FastQCFastQC Report
Fri 10 Feb 2017
ERR1632622.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632622.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107121
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2320.21657751514642323No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1870.17456894539819456No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1280.11949104283940591No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1200.11202285266194303No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1160.10828875757321162No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT1150.10735523380102875TruSeq Adapter, Index 10 (95% over 21bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1130.10548818625666302No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1100.10268761494011446No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTC250.00547439329.5999985
CTCGTAT250.00547439329.59999828
CGTCTTC250.00547439329.59999837
TCTCGTA250.00547439329.59999827
GGTATCA1150.028.9565221
TACGGCT358.816769E-426.4285730
ATACGGC358.816769E-426.4285729
GCGGTAA1100.025.22727223
CTAACTC1350.023.2962973
GCTAACT1350.023.2962972
AGCCGCG1300.022.7692319
CCGTGCC1400.022.4642859
TAACTCC1400.022.4642854
CGCGGTA1250.022.19999922
CGGTAAT1250.022.19999924
CTCCGTG1450.021.6896557
TCCGTGC1450.021.6896558
AACTCCG1450.021.6896555
GGCTAAC1450.021.6896551
GGTAATA1301.8189894E-1221.34615325
Produced by FastQC (version 0.11.5)