##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632630.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	104122
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.93878335030061	34.0	33.0	34.0	31.0	34.0
2	33.085976066537334	34.0	33.0	34.0	31.0	34.0
3	33.16351011313651	34.0	33.0	34.0	31.0	34.0
4	36.490357465281114	37.0	37.0	37.0	35.0	37.0
5	36.44436334300148	37.0	37.0	37.0	35.0	37.0
6	36.48997330055128	37.0	37.0	37.0	35.0	37.0
7	36.49490981732967	37.0	37.0	37.0	35.0	37.0
8	36.4602677628167	37.0	37.0	37.0	35.0	37.0
9	38.23849906840053	39.0	39.0	39.0	37.0	39.0
10	38.25376961641152	39.0	39.0	39.0	37.0	39.0
11	38.329104319932384	39.0	39.0	39.0	37.0	39.0
12	38.28531914484931	39.0	39.0	39.0	37.0	39.0
13	38.3226215401164	39.0	39.0	39.0	37.0	39.0
14	39.837815255181425	41.0	40.0	41.0	38.0	41.0
15	39.83560630798486	41.0	40.0	41.0	38.0	41.0
16	39.796642400261234	41.0	40.0	41.0	38.0	41.0
17	39.80228001767158	41.0	40.0	41.0	38.0	41.0
18	39.80013829930274	41.0	40.0	41.0	38.0	41.0
19	39.80904131691669	41.0	40.0	41.0	38.0	41.0
20	39.77686752079292	41.0	40.0	41.0	38.0	41.0
21	39.74825685253837	41.0	40.0	41.0	38.0	41.0
22	39.71034939782179	41.0	40.0	41.0	38.0	41.0
23	39.666103225062905	41.0	40.0	41.0	37.0	41.0
24	39.645828931445806	41.0	40.0	41.0	37.0	41.0
25	39.60383012235646	41.0	40.0	41.0	37.0	41.0
26	39.5172393922514	41.0	40.0	41.0	37.0	41.0
27	39.41015347380957	41.0	39.0	41.0	37.0	41.0
28	39.37879602773669	41.0	39.0	41.0	37.0	41.0
29	39.3343385643764	41.0	39.0	41.0	36.0	41.0
30	39.3150054743474	41.0	39.0	41.0	36.0	41.0
31	39.248035957818715	41.0	39.0	41.0	36.0	41.0
32	39.19403200092199	41.0	39.0	41.0	36.0	41.0
33	39.12153051228367	40.0	39.0	41.0	35.0	41.0
34	39.09341925817791	40.0	39.0	41.0	35.0	41.0
35	38.98402835135706	40.0	39.0	41.0	35.0	41.0
36	38.93384683352222	40.0	39.0	41.0	35.0	41.0
37	38.87965079426058	40.0	39.0	41.0	35.0	41.0
38	38.80989608344058	40.0	38.0	41.0	35.0	41.0
39	38.70326155855631	40.0	38.0	41.0	35.0	41.0
40	38.62269261059142	40.0	38.0	41.0	35.0	41.0
41	38.56841973838382	40.0	38.0	41.0	35.0	41.0
42	38.50719348456618	40.0	38.0	41.0	35.0	41.0
43	37.76775321257755	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	1.0
20	5.0
21	16.0
22	29.0
23	40.0
24	87.0
25	114.0
26	136.0
27	203.0
28	255.0
29	353.0
30	488.0
31	638.0
32	889.0
33	1190.0
34	1757.0
35	2816.0
36	4468.0
37	8590.0
38	23297.0
39	58748.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.747719021916595	15.748833099633123	12.304796296652004	28.198651581798273
2	22.001114077716526	19.272584084055243	31.031866464339906	27.69443537388832
3	21.611186876932827	19.39167515030445	28.497339659245885	30.499798313516834
4	16.154126889610264	15.160100651159217	32.95653176081904	35.72924069841148
5	17.6686963369893	32.71066633372391	32.54835673536813	17.072280593918673
6	35.189489252991685	33.225447071704345	14.784579627744376	16.800484047559593
7	30.679395324715237	26.84831255642419	19.309079733389677	23.163212385470892
8	28.154472637867116	30.08970246441674	19.801770999404546	21.954053898311596
9	27.801041086417854	12.406599950058585	16.74189892625958	43.050460037263974
10	19.704769405120917	22.573519525172394	29.105280344211597	28.61643072549509
11	40.3910796949732	19.013272891415838	17.99331553370085	22.602331879910107
12	23.075814909433166	23.127677147961045	26.1885096329306	27.607998309675192
13	36.110524192773866	16.71884904246941	19.794087704807822	27.376539059948907
