##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632629.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76823
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.955716387019514	34.0	33.0	34.0	31.0	34.0
2	33.10166226260365	34.0	33.0	34.0	31.0	34.0
3	33.17294299884149	34.0	33.0	34.0	31.0	34.0
4	36.488577639509	37.0	37.0	37.0	35.0	37.0
5	36.44684534579488	37.0	37.0	37.0	35.0	37.0
6	36.486455879098706	37.0	37.0	37.0	35.0	37.0
7	36.494760683649424	37.0	37.0	37.0	35.0	37.0
8	36.461150957395574	37.0	37.0	37.0	35.0	37.0
9	38.24464027700038	39.0	39.0	39.0	37.0	39.0
10	38.266964320581074	39.0	39.0	39.0	37.0	39.0
11	38.34377725420772	39.0	39.0	39.0	37.0	39.0
12	38.2970334405061	39.0	39.0	39.0	37.0	39.0
13	38.33268682556005	39.0	39.0	39.0	37.0	39.0
14	39.84142769743436	41.0	40.0	41.0	38.0	41.0
15	39.85012301003606	41.0	40.0	41.0	38.0	41.0
16	39.803886856800695	41.0	40.0	41.0	38.0	41.0
17	39.816278978951615	41.0	40.0	41.0	38.0	41.0
18	39.816057691056066	41.0	40.0	41.0	38.0	41.0
19	39.81249105085716	41.0	40.0	41.0	38.0	41.0
20	39.809301901774205	41.0	40.0	41.0	38.0	41.0
21	39.74532366608958	41.0	40.0	41.0	38.0	41.0
22	39.71551488486521	41.0	40.0	41.0	38.0	41.0
23	39.67516238626453	41.0	40.0	41.0	37.0	41.0
24	39.65473881519857	41.0	40.0	41.0	37.0	41.0
25	39.61147052315062	41.0	40.0	41.0	37.0	41.0
26	39.52853963005871	41.0	40.0	41.0	37.0	41.0
27	39.43031383830363	41.0	39.0	41.0	37.0	41.0
28	39.39425692826367	41.0	39.0	41.0	37.0	41.0
29	39.35032477252906	41.0	39.0	41.0	36.0	41.0
30	39.3122632544941	41.0	39.0	41.0	36.0	41.0
31	39.27202790830871	41.0	39.0	41.0	36.0	41.0
32	39.21574268122828	41.0	39.0	41.0	36.0	41.0
33	39.14754695859313	41.0	39.0	41.0	35.0	41.0
34	39.11120367598245	40.0	39.0	41.0	35.0	41.0
35	39.02975671348424	40.0	39.0	41.0	35.0	41.0
36	38.987998385900056	40.0	39.0	41.0	35.0	41.0
37	38.92450177681163	40.0	39.0	41.0	35.0	41.0
38	38.82848886401208	40.0	38.0	41.0	35.0	41.0
39	38.731135206904185	40.0	38.0	41.0	35.0	41.0
40	38.658904234408965	40.0	38.0	41.0	35.0	41.0
41	38.60359527745597	40.0	38.0	41.0	35.0	41.0
42	38.54790882938703	40.0	38.0	41.0	35.0	41.0
43	37.809549223539825	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	3.0
21	14.0
22	14.0
23	32.0
24	52.0
25	66.0
26	108.0
27	170.0
28	187.0
29	279.0
30	357.0
31	464.0
32	646.0
33	837.0
34	1271.0
35	2027.0
36	3249.0
37	6301.0
38	16928.0
39	43816.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.33467841662002	16.45080249404475	12.367389974356637	27.847129114978586
2	21.66408497455189	19.20388425341369	31.5868945498093	27.545136222225114
3	21.692722231623343	19.228616429975396	28.522708043164158	30.555953295237103
4	16.109758796193848	15.400335836923837	33.385834971297655	35.10407039558466
5	17.674394387097614	32.29761920258256	32.79746950782968	17.23051690249014
6	35.08324329953269	33.27128594301186	14.80936698644937	16.836103771006076
7	30.876169897036043	26.851333584994073	19.23382320398839	23.03867331398149
8	27.685719120575868	30.449214427970787	19.90289366465772	21.962172786795623
9	28.07622717155019	12.625125287999689	17.155018679301772	42.14362886114835
10	19.770120927326452	22.747093969254	29.259466566002367	28.22331853741718
11	39.751116202179034	19.383517956861876	18.184658240370723	22.680707600588367
12	23.02175129843927	23.019147911432775	26.4373950509613	27.521705739166656
13	35.85384585345535	17.309920206188252	20.01874438644677	26.817489553909635
14	24.534319149213125	19.509782226676904	22.490660349114197	33.46523827499577
15	29.846530335967092	23.620530309933223	19.293701105137785	27.2392382489619
16	26.39443916535413	23.611418455410487	21.882769483097512	28.111372896137876
