FastQCFastQC Report
Fri 10 Feb 2017
ERR1632627.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632627.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8228
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT620.7535245503159942Illumina PCR Primer Index 10 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA330.4010695187165776No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC260.3159941662615459Illumina PCR Primer Index 10 (95% over 22bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA200.24307243558580457No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT190.23091881380651433No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT190.23091881380651433No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC170.2066115702479339No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA160.19445794846864364No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC160.19445794846864364No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA160.19445794846864364No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC150.18230432668935342No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC150.18230432668935342No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA150.18230432668935342No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA130.15799708313077296No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT130.15799708313077296No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC120.14584346135148274No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA120.14584346135148274No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC120.14584346135148274No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC120.14584346135148274No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT110.1336898395721925No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC100.12153621779290229No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG100.12153621779290229No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA100.12153621779290229No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC100.12153621779290229No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC90.10938259601361207No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA90.10938259601361207No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT90.10938259601361207No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC90.10938259601361207No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA90.10938259601361207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTCT901.673181E-618.537
CTGTCTC1008.986303E-514.79999936
Produced by FastQC (version 0.11.5)