##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632619.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	48772
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21852702370212	33.0	31.0	34.0	31.0	34.0
2	32.38095628639383	34.0	31.0	34.0	31.0	34.0
3	32.476338882965635	34.0	31.0	34.0	31.0	34.0
4	36.08396210940704	37.0	35.0	37.0	35.0	37.0
5	35.75075863200197	37.0	35.0	37.0	35.0	37.0
6	35.7468424505864	37.0	35.0	37.0	35.0	37.0
7	35.75539243828426	37.0	35.0	37.0	35.0	37.0
8	35.70056589846633	37.0	35.0	37.0	33.0	37.0
9	37.31290494546051	39.0	37.0	39.0	34.0	39.0
10	37.24715000410071	39.0	37.0	39.0	34.0	39.0
11	37.343762814729764	39.0	37.0	39.0	35.0	39.0
12	37.25654063807102	39.0	37.0	39.0	34.0	39.0
13	37.33861641925695	39.0	37.0	39.0	35.0	39.0
14	38.5230050028705	40.0	38.0	41.0	35.0	41.0
15	38.505987041745264	40.0	38.0	41.0	35.0	41.0
16	38.4513245304683	40.0	38.0	41.0	35.0	41.0
17	38.434081030099236	40.0	38.0	41.0	34.0	41.0
18	38.45956696465185	40.0	38.0	41.0	35.0	41.0
19	38.45478963339621	40.0	38.0	41.0	34.0	41.0
20	38.462150414172065	40.0	38.0	41.0	34.0	41.0
21	38.38046420077094	40.0	38.0	41.0	34.0	41.0
22	38.330722545722956	40.0	38.0	41.0	34.0	41.0
23	38.28292872959895	40.0	38.0	41.0	34.0	41.0
24	38.244874108094805	40.0	38.0	41.0	34.0	41.0
25	38.1948043959649	40.0	38.0	41.0	34.0	41.0
26	38.04381612400557	40.0	38.0	41.0	34.0	41.0
27	37.84431641105552	40.0	37.0	41.0	33.0	41.0
28	37.82949233166571	40.0	37.0	41.0	33.0	41.0
29	37.77519888460592	40.0	37.0	41.0	33.0	41.0
30	37.71176084638727	39.0	37.0	41.0	33.0	41.0
31	37.640039366849834	39.0	37.0	41.0	33.0	41.0
32	37.56796932666284	39.0	37.0	40.0	33.0	41.0
33	37.45825473632412	39.0	37.0	40.0	32.0	41.0
34	37.42883211678832	39.0	37.0	40.0	32.0	41.0
35	37.31388911670631	39.0	36.0	40.0	32.0	41.0
36	37.29955712293939	39.0	36.0	40.0	32.0	41.0
37	37.18436808004593	39.0	36.0	40.0	32.0	41.0
38	37.09698187484622	39.0	36.0	40.0	32.0	41.0
39	36.959074879028954	39.0	36.0	40.0	31.0	41.0
40	36.870355941933894	39.0	35.0	40.0	31.0	41.0
41	36.836238005412945	39.0	35.0	40.0	31.0	41.0
42	36.731731321249896	38.0	35.0	40.0	31.0	41.0
43	35.89559583367506	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	2.0
20	8.0
21	17.0
22	22.0
23	43.0
24	66.0
25	105.0
26	154.0
27	197.0
28	296.0
29	393.0
30	533.0
31	690.0
32	1024.0
33	1442.0
34	2235.0
35	3482.0
36	5792.0
37	9479.0
38	13471.0
39	9317.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.37472320183712	16.56073156729271	13.331419667022063	27.73312556384811
2	22.590830804559996	20.255474452554743	30.443697203313373	26.709997539571884
3	22.174608381858445	19.15443287131961	29.55384236857213	29.117116378249815
4	15.888214549331584	16.700155827113917	34.97703600426475	32.43459361928976
5	17.40957926679242	31.915853358484377	32.92462888542607	17.749938489297136
6	31.23103419995079	34.72279176576724	16.482818010333798	17.563356023948167
7	28.90182891823177	27.743377347658495	19.291806774378742	24.062986959730992
8	26.49471007955384	29.44107274665792	21.58000492085623	22.48421225293201
9	28.210858689411957	12.127860247683095	17.323464282785206	42.33781678011974
10	20.388747642089726	21.889608791929795	28.151398343311733	29.570245222668746
11	39.908144017058966	19.084720741409004	18.077995571229394	22.929139670302632
12	21.497990650373165	23.890756991716557	26.943738210448622	27.66751414746166
13	37.15861559911425	17.161486098581154	19.490691380300174	26.189206922004427
14	24.208562289838433	20.263675879603053	23.10547035184122	32.4222914787173
15	30.7307471500041	23.55859919626015	19.554252439924547	26.156401213811204
16	24.60223078815714	23.91946198638563	22.68924792913967	28.78905929631756
17	27.4009677683917	23.659066677601903	21.026408595095546	27.913556958910853
18	26.85557286967932	19.945870581481177	22.510866890839008	30.687689658000494
19	28.253916181415565	22.642089723611907	22.59698187484622	26.507012220126303
20	31.23103419995079	19.144181087509228	22.232018371196588	27.392766341343393
21	28.37283687361601	21.276552120068892	21.063315016812926	29.28729598950217
22	28.34413187894694	22.607233658656607	20.634790453538916	28.41384400885754
23	29.34470597884032	21.231444271303207	21.438530304272945	27.985319445583535
24	28.446649717050764	20.470761912572787	22.041335192323462	29.041253178052983
25	29.527187730665133	21.59640777495284	22.73845649142951	26.137948002952516
26	29.48207988189945	22.16025588452391	21.438530304272945	26.9191339293037
27	27.126219962273435	21.64561633724268	24.667842204543593	26.560321495940293
28	26.62388255556467	23.078815713934226	20.98745181661609	29.309849913885017
