FastQCFastQC Report
Fri 10 Feb 2017
ERR1632625.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632625.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16307
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT790.4844545287299933RNA PCR Primer, Index 41 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA620.3802048200159441No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA400.245293432268351No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT330.20236708162138958No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA330.20236708162138958No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA320.19623474581468078No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA320.19623474581468078No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA310.19010241000797204No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA310.19010241000797204No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA280.17170540258784572No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT280.17170540258784572No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC240.1471760593610106No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT240.1471760593610106No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA240.1471760593610106No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC240.1471760593610106No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC220.13491138774759306No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT220.13491138774759306No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA210.1287790519408843No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC210.1287790519408843No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC210.1287790519408843RNA PCR Primer, Index 41 (95% over 23bp)
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA210.1287790519408843No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC210.1287790519408843No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA210.1287790519408843No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA200.1226467161341755No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC200.1226467161341755No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC200.1226467161341755No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT200.1226467161341755No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA190.11651438032746672No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG190.11651438032746672No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT190.11651438032746672No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT180.11038204452075795No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT180.11038204452075795No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG180.11038204452075795No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC170.10424970871404918No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA170.10424970871404918No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT170.10424970871404918No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC170.10424970871404918No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT170.10424970871404918No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG170.10424970871404918No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC170.10424970871404918No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT170.10424970871404918No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTTA750.003878326214.837
GTCTCTT1100.003481224911.77272737
Produced by FastQC (version 0.11.5)