##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632620.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14165
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.96780797740911	34.0	33.0	34.0	31.0	34.0
2	33.0944581715496	34.0	33.0	34.0	31.0	34.0
3	33.20232968584539	34.0	33.0	34.0	31.0	34.0
4	36.49763501588422	37.0	37.0	37.0	35.0	37.0
5	36.46953759265796	37.0	37.0	37.0	35.0	37.0
6	36.51302506177198	37.0	37.0	37.0	35.0	37.0
7	36.47624426403106	37.0	37.0	37.0	35.0	37.0
8	36.457183198023294	37.0	37.0	37.0	35.0	37.0
9	38.26332509707024	39.0	39.0	39.0	37.0	39.0
10	38.30067066713731	39.0	39.0	39.0	37.0	39.0
11	38.380515354747615	39.0	39.0	39.0	37.0	39.0
12	38.26953759265796	39.0	39.0	39.0	37.0	39.0
13	38.311260148252735	39.0	39.0	39.0	37.0	39.0
14	39.864948817507944	41.0	40.0	41.0	38.0	41.0
15	39.84673490998941	41.0	40.0	41.0	38.0	41.0
16	39.83939286974938	41.0	40.0	41.0	38.0	41.0
17	39.80868337451465	41.0	40.0	41.0	38.0	41.0
18	39.786021884927635	41.0	40.0	41.0	38.0	41.0
19	39.77677373808683	41.0	40.0	41.0	38.0	41.0
20	39.74994705259442	41.0	40.0	41.0	38.0	41.0
21	39.7618072714437	41.0	40.0	41.0	38.0	41.0
22	39.71768443346276	41.0	40.0	41.0	38.0	41.0
23	39.693328626897284	41.0	40.0	41.0	38.0	41.0
24	39.64122837980939	41.0	40.0	41.0	37.0	41.0
25	39.64729968231557	41.0	40.0	41.0	37.0	41.0
26	39.52601482527356	41.0	40.0	41.0	37.0	41.0
27	39.40148252735616	41.0	39.0	41.0	37.0	41.0
28	39.395340628309214	41.0	39.0	41.0	37.0	41.0
29	39.33851041298976	41.0	39.0	41.0	37.0	41.0
30	39.31281327214966	41.0	39.0	41.0	36.0	41.0
31	39.20543593363925	41.0	39.0	41.0	36.0	41.0
32	39.22082597952701	41.0	39.0	41.0	36.0	41.0
33	39.11733145075891	41.0	39.0	41.0	35.0	41.0
34	39.08570420049418	41.0	39.0	41.0	35.0	41.0
35	39.03558065654783	40.0	39.0	41.0	35.0	41.0
36	38.952488528062126	40.0	39.0	41.0	35.0	41.0
37	38.866431344864104	40.0	39.0	41.0	35.0	41.0
38	38.81835510060007	40.0	38.0	41.0	35.0	41.0
39	38.67920931874338	40.0	38.0	41.0	35.0	41.0
40	38.623649841157786	40.0	38.0	41.0	35.0	41.0
41	38.56872573243911	40.0	38.0	41.0	35.0	41.0
42	38.49064595834804	40.0	38.0	41.0	35.0	41.0
43	37.71182492057889	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	4.0
23	5.0
24	9.0
25	8.0
26	16.0
27	26.0
28	44.0
29	46.0
30	65.0
31	106.0
32	120.0
33	174.0
34	238.0
35	377.0
36	596.0
37	1256.0
38	3057.0
39	8018.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.016943169784675	16.081891987292625	12.63678079774091	27.26438404518179
2	21.920225908930462	19.12460289445817	31.944934698199788	27.010236498411576
3	22.230850688316274	18.74338157430286	28.344511118955175	30.681256618425696
4	15.884221673138017	14.698199788210378	33.46276032474409	35.95481821390752
5	17.966819625838333	32.650900105894806	31.69784680550653	17.684433462760325
6	36.08189198729262	32.51676667843276	15.354747617366748	16.04659371690787
7	30.977762089657606	26.981997882103776	19.145781856689023	22.894458171549594
8	28.386869043416873	29.62230850688316	20.395340628309214	21.595481821390752
9	28.090363572184962	12.559124602894459	16.681962583833393	42.66854924108719
10	19.06106600776562	23.233321567243205	29.594069890575366	28.11154253441581
11	40.55065301800212	18.348040945993645	18.10095305330039	23.00035298270385
12	22.259089304624073	22.717966819625836	27.15142957995058	27.871514295799503
13	35.94069890575362	16.491351923755737	20.303565125308857	27.26438404518179
14	25.49947052594423	19.759971761383692	21.91316625485351	32.827391457818564
15	29.170490645958346	23.812213201553124	20.021178962230852	26.996117190257678
16	28.168019767031417	22.20967172608542	21.673138016237203	27.949170490645958
17	27.920931874338155	22.852100247087893	21.150723614542887	28.076244264031065
18	26.240734204024	20.529474055771267	22.901517825626545	30.328273914578187
19	29.855277091422522	21.807271443699257	21.94846452523826	26.388986939639956
20	29.84821743734557	20.28944581715496	22.654429932933287	27.207906812566186
21	27.695022943875752	21.143663960465936	21.31309565831274	29.84821743734557
22	28.83868690434169	21.320155312389694	21.666078362160253	28.175079421108368
23	28.68337451464878	21.22132015531239	21.489587010236498	28.60571831980233
24	27.659724673491	21.440169431697846	21.91316625485351	28.986939639957644
25	28.923402753265087	21.447229085774797	21.94846452523826	27.680903635721847
26	29.078715142957996	22.42146134839393	20.536533709848218	27.963289798799856
27	28.175079421108368	21.418990469466998	22.287327920931872	28.118602188492765
28	26.97493822802683	22.37910342393223	21.574302859159904	29.071655488881042
29	27.850335333568655	23.289798799858808	21.736674902929757	27.12319096364278
30	28.633956936110135	20.833039181080125	22.569714084009885	27.963289798799856
31	27.659724673491	21.82139075185316	21.517825626544298	29.00105894811154
32	26.784327567949173	21.743734557006704	22.74620543593364	28.725732439110484
33	26.59371690787151	21.094246381927288	24.278150370631842	28.033886339569364
34	26.953759265795973	21.91316625485351	23.0921284857042	28.04094599364631
35	27.292622661489585	22.223791034239323	22.50617719731733	27.97740910695376
36	26.629015178256267	21.680197670314154	22.513236851394282	29.1775503000353
37	25.527709142252032	21.990822449699966	24.94881750794211	27.532650900105892