29	27.67366521774789	23.599606331501683	21.31345854178627	27.41326990896416
30	26.923234642827854	21.534897072090544	24.22701550069712	27.31485278438448
31	28.955138194045766	22.01878126794062	21.901910932502254	27.124169605511362
32	26.45370294431231	21.389321741983107	21.88140736488149	30.275567948823095
33	26.279422619535797	21.208890346920363	24.641187566636592	27.87049946690724
34	26.15230050028705	21.61691134257361	23.65496596407775	28.575822193061594
35	26.131796932666283	23.69187238579513	24.284425490035268	25.891905191503323
36	26.67514147461658	21.81374559173296	24.712950053309278	26.79816288034118
37	27.482982038874766	21.71122775362913	23.808742721233493	26.99704748626261
38	26.15025014352497	20.923890756991717	25.00205035676208	27.923808742721235
39	25.72992700729927	19.632165996883458	26.244566554580494	28.393340441236774
40	25.35676207660133	20.69425079963914	27.759780201755106	26.189206922004427
41	23.378167801197407	20.38464692856557	27.895103748052165	28.34208152218486
42	21.07151644386123	20.649142950873454	30.564668252275894	27.71467235298942
43	20.271877306651355	22.184860165668827	28.651685393258425	28.891577134421386
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	3.5
7	5.0
8	4.5
9	4.0
10	5.0
11	6.0
12	6.0
13	18.5
14	31.0
15	51.5
16	72.0
17	64.0
18	56.0
19	56.0
20	70.0
21	84.0
22	64.5
23	45.0
24	49.0
25	53.0
26	53.0
27	64.5
28	76.0
29	90.0
30	104.0
31	125.5
32	147.0
33	147.0
34	209.0
35	271.0
36	274.0
37	277.0
38	415.5
39	554.0
40	554.0
41	782.5
42	1011.0
43	1324.0
44	1637.0
45	2691.5
46	3746.0
47	3746.0
48	4300.5
49	4855.0
50	5458.5
51	6062.0
52	6189.5
53	6317.0
54	6317.0
55	5815.0
56	5313.0
57	5188.0
58	5063.0
59	4712.5
60	4362.0
61	4362.0
62	3789.0
63	3216.0
64	2603.5
65	1991.0
66	1647.0
67	1303.0
68	1303.0
69	1099.5
70	896.0
71	739.5
72	583.0
73	419.5
74	256.0
75	256.0
76	212.5
77	169.0
78	134.5
79	100.0
80	74.0
81	48.0
82	48.0
83	39.0
84	30.0
85	22.0
86	14.0
87	11.5
88	9.0
89	9.0
90	5.0
91	1.0
92	1.5
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	48772.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.93758714016239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.44715960680588	43.3404412367752
2	7.137499506533497	7.414090051668991
3	2.8542102562078084	4.447223816944149
4	1.4172357980340295	2.9443123103419997
5	0.9553511507638862	2.4809316821126877
6	0.6118984643322412	1.9068317887312392
7	0.5211006276893925	1.8945296481587799
8	0.33555722237574515	1.394242598212089
9	0.3158185622359954	1.476256868695153
>10	2.1475662232047688	21.504141720659394
>50	0.19343886936954718	6.8358894447633896
>100	0.0631637124471991	4.361108832936931
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	244	0.5002870499466907	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	176	0.36086279012548184	RNA PCR Primer, Index 47 (95% over 22bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	165	0.33830886574263924	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	164	0.3362585089805626	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	132	0.27064709259411135	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	132	0.27064709259411135	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	125	0.25629459525957515	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	121	0.24809316821126876	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	113	0.23169031411465596	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	112	0.22963995735257933	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	112	0.22963995735257933	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	112	0.22963995735257933	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	111	0.22758960059050276	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	105	0.21528746001804314	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	102	0.2091363897318133	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	101	0.20708603296973674	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	100	0.2050356762076601	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	99	0.20298531944558354	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	98	0.2009349626835069	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	98	0.2009349626835069	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	97	0.19888460592143034	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	94	0.19273353563520051	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	94	0.19273353563520051	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	86	0.17633068153858772	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	84	0.17222996801443452	