##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632622.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	107121
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42169135837044	33.0	31.0	34.0	31.0	34.0
2	32.60527814340792	34.0	31.0	34.0	31.0	34.0
3	32.69568992074383	34.0	31.0	34.0	31.0	34.0
4	36.23631220769037	37.0	37.0	37.0	35.0	37.0
5	36.00275389512794	37.0	35.0	37.0	35.0	37.0
6	36.00269788370161	37.0	35.0	37.0	35.0	37.0
7	36.006189262609574	37.0	35.0	37.0	35.0	37.0
8	35.95983980732069	37.0	35.0	37.0	35.0	37.0
9	37.639715835363745	39.0	37.0	39.0	35.0	39.0
10	37.59102323540669	39.0	37.0	39.0	35.0	39.0
11	37.68902456101045	39.0	37.0	39.0	35.0	39.0
12	37.62106403039554	39.0	37.0	39.0	35.0	39.0
13	37.669775300828036	39.0	37.0	39.0	35.0	39.0
14	38.984923591079244	40.0	38.0	41.0	36.0	41.0
15	38.93895688053696	40.0	38.0	41.0	36.0	41.0
16	38.906946350388814	40.0	38.0	41.0	36.0	41.0
17	38.88235733422952	40.0	38.0	41.0	36.0	41.0
18	38.9006544001643	40.0	38.0	41.0	36.0	41.0
19	38.91091382642059	40.0	38.0	41.0	36.0	41.0
20	38.86136238459312	40.0	38.0	41.0	36.0	41.0
21	38.816375874011634	40.0	38.0	41.0	35.0	41.0
22	38.76941962827083	40.0	38.0	41.0	35.0	41.0
23	38.73272280878633	40.0	38.0	41.0	35.0	41.0
24	38.690135454299345	40.0	38.0	41.0	35.0	41.0
25	38.63066065477357	40.0	38.0	41.0	35.0	41.0
26	38.51748023263412	40.0	38.0	41.0	35.0	41.0
27	38.41617423287684	40.0	38.0	41.0	34.0	41.0
28	38.34547847760943	40.0	38.0	41.0	34.0	41.0
29	38.306942616293725	40.0	38.0	41.0	34.0	41.0
30	38.25193006039899	40.0	38.0	41.0	34.0	41.0
31	38.19637606071639	40.0	38.0	41.0	34.0	41.0
32	38.12903165579112	40.0	38.0	41.0	34.0	41.0
33	38.07088246002184	40.0	38.0	41.0	34.0	41.0
34	38.0283511169612	40.0	38.0	41.0	33.0	41.0
35	37.924431250641796	40.0	37.0	41.0	33.0	41.0
36	37.8569468171507	40.0	37.0	41.0	33.0	41.0
37	37.814387468376886	40.0	37.0	41.0	33.0	41.0
38	37.739817589454915	40.0	37.0	41.0	33.0	41.0
39	37.641274820063295	40.0	37.0	41.0	33.0	41.0
40	37.54799712474678	39.0	37.0	41.0	33.0	41.0
41	37.50529774740714	39.0	36.0	41.0	33.0	41.0
42	37.43556352162508	39.0	36.0	41.0	33.0	41.0
43	36.59143398586645	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	0.0
18	4.0
19	4.0
20	14.0
21	20.0
22	48.0
23	68.0
24	101.0
25	183.0
26	234.0
27	328.0
28	483.0
29	651.0
30	905.0
31	1233.0
32	1651.0
33	2316.0
34	3524.0
35	5498.0
36	9329.0
37	18223.0
38	33958.0
39	28344.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.80953314476153	16.82396542227948	12.517620261199951	27.84888117175904
2	21.89953417163768	19.280999990664764	31.4998926447662	27.319573192931358
3	21.668020276136332	19.959671773041702	28.198019062555428	30.17428888826654
4	16.391743915758813	15.522633283856575	32.93285163506688	35.15277116531772
5	17.792963097805288	32.82362935372149	32.616387076296895	16.767020472176323
6	35.76142866478095	33.069146105805586	14.599378273167726	16.57004695624574
7	31.01446028323111	27.063787679353258	19.196982851168304	22.72476918624733
8	27.73872536664146	30.82028733861708	19.183913518357745	22.257073776383717
9	27.675245750133026	12.697790349231244	17.27765797556035	42.349305925075384
