##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632631.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20055
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.916828721017204	34.0	33.0	34.0	31.0	34.0
2	33.05699326851159	34.0	33.0	34.0	31.0	34.0
3	33.13044128646223	34.0	33.0	34.0	31.0	34.0
4	36.48272251308901	37.0	37.0	37.0	35.0	37.0
5	36.43345799052605	37.0	37.0	37.0	35.0	37.0
6	36.47823485415108	37.0	37.0	37.0	35.0	37.0
7	36.470356519571176	37.0	37.0	37.0	35.0	37.0
8	36.458090251807526	37.0	37.0	37.0	35.0	37.0
9	38.20822737471952	39.0	39.0	39.0	37.0	39.0
10	38.22129144851658	39.0	39.0	39.0	37.0	39.0
11	38.29374220892546	39.0	39.0	39.0	37.0	39.0
12	38.26631762652705	39.0	39.0	39.0	37.0	39.0
13	38.29768137621541	39.0	39.0	39.0	37.0	39.0
14	39.82268760907505	41.0	40.0	41.0	38.0	41.0
15	39.81819995013712	41.0	40.0	41.0	38.0	41.0
16	39.772226377461976	41.0	40.0	41.0	38.0	41.0
17	39.76369982547993	41.0	40.0	41.0	38.0	41.0
18	39.74684617302418	41.0	40.0	41.0	38.0	41.0
19	39.77023186237846	41.0	40.0	41.0	38.0	41.0
20	39.74724507604089	41.0	40.0	41.0	38.0	41.0
21	39.73133881824981	41.0	40.0	41.0	38.0	41.0
22	39.719172276240336	41.0	40.0	41.0	38.0	41.0
23	39.67718773373224	41.0	40.0	41.0	37.0	41.0
24	39.65061082024433	41.0	40.0	41.0	37.0	41.0
25	39.593667414609826	41.0	40.0	41.0	37.0	41.0
26	39.521316379955124	41.0	40.0	41.0	37.0	41.0
27	39.39596110695587	41.0	39.0	41.0	37.0	41.0
28	39.366891049613564	41.0	39.0	41.0	36.0	41.0
29	39.354874096235356	41.0	39.0	41.0	36.0	41.0
30	39.297432061829966	41.0	39.0	41.0	36.0	41.0
31	39.26392420842683	41.0	39.0	41.0	36.0	41.0
32	39.19715781600598	41.0	39.0	41.0	36.0	41.0
33	39.115233108950385	40.0	39.0	41.0	35.0	41.0
34	39.05454998753428	40.0	39.0	41.0	35.0	41.0
35	38.99222139117427	40.0	39.0	41.0	35.0	41.0
36	38.91862378459237	40.0	39.0	41.0	35.0	41.0
37	38.892695088506606	40.0	39.0	41.0	35.0	41.0
38	38.7870855148342	40.0	38.0	41.0	35.0	41.0
39	38.689553727250065	40.0	38.0	41.0	35.0	41.0
40	38.62557965594615	40.0	38.0	41.0	35.0	41.0
41	38.570032410870105	40.0	38.0	41.0	35.0	41.0
42	38.50296684118673	40.0	38.0	41.0	35.0	41.0
43	37.76704063824483	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	1.0
21	5.0
22	3.0
23	6.0
24	14.0
25	24.0
26	28.0
27	38.0
28	66.0
29	75.0
30	97.0
31	149.0
32	169.0
33	236.0
34	350.0
35	537.0
36	828.0
37	1668.0
38	4317.0
39	11443.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.20119670905011	16.459735726751433	12.814759411618052	27.524308152580403
2	21.44103714784343	19.272001994515083	32.291199202193965	26.99576165544752
3	21.18175018698579	20.024931438544005	29.21964597357268	29.573672400897532
4	15.951134380453752	15.17825978558963	33.33333333333333	35.53727250062329
5	16.978309648466716	34.08127648965345	32.45574669658439	16.484667165295438
6	35.81650461231613	32.73996509598604	14.719521316379955	16.724008975317876
7	29.967589129892797	27.74370481176764	19.31687858389429	22.971827474445274
8	27.67888307155323	30.969832959361753	20.30914983794565	21.042134131139367
9	28.047868362004486	13.054101221640488	16.99825479930192	41.8997756170531
10	19.291947145350285	23.754674644727	29.887808526551986	27.065569683370732
11	39.27200199451508	19.596110695587136	18.773373223635005	22.358514086262776
12	22.797307404637248	23.410620792819746	26.71154325604587	27.080528546497135
13	34.45026178010471	17.521815008726	20.75292944402892	27.274993767140366
14	24.223385689354277	19.650959860383942	22.5130890052356	33.61256544502618
15	28.93542757417103	24.512590376464722	19.970082273747195	26.581899775617053
16	26.332585390177016	23.335826477187734	21.8848167539267	28.44677137870855
17	27.524308152580403	23.221141859885318	21.31637995512341	27.93817003241087
18	25.529793069060087	21.610570929942657	23.914235851408627	28.94540014958863
19	28.606332585390177	22.642732485664425	23.07155322862129	25.67938170032411
20	28.7409623535278	21.047120418848166	23.585140862627775	26.626776364996264
21	27.13537771129394	22.278733482921965	21.74021441037148	28.845674395412612
22	27.903266018449262	22.62278733482922	21.19172276240339	28.282223884318125
23	28.41685365245575	22.398404387933184	22.174021441037148	27.010720518573923
24	28.27723759660932	21.650461231613065	21.864871603091498	28.207429568686116
25	29.23460483669908	21.57068062827225	22.62278733482922	26.571927200199454
26	28.656195462478184	21.894789329344302	21.99950137122912	27.44951383694839
27	27.354774370481177	21.19172276240339	24.367988032909498	27.08551483420593
28	25.799052605335326	22.907005734230864	22.722513089005236	28.57142857142857
29	27.718773373223637	23.465469957616556	21.81500872600349	27.000747943156323
30	27.030665669409125	22.393418100224384	23.49040139616056	27.08551483420593
31	27.30989778110197	22.413363251059586	22.802293692346048	27.4744452754924