17	27.375916066802912	23.088137667104903	21.295705713132786	28.2402405529594
18	26.761516733269985	20.928628145216926	23.028259765955507	29.28159535555758
19	29.045988831469742	21.64455957200318	22.544029782747355	26.765421813779728
20	30.313838303633027	20.519896385197143	22.079325202087915	27.086940109081915
21	28.25195579448863	21.563854574801816	21.531312237220625	28.65287739348893
22	28.60731812087526	22.502375590643428	20.596696301888755	28.293609986592553
23	28.893690691589757	21.6888171511136	21.82028819494162	27.59720396235502
24	28.409460708381605	20.82319097145386	22.287596162607553	28.47975215755698
25	29.200890358356226	21.77472892232795	21.989508350363824	27.034872368952005
26	28.73748747120003	22.47113494656548	21.385522564856878	27.405855017377608
27	28.05930515600797	21.53261393072387	23.0907410541114	27.317339859156764
28	27.162438332270284	22.545331476250603	21.523502076201137	28.768728115277973
29	27.70133944261484	23.202686695390703	21.496166512632936	27.599807349361523
30	27.10516381812738	21.955664319279382	23.584082891842286	27.355088970750945
31	28.305325228121784	21.816383114431876	22.1170743136821	27.761217343764237
32	25.824297410931624	21.98560326985408	22.71194824466631	29.478151074547988
33	26.644364317977686	21.65236973302266	23.664787889043644	28.038478059956002
34	27.020553740416283	21.67580021608112	23.188368066854977	28.11527797664762
35	26.261666428022856	22.665087278549393	23.805370787394402	27.267875506033352
36	26.8682556005363	21.250146440519117	24.482251409083215	27.39934654986137
37	27.62193613891673	20.78934694036942	23.992814651862073	27.595902268851773
38	26.14972078674355	20.462621871054242	25.485857100087213	27.901800242114994
39	25.467633391041744	19.453809406037255	26.791455683844685	28.28710151907632
40	25.1200812256746	20.16193067180402	27.921325644663707	26.79666245785767
41	23.370605157309658	19.58397875636203	28.47064030303425	28.574775783294065
42	22.019447300938523	20.09163922262864	30.2123062103797	27.676607266053132
43	21.342566679249703	20.76070968329797	29.147520924723064	28.74920271272926
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	3.0
4	6.0
5	6.0
6	4.0
7	2.0
8	4.0
9	6.0
10	6.5
11	7.0
12	7.0
13	16.5
14	26.0
15	39.0
16	52.0
17	54.0
18	56.0
19	56.0
20	71.5
21	87.0
22	91.5
23	96.0
24	105.0
25	114.0
26	114.0
27	134.0
28	154.0
29	235.5
30	317.0
31	401.5
32	486.0
33	486.0
34	586.0
35	686.0
36	829.0
37	972.0
38	1231.5
39	1491.0
40	1491.0
41	1838.0
42	2185.0
43	2599.0
44	3013.0
45	3948.0
46	4883.0
47	4883.0
48	5600.0
49	6317.0
50	7127.0
51	7937.0
52	8436.5
53	8936.0
54	8936.0
55	8590.5
56	8245.0
57	7865.0
58	7485.0
59	7028.5
60	6572.0
61	6572.0
62	6022.5
63	5473.0
64	4560.0
65	3647.0
66	3188.0
67	2729.0
68	2729.0
69	2278.0
70	1827.0
71	1523.5
72	1220.0
73	1008.0
74	796.0
75	796.0
76	623.0
77	450.0
78	355.5
79	261.0
80	194.5
81	128.0
82	128.0
83	106.5
84	85.0
85	63.0
86	41.0
87	30.5
88	20.0
89	20.0
90	14.5
91	9.0
92	5.0
93	1.0
94	2.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	76823.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.7449331580386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.38012424857757	56.57420303815264
2	6.0093689056876896	7.781523762414902
3	2.0687991314662537	4.018327844525728
4	1.2042863748768573	3.1188576337815497
5	0.72578861657854	2.349556773362144
6	0.48050825308108325	1.866628483657238
7	0.3578680713323549	1.6219101050466658
8	0.257343332194053	1.3329341473256706
9	0.1769235408834114	1.0309412545721983
>10	1.2264018174872837	14.435780951017275
>50	0.09449325479000381	4.251330981607071
>100	0.01809445304489435	1.6180050245369226