14	24.761337661589288	18.91819212078139	22.11732390849196	34.20314630913735
15	30.0339985785905	23.287105510843052	19.201513609035555	27.477382301530895
16	26.88480820575863	23.120954265188914	21.31345921130981	28.68077831774265
17	27.86730950231459	23.177618562839747	20.609477343885057	28.345594590960605
18	26.869441616565183	20.91872995140316	22.870286778970822	29.341541653060833
19	28.695184495111505	21.86473559862469	22.464992988993682	26.975086917270126
20	30.6332955571349	20.229154261347265	21.765813180691882	27.371737000825952
21	28.26203876222124	21.34323197787211	21.230863794395034	29.163865465511613
22	29.121607345229634	22.078907435508345	20.11390484239642	28.685580376865598
23	28.959297746873858	21.782140181709917	21.230863794395034	28.027698277021184
24	28.54055819135245	20.804440944276905	21.646722114442674	29.008278749927968
25	29.401087186185435	21.405658746470486	21.91179577802962	27.281458289314457
26	28.964099805996813	22.11732390849196	21.38068803903114	27.53788824648009
27	28.014252511476922	21.130020552813047	23.11903344153973	27.7366934941703
28	27.292983231209544	22.41505157411498	21.274082326501603	29.01788286817387
29	27.603196250552237	23.00954649353643	21.383569274504907	28.00368798140643
30	27.782793261750637	21.732198766831218	22.83955360058393	27.645454370834216
31	28.333109237240926	21.861854363150922	21.568928756650852	28.236107642957297
32	26.16642016096502	21.808071300973857	22.546627994083863	29.478880543977258
33	26.80701484796681	21.447916866752465	23.411959048039797	28.333109237240926
34	27.18637751868001	21.36147980253933	22.746393653598663	28.705749025181998
35	26.423810529955244	22.637867117419948	23.66646818155625	27.271854171068554
36	26.762835904035647	21.171318261270432	24.349320988840013	27.7165248458539
37	27.53788824648009	20.529763162444056	24.02374138030387	27.90860721077198
38	26.176024279210925	20.517277808724383	25.410576055012392	27.896121857052304
39	25.3894469948714	19.695165286875014	26.48431647490444	28.431071243349148
40	24.874666256890954	20.350166151245656	27.774149555329323	27.001018036534063
41	23.597318530185742	19.66251128483894	28.077639691899886	28.662530493075433
42	21.980945429400126	20.01210118898984	29.935076160657694	28.071877220952345
43	21.127139317339275	20.68727070167688	29.20420276214441	28.981387218839437
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	2.5
4	5.0
5	5.0
6	6.0
7	7.0
8	4.5
9	2.0
10	3.5
11	5.0
12	5.0
13	17.5
14	30.0
15	42.0
16	54.0
17	55.5
18	57.0
19	57.0
20	81.5
21	106.0
22	88.5
23	71.0
24	92.0
25	113.0
26	113.0
27	150.0
28	187.0
29	279.0
30	371.0
31	488.0
32	605.0
33	605.0
34	756.0
35	907.0
36	1071.0
37	1235.0
38	1620.0
39	2005.0
40	2005.0
41	2484.0
42	2963.0
43	3519.0
44	4075.0
45	5216.0
46	6357.0
47	6357.0
48	7263.5
49	8170.0
50	9287.0
51	10404.0
52	11134.5
53	11865.0
54	11865.0
55	11452.5
56	11040.0
57	10691.0
58	10342.0
59	9679.5
60	9017.0
61	9017.0
62	8396.0
63	7775.0
64	6439.0
65	5103.0
66	4484.0
67	3865.0
68	3865.0
69	3263.0
70	2661.0
71	2297.0
72	1933.0
73	1526.5
74	1120.0
75	1120.0
76	916.0
77	712.0
78	574.5
79	437.0
80	340.0
81	243.0
82	243.0
83	182.0
84	121.0
85	103.0
86	85.0
87	64.5
88	44.0
89	44.0
90	32.0
91	20.0
92	13.0
93	6.0
94	4.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	104122.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.24002612320163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.94253079775146	56.49430475788017
2	5.597416576964478	7.191563742532798
