##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632624.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12702
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95906156510786	34.0	33.0	34.0	31.0	34.0
2	33.08825381829633	34.0	33.0	34.0	31.0	34.0
3	33.16831994961424	34.0	33.0	34.0	31.0	34.0
4	36.459927570461346	37.0	37.0	37.0	35.0	37.0
5	36.4417414580381	37.0	37.0	37.0	35.0	37.0
6	36.50684931506849	37.0	37.0	37.0	35.0	37.0
7	36.49818926153362	37.0	37.0	37.0	35.0	37.0
8	36.46976854038734	37.0	37.0	37.0	35.0	37.0
9	38.261927255550304	39.0	39.0	39.0	37.0	39.0
10	38.288694693749015	39.0	39.0	39.0	37.0	39.0
11	38.35317272870414	39.0	39.0	39.0	37.0	39.0
12	38.32797984569359	39.0	39.0	39.0	37.0	39.0
13	38.34600850259802	39.0	39.0	39.0	37.0	39.0
14	39.8971815462132	41.0	40.0	41.0	38.0	41.0
15	39.888364037159505	41.0	40.0	41.0	38.0	41.0
16	39.83270351125807	41.0	40.0	41.0	38.0	41.0
17	39.805857345299955	41.0	40.0	41.0	38.0	41.0
18	39.820028341993385	41.0	40.0	41.0	38.0	41.0
19	39.86112423240434	41.0	40.0	41.0	38.0	41.0
20	39.85427491733585	41.0	40.0	41.0	38.0	41.0
21	39.8132577546843	41.0	40.0	41.0	38.0	41.0
22	39.76720201543064	41.0	40.0	41.0	38.0	41.0
23	39.73145961265942	41.0	40.0	41.0	38.0	41.0
24	39.71287986143914	41.0	40.0	41.0	38.0	41.0
25	39.64383561643836	41.0	40.0	41.0	37.0	41.0
26	39.573295544008815	41.0	40.0	41.0	37.0	41.0
27	39.48291607620847	41.0	40.0	41.0	37.0	41.0
28	39.458746654070225	41.0	39.0	41.0	37.0	41.0
29	39.395370807746815	41.0	39.0	41.0	37.0	41.0
30	39.389702409069436	41.0	39.0	41.0	36.0	41.0
31	39.32042198079043	41.0	39.0	41.0	36.0	41.0
32	39.21799716580066	41.0	39.0	41.0	36.0	41.0
33	39.16831994961424	41.0	39.0	41.0	35.0	41.0
34	39.13667139033223	41.0	39.0	41.0	35.0	41.0
35	39.02243741143127	41.0	39.0	41.0	35.0	41.0
36	38.95961265942371	41.0	39.0	41.0	35.0	41.0
37	38.94260746339159	40.0	39.0	41.0	35.0	41.0
38	38.844276491891044	40.0	39.0	41.0	35.0	41.0
39	38.818768697842856	40.0	39.0	41.0	35.0	41.0
40	38.68792316170682	40.0	38.0	41.0	35.0	41.0
41	38.6669815777043	40.0	38.0	41.0	35.0	41.0
42	38.63493937962526	40.0	38.0	41.0	35.0	41.0
43	37.96299795307826	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	1.0
22	2.0
23	3.0
24	10.0
25	18.0
26	19.0
27	28.0
28	32.0
29	51.0
30	60.0
31	70.0
32	134.0
33	164.0
34	186.0
35	324.0
36	494.0
37	949.0
38	2536.0
39	7620.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.772634230829794	16.643048338844277	12.761769800031491	26.82254763029444
2	21.53991497401984	19.193827743662414	32.105180286569045	27.1610769957487
3	21.862698787592507	19.784285939222173	28.86946937490159	29.483545898283737
4	15.81640686506062	15.501495827428752	32.01070697527948	36.67139033223115
5	16.918595496772163	32.7035112580696	33.40418831680051	16.973704928357737
6	36.002204377263425	32.97118564005668	14.753582113053062	16.27302786962683
7	30.396787907416154	28.05070067705873	19.532357109116674	22.02015430640844
8	27.26342308297906	31.530467642890887	20.445599118249095	20.76051015588096
9	27.85388127853881	13.139663045189733	17.099669343410486	41.90678633286097
10	20.06770587309085	22.8389230042513	29.404818138875765	27.688552983782085
11	38.545111006140765	19.406392694063925	18.87104393008975	23.17745236970556
12	23.5553456148638	23.452999527633445	26.326562746024244	26.665092111478504
13	34.17572035899858	17.81609195402299	20.713273500236184	27.294914186742247
14	24.681152574397732	19.319792158715163	22.453156983152258	33.54589828373484
15	29.633128641158873	23.712801133679736	19.988978113682883	26.665092111478504
16	26.59423712801134	24.5236970555818	21.736734372539757	27.14533144386711
17	26.9327664934656	23.618327822390174	21.335222799559126	28.113682884585106
18	25.854196189576445	21.335222799559126	23.712801133679736	29.097779877184692
19	28.042827901117935	23.405762871988664	22.957014643363248	25.59439458353015
20	29.420563690757362	20.79200125964415	23.382144544166273	26.405290505432216
21	28.30262950716423	22.272083136513935	21.49267831837506	27.932609037946783
22	27.97197291765076	22.78381357266572	21.3903322311447	27.85388127853881
23	27.554715792788535	22.46102975909306	22.342938119981106	27.6413163281373
24	28.38923004251299	21.870571563533304	21.909935443237284	27.830262950716424