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	236	0.30719966676646315	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	176	0.22909805657159965	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	149	0.19395233198391107	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	127	0.1653150749124611	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	127	0.1653150749124611	TruSeq Adapter, Index 8 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	121	0.15750491389297475	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	103	0.1340744308345157	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	103	0.1340744308345157	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	101	0.13147104382802025	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	100	0.13016935032477253	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	95	0.12366088280853389	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	95	0.12366088280853389	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	94	0.12235918930528616	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	91	0.118454108795543	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	87	0.1132473347825521	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	87	0.1132473347825521	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	86	0.11194564127930437	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	82	0.10673886726631347	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	80	0.10413548025981802	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	80	0.10413548025981802	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	79	0.10283378675657029	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	78	0.10153209325332256	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	78	0.10153209325332256	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	77	0.10023039975007485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0013016935032477252	0.0	0.0	0.0	0.0
16	0.0013016935032477252	0.0	0.0	0.0013016935032477252	0.0
17	0.0013016935032477252	0.0	0.0	0.0013016935032477252	0.0
18	0.0013016935032477252	0.0	0.0	0.0013016935032477252	0.0
19	0.0013016935032477252	0.0	0.0	0.0013016935032477252	0.0
20	0.0013016935032477252	0.0	0.0	0.0013016935032477252	0.0
21	0.0013016935032477252	0.0	0.0	0.0013016935032477252	0.0
22	0.0013016935032477252	0.0	0.0	0.0026033870064954504	0.0
23	0.0013016935032477252	0.0	0.0	0.003905080509743176	0.0
24	0.0013016935032477252	0.0	0.0	0.003905080509743176	0.0
25	0.0013016935032477252	0.0	0.0	0.005206774012990901	0.0013016935032477252
26	0.0013016935032477252	0.0	0.0	0.009111854522734077	0.0013016935032477252
27	0.0013016935032477252	0.0	0.0	0.03254233758119313	0.0013016935032477252
28	0.0013016935032477252	0.0	0.0	0.15099644637673612	0.0013016935032477252
29	0.0013016935032477252	0.0	0.0	0.31500982778594955	0.0013016935032477252
30	0.0013016935032477252	0.0	0.0	0.5375994168413105	0.0013016935032477252
31	0.0013016935032477252	0.0	0.0	1.1389818153417597	0.0013016935032477252
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGCT	35	5.5406235E-7	37.0	30
ATACGGC	35	5.5406235E-7	37.0	29
TATACAC	35	2.3596222E-5	31.714285	37
TGGGACA	25	0.005464859	29.6	4
AGCCGCG	115	0.0	27.347826	19
GGTAATA	115	0.0	27.347826	25
CGCGGTA	115	0.0	27.347826	22
AACTCCG	115	0.0	27.347826	5
TAGCCAC	35	8.7939407E-4	26.42857	23
TGAATAG	35	8.7939407E-4	26.42857	19
ATGTTGG	35	8.7939407E-4	26.42857	6
TAATACG	120	0.0	26.208332	27
GCGGTAA	120	0.0	26.208332	23
CCGCGGT	120	0.0	26.208332	21
CGGTAAT	120	0.0	26.208332	24
CGTGCCA	125	0.0	25.16	10
GTGCCAG	125	0.0	25.16	11
GGCTAAC	140	0.0	25.107141	1
TGTGAAT	45	1.3075728E-4	24.666668	17
CTCCGTG	130	0.0	24.192308	7
>>END_MODULE