10	19.52184912388794	23.14298783618525	29.8335527114198	27.501610328507013
11	40.17232848834495	19.16244247159754	18.297999458556212	22.367229581501295
12	23.5976139132383	23.356764780015123	26.223616284388683	26.822005022357892
13	35.16210640303956	17.655735103294408	20.10343443395786	27.078724059708183
14	24.389242072049363	19.399557509731984	22.284145965777018	33.92705445244163
15	29.57776719784169	23.801122095574165	19.6329384527777	26.988172253806443
16	26.691311694252295	23.47998991794326	21.83138693626833	27.99731145153611
17	27.09272691629092	23.255010688847193	21.54386161443601	28.108400780425875
18	26.74919016812763	21.434639333090615	23.196198691199672	28.61997180758208
19	28.50794895492014	22.187059493470002	22.53899795558294	26.76599359602692
20	29.755136714556436	21.06309687176184	22.23280215830696	26.94896425537476
21	27.759262889629483	21.919138170853522	21.74550274922751	28.576096190289483
22	28.703988947078535	22.877867084885317	20.979079732265383	27.439064235770765
23	28.753465707004228	22.014357595616172	21.724031702467304	27.5081449949123
24	28.60596895099934	21.367425621493453	21.319815909112126	28.706789518395087
25	28.69091961426798	21.806181794419395	22.34295796342454	27.15994062788809
26	28.344582294788133	22.407371103705156	22.061033784225316	27.187012817281392
27	27.61736727625769	21.874329029788743	23.131785550919055	27.376518143034513
28	27.02084558583284	22.829323848731807	21.989152453767236	28.160678111668112
29	27.621101371346423	23.160724787856722	21.82111817477432	27.397055666022535
30	26.977903492312432	21.97701664472886	23.550937724629158	27.494142138329554
31	27.82087545859355	21.935941598752812	22.280411870688287	27.96277107196535
32	25.941692105189457	21.796846556697567	22.854528990580743	29.406932347532226
33	26.320702756695702	21.67735551385816	23.947685327806873	28.054256401639265
34	26.707181598379403	21.390763715798023	24.072777513279377	27.8292771725432
35	26.287095900897118	22.52219452768365	24.078378655912474	27.112330915506767
36	26.597025793261825	21.219928865488562	24.710374249680267	27.472671091569346
37	26.639967886782234	20.509517274857405	24.752382819428497	28.09813201893186
38	25.423586411627973	20.232260714519096	26.152668477702782	28.191484396150145
39	25.01750357072843	19.392089319554522	27.43439661690985	28.156010492807198
40	24.496597305850393	20.091298624919485	28.445402862183883	26.96670120704624
41	23.187796977250024	19.56385769363617	29.156747976587223	28.09159735252658
42	21.475714379066662	20.615005461114066	30.445010782199567	27.4642693776197
43	21.123775916953726	20.719560123598548	29.584301864246974	28.572362095200752
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	1.0
6	0.5
7	0.0
8	4.0
9	8.0
10	7.0
11	6.0
12	6.0
13	19.0
14	32.0
15	52.0
16	72.0
17	74.0
18	76.0
19	76.0
20	97.5
21	119.0
22	122.5
23	126.0
24	145.0
25	164.0
26	164.0
27	208.0
28	252.0
29	326.5
30	401.0
31	538.5
32	676.0
33	676.0
34	974.0
35	1272.0
36	1514.0
37	1756.0
38	2127.5
39	2499.0
40	2499.0
41	3070.5
42	3642.0
43	4282.0
44	4922.0
45	5971.5
46	7021.0
47	7021.0
48	7994.0
49	8967.0
50	9840.0
51	10713.0
52	11119.5
53	11526.0
54	11526.0
55	11184.5
56	10843.0
57	10386.0
