##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632627.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8228
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.972046669907634	34.0	33.0	34.0	31.0	34.0
2	33.11144871171609	34.0	33.0	34.0	31.0	34.0
3	33.20491006319883	34.0	34.0	34.0	31.0	34.0
4	36.48225571220224	37.0	37.0	37.0	35.0	37.0
5	36.48772484200292	37.0	37.0	37.0	35.0	37.0
6	36.519810403500244	37.0	37.0	37.0	35.0	37.0
7	36.49586776859504	37.0	37.0	37.0	35.0	37.0
8	36.473505104521145	37.0	37.0	37.0	35.0	37.0
9	38.30736509479825	39.0	39.0	39.0	37.0	39.0
10	38.303840544482256	39.0	39.0	39.0	37.0	39.0
11	38.3509965969859	39.0	39.0	39.0	37.0	39.0
12	38.31380651434127	39.0	39.0	39.0	37.0	39.0
13	38.36363636363637	39.0	39.0	39.0	37.0	39.0
14	39.87019931939718	41.0	40.0	41.0	38.0	41.0
15	39.84175984443364	41.0	40.0	41.0	38.0	41.0
16	39.84467671366067	41.0	40.0	41.0	38.0	41.0
17	39.81720952843948	41.0	40.0	41.0	38.0	41.0
18	39.846378220709774	41.0	40.0	41.0	38.0	41.0
19	39.858653378706855	41.0	40.0	41.0	38.0	41.0
20	39.803962080700046	41.0	40.0	41.0	38.0	41.0
21	39.77977637335926	41.0	40.0	41.0	38.0	41.0
22	39.723505104521145	41.0	40.0	41.0	38.0	41.0
23	39.659333981526494	41.0	40.0	41.0	37.0	41.0
24	39.685342732134174	41.0	40.0	41.0	37.0	41.0
25	39.655201750121535	41.0	40.0	41.0	37.0	41.0
26	39.52175498298493	41.0	40.0	41.0	37.0	41.0
27	39.42744287797764	41.0	39.0	41.0	37.0	41.0
28	39.388794360719494	41.0	39.0	41.0	37.0	41.0
29	39.428050559066605	41.0	39.0	41.0	37.0	41.0
30	39.37299465240642	41.0	39.0	41.0	36.0	41.0
31	39.29253767622752	41.0	39.0	41.0	36.0	41.0
32	39.17476908118619	41.0	39.0	41.0	35.0	41.0
33	39.138672824501704	41.0	39.0	41.0	35.0	41.0
34	39.10172581429266	41.0	39.0	41.0	35.0	41.0
35	38.99671852211959	40.0	39.0	41.0	35.0	41.0
36	38.96268838113758	40.0	39.0	41.0	35.0	41.0
37	38.88514827418571	40.0	39.0	41.0	35.0	41.0
38	38.80748663101604	40.0	38.0	41.0	35.0	41.0
39	38.69907632474477	40.0	38.0	41.0	35.0	41.0
40	38.60658726300438	40.0	38.0	41.0	35.0	41.0
41	38.563441905687895	40.0	38.0	41.0	35.0	41.0
42	38.49586776859504	40.0	38.0	41.0	35.0	41.0
43	37.72739426349052	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	2.0
23	6.0
24	7.0
25	10.0
26	8.0
27	9.0
28	26.0
29	24.0
30	44.0
31	53.0
32	72.0
33	93.0
34	146.0
35	216.0
36	335.0
37	721.0
38	1674.0
39	4781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2790471560525	15.106951871657753	12.603305785123966	29.010695187165776
2	22.070977151191055	18.522119591638308	30.17744287797764	29.229460379193
3	21.463296062226544	18.874574623237724	28.087019931939714	31.57510938259601
4	15.982012639766651	14.9246475449684	32.7175498298493	36.37578998541565
5	18.279047156052503	33.19154107924162	30.991735537190085	17.5376762275158
6	35.53719008264463	32.960622265435106	14.803111327175497	16.699076324744773
7	30.89450656295576	27.10257656781721	18.133203694701024	23.86971317452601
8	28.913466212931453	29.812834224598934	19.299951385512884	21.973748176956732
9	27.236266407389405	12.092853670393778	16.626154594069032	44.04472532814779
10	20.588235294117645	22.39912493923189	27.843947496353916	29.168692270296546
11	41.32231404958678	18.339815264948957	17.379679144385026	22.95819154107924
12	23.517258142926593	23.82109868740885	24.744773942634904	27.916869227029657
13	37.8099173553719	16.97860962566845	18.47350510452115	26.737967914438503
14	25.887214389888186	18.49781234807973	21.937287311618864	33.67768595041322
15	30.760816723383567	23.39572192513369	17.780748663101605	28.062712688381136
16	26.507049100631992	23.699562469615945	20.26008750607681	29.533300923675256
17	27.67379679144385	23.699562469615945	20.333009236752552	28.29363150218765
18	28.415167719980555	19.6524064171123	20.989304812834224	30.943121050072918
19	28.864851725814294	21.669907632474477	21.92513368983957	27.540106951871657
20	31.50218765192027	20.527467185221195	20.478852698104035	27.491492464754497
21	29.484686436558093	19.555177442877977	20.333009236752552	30.62712688381137
22	29.508993680116674	22.241127856101116	19.336412250850753	28.913466212931453
23	30.70004861448712	20.442391832766162	20.442391832766162	28.415167719980555
24	28.92561983471074	19.956246961594555	21.35391346621293	29.764219737481767
25	29.423918327661642	21.12299465240642	22.27758872143899	27.17549829849295
26	29.3509965969859	21.92513368983957	20.563928050559067	28.159941662615463
27	27.187651920272245	21.596985901798735	23.201263976665047	28.014098201263977
28	27.588721438988816	22.046669907632474	20.867768595041323	29.496840058337387
29	27.333495381623724	22.92173067574137	20.636849781234808	29.107924161400096
30	28.22070977151191	21.12299465240642	23.675255226057367	26.981040350024305
31	29.229460379193	21.17160913952358	21.050072921730674	28.548857559552747
32	25.94798249878464	21.633446767136608	22.581429265921244	29.837141468157512