38	26.318390398870456	20.36710201200141	24.28521002470879	29.029297564419345
39	25.61242499117543	20.543593363925165	26.339569361101304	27.504412283798096
40	25.90187080833039	20.162372043769857	27.320861277797388	26.614895870102366
41	24.165195905400637	19.936463113307447	26.925520649488178	28.97282033180374
42	22.845040593010943	20.642428521002472	29.212848570420046	27.299682315566535
43	22.393222732086127	20.755382986233677	27.278503353335687	29.57289092834451
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	1.5
4	3.0
5	3.0
6	1.5
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	2.0
14	3.0
15	7.0
16	11.0
17	10.5
18	10.0
19	10.0
20	15.5
21	21.0
22	15.5
23	10.0
24	21.0
25	32.0
26	32.0
27	26.5
28	21.0
29	36.0
30	51.0
31	62.0
32	73.0
33	73.0
34	129.0
35	185.0
36	190.0
37	195.0
38	219.5
39	244.0
40	244.0
41	298.5
42	353.0
43	447.5
44	542.0
45	702.0
46	862.0
47	862.0
48	953.0
49	1044.0
50	1217.5
51	1391.0
52	1486.5
53	1582.0
54	1582.0
55	1544.5
56	1507.0
57	1441.5
58	1376.0
59	1366.0
60	1356.0
61	1356.0
62	1236.5
63	1117.0
64	853.0
65	589.0
66	536.0
67	483.0
68	483.0
69	430.0
70	377.0
71	362.5
72	348.0
73	289.0
74	230.0
75	230.0
76	147.5
77	65.0
78	49.5
79	34.0
80	28.0
81	22.0
82	22.0
83	18.5
84	15.0
85	10.5
86	6.0
87	5.0
88	4.0
89	4.0
90	2.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	14165.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.8789269325803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.57370517928287	61.36251323685139
2	6.424302788844622	9.106953759265796
3	2.460159362549801	5.23120367102012
4	1.245019920318725	3.5298270384751147
5	0.7768924302788844	2.7532650900105895
6	0.5876494023904383	2.499117543240381
7	0.3386454183266932	1.6801976703141546
8	0.3386454183266932	1.9202259089304625
9	0.24900398406374502	1.5884221673138015
>10	1.0059760956175299	10.328273914578185
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	42	0.2965054712319096	RNA PCR Primer, Index 39 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	40	0.2823861630780092	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	31	0.2188492763854571	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	27	0.1906106600776562	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	25	0.17649135192375573	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	24	0.1694316978468055	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	23	0.16237204376985526	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	22	0.15531238969290506	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	20	0.1411930815390046	No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG	20	0.1411930815390046	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	20	0.1411930815390046	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	20	0.1411930815390046	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	20	0.1411930815390046	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	19	0.13413342746205437	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	19	0.13413342746205437	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	18	0.12707377338510412	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	18	0.12707377338510412	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	17	0.12001411930815391	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	17	0.12001411930815391	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	17	0.12001411930815391	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	17	0.12001411930815391	No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA	17	0.12001411930815391	No Hit
GCTTTGCGTTCTCGGGGTGCCATAGCCGGGTTTGAGTTAGCAG	17	0.12001411930815391	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	16	0.11295446523120367	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	16	0.11295446523120367	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	16	0.11295446523120367	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	15	0.10589481115425345	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	15	0.10589481115425345	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	15	0.10589481115425345	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	15	0.10589481115425345	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	15	0.10589481115425345	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	15	0.10589481115425345	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	15	0.10589481115425345	No Hit
GTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCC	15	0.10589481115425345	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0070596540769502295	0.0
24	0.0	0.0	0.0	0.0070596540769502295	0.0
25	0.0	0.0	0.0	0.0070596540769502295	0.0
26	0.0	0.0	0.0	0.0070596540769502295	0.0
27	0.0	0.0	0.0	0.03529827038475115	0.0
28	0.0	0.0	0.0	0.13413342746205437	0.0
29	0.0	0.0	0.0	0.26826685492410873	0.0
30	0.0	0.0	0.0	0.522414401694317	0.0
31	0.0	0.0	0.0	1.0307094952347335	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTATA	35	2.2271128E-5	31.714285	37
CTCTTAT	40	5.542311E-5	27.75	36
CTTTAGT	45	0.0036449544	20.555555	2
TTTAGTG	45	0.0036449544	20.555555	3
TCTTTAG	45	0.0036449544	20.555555	1
TCTCTTA	60	8.6373376E-4	18.5	35
GTCTCTT	80	0.0059026834	13.875	34
>>END_MODULE