FastQCFastQC Report
Fri 10 Feb 2017
ERR1632624.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632624.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12702
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT390.30703826169107223RNA PCR Primer, Index 33 (95% over 22bp)
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA220.17320107069752794No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA210.16532829475673122No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG210.16532829475673122No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA200.1574555188159345No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA160.1259644150527476No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA140.11021886317115415No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA140.11021886317115415No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC130.10234608723035743No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA130.10234608723035743No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC130.10234608723035743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGTC303.404698E-430.83333236
Produced by FastQC (version 0.11.5)