58	9929.0
59	9430.5
60	8932.0
61	8932.0
62	8161.5
63	7391.0
64	6236.0
65	5081.0
66	4364.5
67	3648.0
68	3648.0
69	3091.0
70	2534.0
71	2117.5
72	1701.0
73	1380.5
74	1060.0
75	1060.0
76	849.0
77	638.0
78	521.5
79	405.0
80	354.0
81	303.0
82	303.0
83	242.5
84	182.0
85	137.0
86	92.0
87	87.0
88	82.0
89	82.0
90	57.5
91	33.0
92	21.5
93	10.0
94	7.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	107121.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.30013722799451
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.02269818818617	61.69285200847639
2	5.50414225095979	7.62875626627832
3	1.885902876001886	3.9207998431680062
4	0.9927931568667071	2.752028080395067
5	0.5523001279719808	1.9137237329748602
6	0.3717922812689432	1.5459153667348138
7	0.2977032397117263	1.4441612755668825
8	0.19397858153162253	1.0754193855546532
9	0.1333602748029905	0.831769681014927
>10	0.9671987606923957	12.718327872219264
>50	0.06196538021149054	3.040486925999571
>100	0.01616488179430188	1.4357595616172365
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	232	0.21657751514642323	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	187	0.17456894539819456	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	128	0.11949104283940591	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	120	0.11202285266194303	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	116	0.10828875757321162	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	115	0.10735523380102875	TruSeq Adapter, Index 10 (95% over 21bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	113	0.10548818625666302	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	110	0.10268761494011446	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	9.335237721828587E-4	0.0
17	0.0	0.0	0.0	0.0018670475443657174	0.0
18	0.0	0.0	0.0	0.0018670475443657174	0.0
19	0.0	0.0	0.0	0.002800571316548576	0.0
20	0.0	0.0	0.0	0.002800571316548576	0.0
21	0.0	0.0	0.0	0.005601142633097152	0.0
22	0.0	0.0	0.0	0.009335237721828586	0.0
23	0.0	0.0	0.0	0.009335237721828586	0.0
24	9.335237721828587E-4	0.0	0.0	0.013069332810560021	0.0
25	9.335237721828587E-4	0.0	0.0	0.014936380354925739	0.0
26	9.335237721828587E-4	0.0	0.0	0.01960399921584003	0.0
27	9.335237721828587E-4	0.0	0.0	0.05507790255878866	0.0
28	9.335237721828587E-4	0.0	0.0	0.19137237329748602	0.0
29	9.335237721828587E-4	0.0	0.0	0.3883458892280692	0.0
30	9.335237721828587E-4	0.0	0.0	0.6628018782498296	0.0
31	9.335237721828587E-4	0.0	0.0	1.3862828016915452	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCTC	25	0.005474393	29.599998	5
CTCGTAT	25	0.005474393	29.599998	28
CGTCTTC	25	0.005474393	29.599998	37
TCTCGTA	25	0.005474393	29.599998	27
GGTATCA	115	0.0	28.956522	1
TACGGCT	35	8.816769E-4	26.42857	30
ATACGGC	35	8.816769E-4	26.42857	29
GCGGTAA	110	0.0	25.227272	23
CTAACTC	135	0.0	23.296297	3
GCTAACT	135	0.0	23.296297	2
AGCCGCG	130	0.0	22.76923	19
CCGTGCC	140	0.0	22.464285	9
TAACTCC	140	0.0	22.464285	4
CGCGGTA	125	0.0	22.199999	22
CGGTAAT	125	0.0	22.199999	24
CTCCGTG	145	0.0	21.689655	7
TCCGTGC	145	0.0	21.689655	8
AACTCCG	145	0.0	21.689655	5
GGCTAAC	145	0.0	21.689655	1
GGTAATA	130	1.8189894E-12	21.346153	25
>>END_MODULE