FastQCFastQC Report
Fri 10 Feb 2017
ERR1632621.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632621.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70882
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3890.5487994131091108No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3510.49518918766400494No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2670.3766823735221918No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2480.3498772607996388No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2410.34000169295448773No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2300.324482943483536No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2260.3188397618577354No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2250.3174289664512852No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2240.3160181710448351No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2170.306142603199684No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2100.2962670353545329No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2080.29344544454163257No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1970.27792669507068085No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1840.2595863547868288No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1760.24829999153522758No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1730.2440676053158771No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1710.24124601450297678No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT1680.23701362828362632TruSeq Adapter, Index 7 (95% over 21bp)
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1660.234192037470726No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1610.22713806043847523No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1600.22572726503202506No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1550.21867328799977429No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1540.21726249259332414No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1460.20597612934172288No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1450.2045653339352727No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1430.20174374312237237No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1420.20033294771592222No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1410.1989221523094721No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1380.19468976609012162No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1380.19468976609012162No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1340.189046584464321No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1330.18763578905787084No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1300.18340340283852036No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1270.17917101661916987No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1250.17634942580626958No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1250.17634942580626958No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1220.17211703958691912No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1200.16929544877401878No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1190.16788465336756864No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1170.16506306255466832No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1170.16506306255466832No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1130.1594198809288677No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1110.15659829011596738No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1090.15377669930306706No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1090.15377669930306706No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1080.15236590389661692No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1080.15236590389661692No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT1070.15095510849016675No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1060.1495443130837166No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1040.14672272227081629No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1040.14672272227081629No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1030.14531192686436611No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1030.14531192686436611No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1020.14390113145791597No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1020.14390113145791597No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1010.14249033605146583No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC990.13966874523856548No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG980.13825794983211537No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG980.13825794983211537No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA960.13543635901921502No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC950.13402556361276488No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA940.13261476820631474No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT920.1297931773934144No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA920.1297931773934144No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT910.12838238198696425No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT900.1269715865805141No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG870.12273920036116363No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT860.12132840495471346No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC860.12132840495471346No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA850.1199176095482633No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG840.11850681414181316No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC840.11850681414181316No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA840.11850681414181316No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT830.117096018735363No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA820.11568522332891283No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC810.11427442792246269No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT800.11286363251601253No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA800.11286363251601253No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC800.11286363251601253No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC800.11286363251601253No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG800.11286363251601253No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC790.11145283710956237No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT790.11145283710956237No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA790.11145283710956237No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG790.11145283710956237No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA790.11145283710956237No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC780.1100420417031122No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG780.1100420417031122No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG770.10863124629666207No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG770.10863124629666207No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA760.1072204508902119No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG760.1072204508902119No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT760.1072204508902119No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT740.1043988600773116No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC730.10298806467086144No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT730.10298806467086144No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG730.10298806467086144No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA730.10298806467086144No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG720.10157726926441127No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC720.10157726926441127No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC710.10016647385796111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCC200.001830304537.012
AGTAGTT200.001830304537.019
CAGTAGT200.001830304537.018
AGCTACC200.001830304537.06
ACCGTTT200.001830304537.010
TAGCTAC200.001830304537.05
GTATTAG200.001830304537.01
CCGTTTC200.001830304537.011
TCCAGTA200.001830304537.016
GCTACCG200.001830304537.07
TATTAGC200.001830304537.02
CTACCGT200.001830304537.08
ATCCCCC250.00546203229.626
TTATCCC250.00546203229.624
CTCCATC250.00546203229.632
TAGTTAT250.00546203229.621
CCTCCAT250.00546203229.631
TACCGTT250.00546203229.69
TCCATCA250.00546203229.633
CCCCTCC250.00546203229.629
Produced by FastQC (version 0.11.5)