32	25.993517825978557	22.44826726502119	22.4981301421092	29.06008476689105
33	27.155322862129143	21.725255547245077	23.68486661680379	27.43455497382199
34	26.8910496135627	21.411119421590627	23.86935926202942	27.828471702817254
35	26.482174021441036	23.19122413363251	23.729743206182995	26.596858638743452
36	26.153079032660187	22.348541510845177	25.18573921715283	26.31264023934181
37	27.37970580902518	21.346297681376218	23.854400398903017	27.419596110695586
38	26.003490401396164	21.17177761156819	25.420094739466464	27.404637247569184
39	25.340314136125652	20.468711044627273	26.387434554973822	27.803540264273245
40	24.67215158314635	21.416105709299426	27.48441785091	26.42732485664423
41	23.580154574918975	20.398903016704065	27.559212166542007	28.461730241834953
42	22.74245823984044	21.18673647469459	29.02518075292944	27.045624532535527
43	22.25380204437796	21.50585888805784	27.589129892794816	28.65120917476938
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	3.0
6	2.5
7	2.0
8	1.0
9	0.0
10	2.5
11	5.0
12	5.0
13	7.5
14	10.0
15	12.5
16	15.0
17	18.5
18	22.0
19	22.0
20	26.0
21	30.0
22	27.5
23	25.0
24	28.0
25	31.0
26	31.0
27	37.0
28	43.0
29	74.5
30	106.0
31	126.5
32	147.0
33	147.0
34	189.5
35	232.0
36	263.0
37	294.0
38	407.0
39	520.0
40	520.0
41	625.5
42	731.0
43	774.0
44	817.0
45	1087.0
46	1357.0
47	1357.0
48	1518.5
49	1680.0
50	1919.5
51	2159.0
52	2306.0
53	2453.0
54	2453.0
55	2233.0
56	2013.0
57	1907.0
58	1801.0
59	1651.5
60	1502.0
61	1502.0
62	1392.5
63	1283.0
64	1058.5
65	834.0
66	742.5
67	651.0
68	651.0
69	548.0
70	445.0
71	380.5
72	316.0
73	260.0
74	204.0
75	204.0
76	165.5
77	127.0
78	109.0
79	91.0
80	70.5
81	50.0
82	50.0
83	43.5
84	37.0
85	24.5
86	12.0
87	7.5
88	3.0
89	3.0
90	3.0
91	3.0
92	1.5
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	20055.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.75991024682125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.80098684210526	63.15632011967091
2	6.572094298245614	9.563699825479931
3	2.515076754385965	5.489902767389678
4	1.2129934210526316	3.5302916978309646
5	0.815515350877193	2.966841186736475
6	0.5071271929824561	2.213911742707554
7	0.3974780701754386	2.0244328097731237
8	0.3495065789473684	2.0344053851907256
9	0.1713267543859649	1.1219147344801794
>10	0.6578947368421052	7.898279730740463
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	48	0.23934181002243832	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	45	0.22438294689603588	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	37	0.18449264522562953	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	33	0.16454749439042632	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	30	0.14958863126402394	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	29	0.14460234355522314	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	28	0.13961605584642234	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	26	0.12964348042882073	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	26	0.12964348042882073	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	25	0.12465719272001993	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	25	0.12465719272001993	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	24	0.11967090501121916	TruSeq Adapter, Index 1 (95% over 22bp)
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	23	0.11468461730241836	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	23	0.11468461730241836	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	23	0.11468461730241836	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	22	0.10969832959361754	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	22	0.10969832959361754	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	22	0.10969832959361754	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	22	0.10969832959361754	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	21	0.10471204188481677	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	21	0.10471204188481677	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	21	0.10471204188481677	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	21	0.10471204188481677	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.004986287708800797	0.0
23	0.0	0.0	0.0	0.004986287708800797	0.0
24	0.0	0.0	0.0	0.004986287708800797	0.0
25	0.0	0.0	0.0	0.004986287708800797	0.0
26	0.0	0.0	0.0	0.004986287708800797	0.0
27	0.0	0.0	0.0	0.03989030167040638	0.0
28	0.0	0.0	0.0	0.10969832959361756	0.0
29	0.0	0.0	0.0	0.2493143854400399	0.0
30	0.0	0.0	0.0	0.418848167539267	0.0
31	0.0	0.0	0.0	0.8177511842433308	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	20	0.0017991454	37.0	37
GTCTCTT	100	0.0017258275	12.950001	37
TGTCTCT	165	3.3060132E-6	12.333334	37
CTGTCTC	185	1.18481E-5	11.0	36
>>END_MODULE