33	26.288283908604765	21.23237724842003	23.894020418084587	28.585318424890616
34	26.725814292659212	20.66115702479339	23.43218279047156	29.18084589207584
35	25.559066601847352	22.107438016528928	24.562469615945552	27.77102576567817
36	28.135634419056878	20.612542537676227	23.8818667963053	27.369956246961596
37	27.02965483714147	19.73748176956733	24.46524064171123	28.767622751579967
38	26.507049100631992	19.968400583373846	25.35245503159942	28.17209528439475
39	25.315994166261547	18.351968886728244	26.64073894020418	29.691298006806026
40	24.659698590179875	19.372873116188625	28.208556149732622	27.758872143898884
41	24.53816237238697	18.242586290714634	28.986387943607195	28.2328633932912
42	22.654350996596985	18.7773456490034	30.639280505590666	27.929022848808945
43	21.037919299951387	19.12980068060282	30.62712688381137	29.205153135634422
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.0
15	3.0
16	4.0
17	4.5
18	5.0
19	5.0
20	6.0
21	7.0
22	6.5
23	6.0
24	6.0
25	6.0
26	6.0
27	10.5
28	15.0
29	19.5
30	24.0
31	27.0
32	30.0
33	30.0
34	45.0
35	60.0
36	66.0
37	72.0
38	88.5
39	105.0
40	105.0
41	144.5
42	184.0
43	215.5
44	247.0
45	368.5
46	490.0
47	490.0
48	558.5
49	627.0
50	722.0
51	817.0
52	860.5
53	904.0
54	904.0
55	945.5
56	987.0
57	917.5
58	848.0
59	814.0
60	780.0
61	780.0
62	692.5
63	605.0
64	520.5
65	436.0
66	380.5
67	325.0
68	325.0
69	276.5
70	228.0
71	200.5
72	173.0
73	138.0
74	103.0
75	103.0
76	76.5
77	50.0
78	40.5
79	31.0
80	28.5
81	26.0
82	26.0
83	21.0
84	16.0
85	11.5
86	7.0
87	6.5
88	6.0
89	6.0
90	3.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8228.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.0734078755469
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.33718347401155	74.96353913466213
2	4.871908781282393	7.997083130772971
3	1.6289056715533836	4.010695187165775
4	0.7700281356434178	2.5279533300923673
5	0.37020583444395083	1.5192027224112785
6	0.2517399674218866	1.2396694214876034
7	0.0888494002665482	0.5104521147301896
8	0.2517399674218866	1.6528925619834711
9	0.07404116688879016	0.5469129800680603
>10	0.3405893676884348	4.27807486631016
>50	0.014808233377758034	0.7535245503159942
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	62	0.7535245503159942	Illumina PCR Primer Index 10 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	33	0.4010695187165776	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC	26	0.3159941662615459	Illumina PCR Primer Index 10 (95% over 22bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	20	0.24307243558580457	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	19	0.23091881380651433	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	19	0.23091881380651433	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	17	0.2066115702479339	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	16	0.19445794846864364	No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	16	0.19445794846864364	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	16	0.19445794846864364	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	15	0.18230432668935342	No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	15	0.18230432668935342	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	15	0.18230432668935342	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	13	0.15799708313077296	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	13	0.15799708313077296	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	12	0.14584346135148274	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	12	0.14584346135148274	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	12	0.14584346135148274	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	12	0.14584346135148274	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	11	0.1336898395721925	No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC	10	0.12153621779290229	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	10	0.12153621779290229	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	10	0.12153621779290229	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	10	0.12153621779290229	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	9	0.10938259601361207	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	9	0.10938259601361207	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	9	0.10938259601361207	No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	9	0.10938259601361207	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	9	0.10938259601361207	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.012153621779290228	0.0
27	0.0	0.0	0.0	0.060768108896451144	0.0
28	0.0	0.0	0.0	0.24307243558580457	0.0
29	0.0	0.0	0.0	0.5226057365094798	0.0
30	0.0	0.0	0.0	0.7656781720952844	0.0
31	0.0	0.0	0.0	1.3612056392805056	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCTCT	90	1.673181E-6	18.5	37
CTGTCTC	100	8.986303E-5	14.799999	36
>>END_MODULE