##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632626.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76731
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02533526214959	34.0	33.0	34.0	31.0	34.0
2	33.166490727346186	34.0	33.0	34.0	31.0	34.0
3	33.25128044727685	34.0	34.0	34.0	31.0	34.0
4	36.53085454379586	37.0	37.0	37.0	35.0	37.0
5	36.500944859313705	37.0	37.0	37.0	35.0	37.0
6	36.54237531115194	37.0	37.0	37.0	35.0	37.0
7	36.53523347799455	37.0	37.0	37.0	35.0	37.0
8	36.51680546324172	37.0	37.0	37.0	35.0	37.0
9	38.302615631231184	39.0	39.0	39.0	37.0	39.0
10	38.3340892207843	39.0	39.0	39.0	37.0	39.0
11	38.41878771291916	39.0	39.0	39.0	37.0	39.0
12	38.37077582724062	39.0	39.0	39.0	37.0	39.0
13	38.405807300830176	39.0	39.0	39.0	37.0	39.0
14	39.96680611486883	41.0	40.0	41.0	38.0	41.0
15	39.950906413314044	41.0	40.0	41.0	38.0	41.0
16	39.913555147202565	41.0	40.0	41.0	38.0	41.0
17	39.92089246849383	41.0	40.0	41.0	38.0	41.0
18	39.91230402314579	41.0	40.0	41.0	38.0	41.0
19	39.90027498664164	41.0	40.0	41.0	38.0	41.0
20	39.87645149939399	41.0	40.0	41.0	38.0	41.0
21	39.86597333541854	41.0	40.0	41.0	38.0	41.0
22	39.83693683126768	41.0	40.0	41.0	38.0	41.0
23	39.78689186899688	41.0	40.0	41.0	38.0	41.0
24	39.77031447524469	41.0	40.0	41.0	38.0	41.0
25	39.7284018193429	41.0	40.0	41.0	38.0	41.0
26	39.652734878992845	41.0	40.0	41.0	37.0	41.0
27	39.56668100246315	41.0	40.0	41.0	37.0	41.0
28	39.53296581564166	41.0	40.0	41.0	37.0	41.0
29	39.50091879422919	41.0	40.0	41.0	37.0	41.0
30	39.444396658456164	41.0	39.0	41.0	37.0	41.0
31	39.39405194771344	41.0	39.0	41.0	36.0	41.0
32	39.3402666458146	41.0	39.0	41.0	36.0	41.0
33	39.268222752212274	41.0	39.0	41.0	36.0	41.0
34	39.224094564126624	41.0	39.0	41.0	35.0	41.0
35	39.17080449883359	41.0	39.0	41.0	35.0	41.0
36	39.12323571959182	41.0	39.0	41.0	35.0	41.0
37	39.05989756421785	41.0	39.0	41.0	35.0	41.0
38	38.990603537031966	40.0	39.0	41.0	35.0	41.0
39	38.89684742802778	40.0	39.0	41.0	35.0	41.0
40	38.827045131693836	40.0	38.0	41.0	35.0	41.0
41	38.76516662105277	40.0	38.0	41.0	35.0	41.0
42	38.711915653386505	40.0	38.0	41.0	35.0	41.0
43	37.963626174557874	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	0.0
19	2.0
20	6.0
21	3.0
22	11.0
23	25.0
24	39.0
25	65.0
26	90.0
27	139.0
28	198.0
29	240.0
30	338.0
31	440.0
32	565.0
33	788.0
34	1176.0
35	1804.0
36	3013.0
37	5787.0
38	14960.0
39	47040.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.17550924658873	16.194237009813502	12.46823317824608	28.162020565351682
2	21.60404530111689	18.782499902255935	31.517900196791388	28.095554599835793
3	21.645749436342548	19.110919967157994	27.950893380771785	31.29243721572767
4	16.305013619006658	15.129478307333411	32.464062764723515	36.10144530893641
5	18.15302811119365	31.738150160951896	32.767720999335346	17.341100728519113
6	36.304752968161495	32.030079107531506	14.890982784011678	16.774185140295316
7	31.524416467920396	26.61636105355072	19.202147762964124	22.657074715564764
8	28.603823747898506	30.328029088634317	19.332473185544305	21.735673977922872
9	28.1033741251906	12.758858870599887	16.999648121359034	42.13811888285048
10	19.982797044219417	22.80955545998358	29.531740756669407	27.675906739127605
11	40.26273605192165	18.627412649385516	18.21558431403214	22.894266984660696
12	23.25917816788521	23.070206305143945	26.088543092100974	27.58207243486987
13	35.76390246445374	17.093482425616767	19.89287250263909	27.249742607290408
14	24.78659212052495	19.0431507474163	22.08233960198616	34.087917530072595
15	29.94487234624858	23.448150030626476	19.331169931318502	27.275807691806442
