612.33333437
CTGTCTC1851.18481E-511.036
Produced by FastQC (version 0.11.5)