FastQCFastQC Report
Fri 10 Feb 2017
ERR1632620.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632620.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14165
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT420.2965054712319096RNA PCR Primer, Index 39 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA400.2823861630780092No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC310.2188492763854571No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA270.1906106600776562No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA250.17649135192375573No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG240.1694316978468055No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC230.16237204376985526No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC220.15531238969290506No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT200.1411930815390046No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG200.1411930815390046No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA200.1411930815390046No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC200.1411930815390046No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG200.1411930815390046No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC190.13413342746205437No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA190.13413342746205437No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG180.12707377338510412No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC180.12707377338510412No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT170.12001411930815391No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC170.12001411930815391No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT170.12001411930815391No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC170.12001411930815391No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA170.12001411930815391No Hit
GCTTTGCGTTCTCGGGGTGCCATAGCCGGGTTTGAGTTAGCAG170.12001411930815391No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT160.11295446523120367No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA160.11295446523120367No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT160.11295446523120367No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT150.10589481115425345No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG150.10589481115425345No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC150.10589481115425345No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC150.10589481115425345No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA150.10589481115425345No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT150.10589481115425345No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC150.10589481115425345No Hit
GTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCC150.10589481115425345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATA352.2271128E-531.71428537
CTCTTAT405.542311E-527.7536
CTTTAGT450.003644954420.5555552
TTTAGTG450.003644954420.5555553
TCTTTAG450.003644954420.5555551
TCTCTTA608.6373376E-418.535
GTCTCTT800.005902683413.87534
Produced by FastQC (version 0.11.5)