FastQCFastQC Report
Fri 10 Feb 2017
ERR1632629.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632629.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76823
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2360.30719966676646315No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1760.22909805657159965No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1490.19395233198391107No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1270.1653150749124611No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT1270.1653150749124611TruSeq Adapter, Index 8 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1210.15750491389297475No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1030.1340744308345157No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1030.1340744308345157No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1010.13147104382802025No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1000.13016935032477253No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA950.12366088280853389No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC950.12366088280853389No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA940.12235918930528616No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG910.118454108795543No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA870.1132473347825521No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC870.1132473347825521No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT860.11194564127930437No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT820.10673886726631347No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC800.10413548025981802No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA800.10413548025981802No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC790.10283378675657029No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC780.10153209325332256No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT780.10153209325332256No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC770.10023039975007485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCT355.5406235E-737.030
ATACGGC355.5406235E-737.029
TATACAC352.3596222E-531.71428537
TGGGACA250.00546485929.64
AGCCGCG1150.027.34782619
GGTAATA1150.027.34782625
CGCGGTA1150.027.34782622
AACTCCG1150.027.3478265
TAGCCAC358.7939407E-426.4285723
TGAATAG358.7939407E-426.4285719
ATGTTGG358.7939407E-426.428576
TAATACG1200.026.20833227
GCGGTAA1200.026.20833223
CCGCGGT1200.026.20833221
CGGTAAT1200.026.20833224
CGTGCCA1250.025.1610
GTGCCAG1250.025.1611
GGCTAAC1400.025.1071411
TGTGAAT451.3075728E-424.66666817
CTCCGTG1300.024.1923087
Produced by FastQC (version 0.11.5)