FastQCFastQC Report
Fri 10 Feb 2017
ERR1632619.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632619.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48772
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2440.5002870499466907No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT1760.36086279012548184RNA PCR Primer, Index 47 (95% over 22bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1650.33830886574263924No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1640.3362585089805626No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1320.27064709259411135No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1320.27064709259411135No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1250.25629459525957515No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1210.24809316821126876No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1130.23169031411465596No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1120.22963995735257933No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1120.22963995735257933No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1120.22963995735257933No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1110.22758960059050276No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1050.21528746001804314No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1020.2091363897318133No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1010.20708603296973674No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1000.2050356762076601No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA990.20298531944558354No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC980.2009349626835069No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC980.2009349626835069No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC970.19888460592143034No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT940.19273353563520051No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC940.19273353563520051No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG860.17633068153858772No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC840.17222996801443452No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA830.17017961125235792No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC830.17017961125235792No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC800.1640285409661281No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA800.1640285409661281No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC770.1578774706798983No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA720.1476256868695153No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC710.1455753301074387No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT710.1455753301074387No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC700.1435249733453621No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA690.1414746165832855No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC660.13532354629705567No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG660.13532354629705567No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC650.13327318953497908No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC650.13327318953497908No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT640.13122283277290248No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA640.13122283277290248No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC640.13122283277290248No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT630.12917247601082588No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA600.12302140572459609No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG600.12302140572459609No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA590.12097104896251946No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA580.11892069220044287No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG580.11892069220044287No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT570.11687033543836627No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG570.11687033543836627No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC570.11687033543836627No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT560.11481997867628967No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT550.11276962191421308No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT550.11276962191421308No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG540.11071926515213648No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA540.11071926515213648No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG540.11071926515213648No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA540.11071926515213648No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC530.10866890839005988No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG520.10661855162798328No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT520.10661855162798328No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC520.10661855162798328No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA520.10661855162798328No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC510.10456819486590666No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC510.10456819486590666No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT500.10251783810383006No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA500.10251783810383006No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT500.10251783810383006No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC490.10046748134175346No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC490.10046748134175346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC250.005445506429.637
ACGGTAC250.005445506429.63
GCGGTAA905.456968E-1226.72222123
CGCGGTA905.456968E-1226.72222122
ACGCTTG358.74765E-426.4285725
AACTCCG959.094947E-1225.3157865
TAATACG959.094947E-1225.31578627
TCCGTGC959.094947E-1225.3157868
CTCCGTG959.094947E-1225.3157867
CGGTAAT959.094947E-1225.31578624
GGTAATA959.094947E-1225.31578625
GGCTAAC901.3278623E-1024.6666661
TAACTCC1001.8189894E-1124.0500014
CTAACTC1001.8189894E-1124.0500013
GCTAACT1001.8189894E-1124.0500012
CCGCGGT1001.8189894E-1124.05000121
GGTATCA1101.8189894E-1223.5454541
AACGCTT400.001904928723.12500224
AGCGTAC400.001904928723.1250021
TCCGATT400.001904928723.12500217
Produced by FastQC (version 0.11.5)