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	83	0.17017961125235792	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	83	0.17017961125235792	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	80	0.1640285409661281	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	80	0.1640285409661281	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	77	0.1578774706798983	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	72	0.1476256868695153	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	71	0.1455753301074387	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	71	0.1455753301074387	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	70	0.1435249733453621	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	69	0.1414746165832855	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	66	0.13532354629705567	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	66	0.13532354629705567	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	65	0.13327318953497908	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	65	0.13327318953497908	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	64	0.13122283277290248	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	64	0.13122283277290248	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	64	0.13122283277290248	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	63	0.12917247601082588	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	60	0.12302140572459609	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	60	0.12302140572459609	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	59	0.12097104896251946	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	58	0.11892069220044287	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	58	0.11892069220044287	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	57	0.11687033543836627	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	57	0.11687033543836627	No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC	57	0.11687033543836627	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	56	0.11481997867628967	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	55	0.11276962191421308	No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT	55	0.11276962191421308	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	54	0.11071926515213648	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	54	0.11071926515213648	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	54	0.11071926515213648	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	54	0.11071926515213648	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	53	0.10866890839005988	No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG	52	0.10661855162798328	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	52	0.10661855162798328	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	52	0.10661855162798328	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	52	0.10661855162798328	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	51	0.10456819486590666	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	51	0.10456819486590666	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	50	0.10251783810383006	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	50	0.10251783810383006	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	50	0.10251783810383006	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	49	0.10046748134175346	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	49	0.10046748134175346	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.006151070286229804	0.0
25	0.0	0.0	0.0	0.008201427048306405	0.0
26	0.0	0.0	0.0	0.012302140572459609	0.0
27	0.0	0.0	0.0	0.03075535143114902	0.0
28	0.0	0.0	0.0	0.11276962191421307	0.0
29	0.0	0.0	0.0	0.25219388173542195	0.0
30	0.0	0.0	0.0	0.4592799147051587	0.0
31	0.0	0.0	0.0	0.9267612564586238	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	25	0.0054455064	29.6	37
ACGGTAC	25	0.0054455064	29.6	3
GCGGTAA	90	5.456968E-12	26.722221	23
CGCGGTA	90	5.456968E-12	26.722221	22
ACGCTTG	35	8.74765E-4	26.42857	25
AACTCCG	95	9.094947E-12	25.315786	5
TAATACG	95	9.094947E-12	25.315786	27
TCCGTGC	95	9.094947E-12	25.315786	8
CTCCGTG	95	9.094947E-12	25.315786	7
CGGTAAT	95	9.094947E-12	25.315786	24
GGTAATA	95	9.094947E-12	25.315786	25
GGCTAAC	90	1.3278623E-10	24.666666	1
TAACTCC	100	1.8189894E-11	24.050001	4
CTAACTC	100	1.8189894E-11	24.050001	3
GCTAACT	100	1.8189894E-11	24.050001	2
CCGCGGT	100	1.8189894E-11	24.050001	21
GGTATCA	110	1.8189894E-12	23.545454	1
AACGCTT	40	0.0019049287	23.125002	24
AGCGTAC	40	0.0019049287	23.125002	1
TCCGATT	40	0.0019049287	23.125002	17
>>END_MODULE