25	29.026924893717528	22.177609825224373	22.004408754526846	26.791056526531253
26	27.987718469532357	22.957014643363248	22.531884742560226	26.523382144544165
27	27.96410014170997	21.878444339474097	22.775940796724925	27.381514722091012
28	26.775310974649663	23.00425129900803	22.193355377105966	28.027082349236345
29	27.271295858919853	23.374271768225476	21.862698787592507	27.491733585262164
30	27.21618642733428	22.642103605731382	23.090851834356794	27.05085813257755
31	27.609825224374113	21.980790426704456	22.752322468902538	27.657061880018897
32	25.413320736891826	22.917650763659267	23.0751062824752	28.593922216973706
33	26.326562746024244	21.89418989135569	24.074948826956387	27.704298535663675
34	27.121713116044717	22.169737049283576	23.39001732010707	27.318532514564637
35	26.531254920484965	23.027869626830423	23.673437253975752	26.767438198708867
36	26.334435521965045	21.26436781609195	24.476460399937018	27.924736262005982
37	26.224216658793893	21.75247992442135	24.342623208943472	27.680680207841284
38	25.618012911352544	21.406077783026294	25.389702409069436	27.586206896551722
39	25.618012911352544	20.059833097150054	26.279326090379467	28.042827901117935
40	24.137931034482758	20.516454101716263	28.601794992914503	26.743819870886476
41	23.421508423870257	19.92599590615651	28.609667768855296	28.042827901117935
42	22.051645410171627	20.6739096205322	29.766965832152415	27.507479137143758
43	21.6422610612502	21.067548417572034	29.082034325303102	28.20815619587467
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.0
8	2.0
9	3.0
10	1.5
11	0.0
12	0.0
13	3.0
14	6.0
15	9.0
16	12.0
17	13.0
18	14.0
19	14.0
20	19.5
21	25.0
22	21.0
23	17.0
24	19.5
25	22.0
26	22.0
27	30.0
28	38.0
29	53.0
30	68.0
31	76.5
32	85.0
33	85.0
34	108.0
35	131.0
36	157.0
37	183.0
38	257.0
39	331.0
40	331.0
41	391.5
42	452.0
43	476.0
44	500.0
45	646.5
46	793.0
47	793.0
48	936.0
49	1079.0
50	1219.0
51	1359.0
52	1436.5
53	1514.0
54	1514.0
55	1466.0
56	1418.0
57	1290.5
58	1163.0
59	1052.0
60	941.0
61	941.0
62	882.5
63	824.0
64	696.0
65	568.0
66	474.0
67	380.0
68	380.0
69	348.0
70	316.0
71	253.5
72	191.0
73	148.5
74	106.0
75	106.0
76	85.5
77	65.0
78	55.0
79	45.0
80	33.5
81	22.0
82	22.0
83	21.0
84	20.0
85	12.5
86	5.0
87	3.5
88	2.0
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	12702.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.60951031333649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.0439082278481	70.09132420091323
2	6.823575949367089	10.86443079829948
3	2.4030854430379747	5.739253660840812
4	1.0383702531645569	3.306565895134624
5	0.5537974683544303	2.204377263423083
6	0.3065664556962025	1.4643363249881909
7	0.26700949367088606	1.487954652810581
8	0.24723101265822786	1.5745551881593451
9	0.07911392405063292	0.5668398677373643
>10	0.23734177215189875	2.7003621476932764
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	39	0.30703826169107223	RNA PCR Primer, Index 33 (95% over 22bp)
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	22	0.17320107069752794	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	21	0.16532829475673122	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	21	0.16532829475673122	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	20	0.1574555188159345	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	16	0.1259644150527476	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	14	0.11021886317115415	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	14	0.11021886317115415	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	13	0.10234608723035743	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	13	0.10234608723035743	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	13	0.10234608723035743	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.007872775940796726	0.0
22	0.0	0.0	0.0	0.007872775940796726	0.0
23	0.0	0.0	0.0	0.007872775940796726	0.0
24	0.0	0.0	0.0	0.01574555188159345	0.0
25	0.0	0.0	0.0	0.01574555188159345	0.0
26	0.0	0.0	0.0	0.01574555188159345	0.0
27	0.0	0.0	0.0	0.04723665564478035	0.0
28	0.0	0.0	0.0	0.1574555188159345	0.0
29	0.0	0.0	0.0	0.33065658951346244	0.0
30	0.0	0.0	0.0	0.5904581955597543	0.0
31	0.0	0.0	0.0	1.1336797354747283	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTGTC	30	3.404698E-4	30.833332	36
>>END_MODULE