FastQCFastQC Report
Fri 10 Feb 2017
ERR1632628.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632628.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17673
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA570.3225258869461891No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA440.2489673513268828No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT410.23199230464550444TruSeq Adapter, Index 6 (95% over 21bp)
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC300.16975046681378375No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT280.15843376902619816No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA280.15843376902619816No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT280.15843376902619816No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG250.1414587223448198No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT230.1301420245572342No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC230.1301420245572342No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT230.1301420245572342No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA230.1301420245572342No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC220.1244836756634414No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA220.1244836756634414No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA220.1244836756634414No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA210.1188253267696486No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA200.11316697787585582No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT190.10750862898206304No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC180.10185028008827025No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA180.10185028008827025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA303.459136E-430.8333342
GGTATCA303.459136E-430.8333341
CCAGCAG250.005352779329.614
GCGGTAA250.005352779329.623
AACTCCG250.005352779329.65
TAACTCC250.005352779329.64
CTAACTC250.005352779329.63
GCTAACT250.005352779329.62
CCCTGTC250.005352779329.635
CGCGGTA250.005352779329.622
CGTGCCA250.005352779329.610
TCCGTGC250.005352779329.68
GTAATAC250.005352779329.626
CGGTAAT250.005352779329.624
GGTAATA250.005352779329.625
CCGCGGT250.005352779329.621
GTGCCAG250.005352779329.611
GCCAGCA358.5267803E-426.42857213
TGCCAGC450.00368076520.55555512
TTTAGTG450.00368076520.5555553
Produced by FastQC (version 0.11.5)