FastQCFastQC Report
Fri 10 Feb 2017
ERR1632626.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632626.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76731
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1490.19418487964447223No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1480.1928816254186704No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT1260.16421003245103022TruSeq Adapter, Index 3 (95% over 21bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1160.15117749019301197No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1110.1446612190640028No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1060.13814494793499368No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1040.13553843948339003No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1000.1303254225801827No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT990.12902216835438088No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA930.12120264299956994No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA900.11729288032216445No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA860.11207986341895713No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC840.10947335496735348No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT830.10817010074155166No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG800.10426033806414617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAAAC200.001831248437.025
AACATCT200.001831248437.029
TATACAC200.001831248437.037
TGAACCG303.5685228E-430.8333325
AGTGAGC303.5685228E-430.8333328
GGTATCA1050.029.952381
TACCCCG250.00546481529.65
CATCTAA250.00546481529.631
TGAAACA250.00546481529.626
ACAGGAA250.00546481529.628
TCTAAGT250.00546481529.633
GAAACAT250.00546481529.627
GAACCGT250.00546481529.66
ACATCTA250.00546481529.630
CACAATA250.00546481529.634
GGCGAAC250.00546481529.610
GGTGGAT358.793833E-426.42857210
TACCACG652.6290654E-622.769234
GGATACC652.6290654E-622.769231
AACTCCG652.6290654E-622.769235
Produced by FastQC (version 0.11.5)