FastQCFastQC Report
Fri 10 Feb 2017
ERR1632623.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632623.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21716
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT920.4236507644133358TruSeq Adapter, Index 5 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA870.4006262663473936No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA650.2993184748572481No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT420.19340578375391415No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA400.1841959845275373No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA390.1795910849143489No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA370.170381285687972No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT360.16577638607478357No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT360.16577638607478357No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC360.16577638607478357No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA360.16577638607478357No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT340.1565665868484067No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC330.15196168723521827No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC320.14735678762202983No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC320.14735678762202983No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA310.1427518880088414No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT310.1427518880088414No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA310.1427518880088414No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC300.13814698839565298No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT290.13354208878246454No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT290.13354208878246454No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC280.12893718916927613Illumina Paired End PCR Primer 2 (95% over 23bp)
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG280.12893718916927613No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC280.12893718916927613No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG270.12433228955608767No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA260.11972738994289925No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG260.11972738994289925No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA260.11972738994289925No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG250.11512249032971081No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA250.11512249032971081No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC240.11051759071652237No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC220.10130779149014552No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC220.10130779149014552No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA220.10130779149014552No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC220.10130779149014552No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA358.5909764E-426.428571
GTATCAA400.001871132423.1252
CAACGCA608.8450714E-418.56
TATCAAC500.00682021418.4999983
GTCTCTT1200.00678794710.79166636
CTGTCTC2207.1635513E-610.09090937
Produced by FastQC (version 0.11.5)