FastQCFastQC Report
Fri 10 Feb 2017
ERR1632630.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632630.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104122
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3120.29964848927219995No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2530.24298419162136725No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT1980.19016154126889612RNA PCR Primer, Index 29 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1930.18535948214594417No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1890.18151783484758266No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1800.1728741284262692No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1500.14406177368855766No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1470.1411805382147865No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1370.13157641996888264No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1330.12773477267052114No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1320.12677436084593074No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1250.12005147807379805No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1230.11813065442461727No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1200.11524941895084612No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1180.11332859530166536No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1170.11236818347707497No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1160.1114077716524846No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1130.10852653617871343No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1110.10660571252953266No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1070.10276406523117111No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1060.10180365340658074No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGTCG200.001834257937.022
GGTATCA1400.030.3928571
TTAGGAC250.00547369429.63
TAGGACC250.00547369429.64
TGGTCAT250.00547369429.614
GCCGTCT358.8151E-426.4285736
CGGCATA451.3119538E-424.66666636
AACTTAG451.3119538E-424.66666615
TATTGAT551.8812014E-523.5454544
ATATTGA400.001919476823.1250023
TGCCGTC400.001919476823.12500235
CGAACTT502.6792972E-422.213
ACTTAGT502.6792972E-422.216
GCCGAAC502.6792972E-422.211
GGCATAG502.6792972E-422.237
ACCCGAT502.6792972E-422.229
GTATCAA2000.021.2750022
CGTATGC450.003802830820.55555531
CTCGTAT450.003802830820.55555529
GAACTGT450.003802830820.55555536
Produced by FastQC (version 0.11.5)