3	2.0302595383327353	3.912717773381226
4	1.1481880157875852	2.950385125141661
5	0.6936969262049995	2.2281554330496918
6	0.4515010166248057	1.7402662261577766
7	0.3827293385958617	1.7210579896659688
8	0.24219590958019377	1.244693724669138
9	0.19136466929793086	1.1063944219281228
>10	1.2109795479009688	14.784579627744376
>50	0.07475182394450425	3.3777683870843815
>100	0.03438583901447195	3.24811279076468
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	312	0.29964848927219995	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	253	0.24298419162136725	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	198	0.19016154126889612	RNA PCR Primer, Index 29 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	193	0.18535948214594417	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	189	0.18151783484758266	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	180	0.1728741284262692	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	150	0.14406177368855766	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	147	0.1411805382147865	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	137	0.13157641996888264	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	133	0.12773477267052114	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	132	0.12677436084593074	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	125	0.12005147807379805	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	123	0.11813065442461727	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	120	0.11524941895084612	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	118	0.11332859530166536	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	117	0.11236818347707497	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	116	0.1114077716524846	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	113	0.10852653617871343	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	111	0.10660571252953266	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	107	0.10276406523117111	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	106	0.10180365340658074	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	9.604118245903844E-4	0.0
18	0.0	0.0	0.0	9.604118245903844E-4	0.0
19	0.0	0.0	0.0	9.604118245903844E-4	0.0
20	0.0	0.0	0.0	9.604118245903844E-4	0.0
21	0.0	0.0	0.0	0.0019208236491807687	0.0
22	0.0	0.0	0.0	0.0038416472983615374	0.0
23	0.0	0.0	0.0	0.0038416472983615374	0.0
24	0.0	0.0	0.0	0.004802059122951922	0.0
25	0.0	0.0	0.0	0.006722882772132691	0.0
26	0.0	0.0	0.0	0.010564530070494228	0.0
27	0.0	0.0	0.0	0.034574825685253836	0.0
28	0.0	0.0	0.0	0.15078465646069034	0.0
29	0.0	0.0	0.0	0.3390253740804057	0.0
30	0.0	0.0	0.0	0.5407118572443864	0.0
31	0.0	0.0	0.0	1.0113136512936747	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTGTCG	20	0.0018342579	37.0	22
GGTATCA	140	0.0	30.392857	1
TTAGGAC	25	0.005473694	29.6	3
TAGGACC	25	0.005473694	29.6	4
TGGTCAT	25	0.005473694	29.6	14
GCCGTCT	35	8.8151E-4	26.42857	36
CGGCATA	45	1.3119538E-4	24.666666	36
AACTTAG	45	1.3119538E-4	24.666666	15
TATTGAT	55	1.8812014E-5	23.545454	4
ATATTGA	40	0.0019194768	23.125002	3
TGCCGTC	40	0.0019194768	23.125002	35
CGAACTT	50	2.6792972E-4	22.2	13
ACTTAGT	50	2.6792972E-4	22.2	16
GCCGAAC	50	2.6792972E-4	22.2	11
GGCATAG	50	2.6792972E-4	22.2	37
ACCCGAT	50	2.6792972E-4	22.2	29
GTATCAA	200	0.0	21.275002	2
CGTATGC	45	0.0038028308	20.555555	31
CTCGTAT	45	0.0038028308	20.555555	29
GAACTGT	45	0.0038028308	20.555555	36
>>END_MODULE