16	26.84573379729184	23.06759979669234	21.601438792665284	28.48522761335054
17	27.58858870599888	23.100181152337388	21.364246523569353	27.946983618094386
18	27.51039345245077	21.011064628377056	22.352113226727138	29.12642869244503
19	28.7419686958335	21.971562992793004	22.323441633759497	26.963026677614003
20	29.585174179927275	20.915927069893524	22.199632482308324	27.299266267870877
21	28.18938890409352	21.83341804485801	21.394221370762796	28.582971680285674
22	28.874900626865283	22.34429370137233	20.926353103699938	27.854452568062456
23	28.847532288123446	21.50369472573015	21.55452164053642	28.09425134560999
24	28.659863679607984	21.044949238247902	21.78519763850334	28.50998944364077
25	28.971341439574616	21.456777573601283	21.750009774406692	27.821871212417403
26	28.521718731672983	22.34429370137233	21.87642543430947	27.257562132645212
27	27.71109460322425	21.549308623633213	22.882537696628482	27.857059076514055
28	26.677614002163402	22.376875057017372	21.86208963782565	29.08342130299357
29	27.877911144126884	22.826497764919	21.846450587116028	27.44914050383808
30	27.77365080606274	21.286051270021243	22.88123444240268	28.05906348151334
31	28.289739479480264	21.756526045535704	22.07582333085715	27.877911144126884
32	26.76232552684052	21.467203607407697	22.2530659055662	29.517404960185583
33	26.800119899388775	21.30429682918247	23.674916265915993	28.220667005512766
34	27.193702675580926	21.018884153731868	23.091058372756773	28.696354797930436
35	26.488642139422137	22.642738919080944	23.781783112431743	27.086835829065176
36	26.964329931839803	21.0397362213447	24.112809685785404	27.883124161030093
37	27.129843218516637	20.680038055023395	24.334362904171716	27.855755822288252
38	25.972553466004612	20.295578058411852	25.59982275742529	28.13204571815824
39	25.38608906439379	19.468011625027696	26.642426138066753	28.50347317251176
40	25.147593541072055	20.445452294379066	27.395707080580205	27.01124708396867
41	23.913411789237728	19.632221657478723	28.120316430126024	28.33405012315752
42	22.51762651340397	20.617481852184905	28.895752694478112	27.969138939933014
43	21.86860590895466	20.883345714248478	28.61555303593072	28.632495340866143
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	2.0
4	2.0
5	2.0
6	2.0
7	2.0
8	1.5
9	1.0
10	5.0
11	9.0
12	9.0
13	11.5
14	14.0
15	32.0
16	50.0
17	55.5
18	61.0
19	61.0
20	66.0
21	71.0
22	68.0
23	65.0
24	72.0
25	79.0
26	79.0
27	106.0
28	133.0
29	184.0
30	235.0
31	308.5
32	382.0
33	382.0
34	538.5
35	695.0
36	829.5
37	964.0
38	1263.0
39	1562.0
40	1562.0
41	1913.0
42	2264.0
43	2666.0
44	3068.0
45	4023.0
46	4978.0
47	4978.0
48	5613.0
49	6248.0
50	7111.5
51	7975.0
52	8090.5
53	8206.0
54	8206.0
55	7977.0
56	7748.0
57	7470.0
58	7192.0
59	6837.0
60	6482.0
61	6482.0
62	6064.0
63	5646.0
64	4853.5
65	4061.0
66	3532.0
67	3003.0
68	3003.0
69	2549.0
70	2095.0
71	1749.0
72	1403.0
73	1107.5
74	812.0
75	812.0
76	667.0
77	522.0
78	422.0
79	322.0
80	255.5
81	189.0
82	189.0
83	149.5
84	110.0
85	84.0
86	58.0
87	35.5
88	13.0
89	13.0
90	9.0
91	5.0
92	3.5
93	2.0
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	76731.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.62474097822262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.69265218699661	66.77223025895661
2	4.951055883029755	7.290404139135422
3	1.5276228913316694	3.3741251906009304
4	0.755845857008833	2.2259582176695205
5	0.44961322641743223	1.6551328667683205
6	0.32039367709273714	1.4153340892207842
7	0.21595595914538085	1.1129791088347603
8	0.15223124988936682	0.8966389073516571
9	0.10266758713468925	0.6802987058685538
>10	0.7629263802595012	10.630644719865504
>50	0.05664418600534579	2.825455161538361
>100	0.012390915688669395	1.1207986341895713
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	149	0.19418487964447223	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	148	0.1928816254186704	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	126	0.16421003245103022	TruSeq Adapter, Index 3 (95% over 21bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	116	0.15117749019301197	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	111	0.1446612190640028	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	106	0.13814494793499368	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	104	0.13553843948339003	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	100	0.1303254225801827	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	99	0.12902216835438088	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	93	0.12120264299956994	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	90	0.11729288032216445	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	86	0.11207986341895713	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	84	0.10947335496735348	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	83	0.10817010074155166	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	80	0.10426033806414617	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.001303254225801827	0.0	0.0	0.0
5	0.0	0.001303254225801827	0.0	0.0	0.0
6	0.0	0.001303254225801827	0.0	0.0	0.0
7	0.0	0.001303254225801827	0.0	0.0	0.0
8	0.0	0.001303254225801827	0.0	0.0	0.0
9	0.0	0.001303254225801827	0.0	0.0	0.0
10	0.0	0.001303254225801827	0.0	0.0	0.0
11	0.0	0.001303254225801827	0.0	0.0	0.0
12	0.0	0.001303254225801827	0.0	0.0	0.0
13	0.0	0.001303254225801827	0.0	0.0	0.0
14	0.0	0.001303254225801827	0.0	0.0	0.0
15	0.0	0.001303254225801827	0.0	0.0	0.0
16	0.0	0.001303254225801827	0.0	0.0	0.001303254225801827
17	0.0	0.001303254225801827	0.0	0.0	0.001303254225801827
18	0.0	0.001303254225801827	0.0	0.0	0.001303254225801827
19	0.0	0.001303254225801827	0.0	0.0	0.001303254225801827
20	0.0	0.001303254225801827	0.0	0.0	0.001303254225801827
21	0.0	0.001303254225801827	0.0	0.0	0.001303254225801827
22	0.0	0.001303254225801827	0.0	0.002606508451603654	0.001303254225801827
23	0.0	0.001303254225801827	0.0	0.002606508451603654	0.001303254225801827
24	0.0	0.001303254225801827	0.0	0.007819525354810963	0.001303254225801827
25	0.0	0.001303254225801827	0.0	0.007819525354810963	0.001303254225801827
26	0.0	0.001303254225801827	0.0	0.013032542258018271	0.001303254225801827
27	0.0	0.001303254225801827	0.0	0.037794372548252984	0.001303254225801827
28	0.0	0.001303254225801827	0.0	0.14075145638659733	0.001303254225801827
29	0.0	0.001303254225801827	0.0	0.2880191839022038	0.001303254225801827
30	0.0	0.001303254225801827	0.0	0.49784311425629796	0.001303254225801827
31	0.0	0.001303254225801827	0.0	1.1012498208025439	0.001303254225801827
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGAAAC	20	0.0018312484	37.0	25
AACATCT	20	0.0018312484	37.0	29
TATACAC	20	0.0018312484	37.0	37
TGAACCG	30	3.5685228E-4	30.833332	5
AGTGAGC	30	3.5685228E-4	30.833332	8
GGTATCA	105	0.0	29.95238	1
TACCCCG	25	0.005464815	29.6	5
CATCTAA	25	0.005464815	29.6	31
TGAAACA	25	0.005464815	29.6	26
ACAGGAA	25	0.005464815	29.6	28
TCTAAGT	25	0.005464815	29.6	33
GAAACAT	25	0.005464815	29.6	27
GAACCGT	25	0.005464815	29.6	6
ACATCTA	25	0.005464815	29.6	30
CACAATA	25	0.005464815	29.6	34
GGCGAAC	25	0.005464815	29.6	10
GGTGGAT	35	8.793833E-4	26.428572	10
TACCACG	65	2.6290654E-6	22.76923	4
GGATACC	65	2.6290654E-6	22.76923	1
AACTCCG	65	2.6290654E-6	22.76923	5
>>END_MODULE