ERR1632618.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1632618.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37380
Sequences flagged as poor quality	0
Sequence length	43
%GC	54

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	188	0.5029427501337613	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	181	0.4842161583734617	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	107	0.28624933119315144	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	103	0.27554842161583737	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	103	0.27554842161583737	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	90	0.2407704654895666	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	89	0.2380952380952381	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	85	0.22739432851792402	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	84	0.22471910112359553	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	81	0.21669341894060995	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	79	0.21134296415195294	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	76	0.20331728196896737	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	75	0.20064205457463882	TruSeq Adapter, Index 1 (95% over 21bp)
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	74	0.19796682718031033	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	73	0.19529159978598182	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	72	0.1926163723916533	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	72	0.1926163723916533	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	71	0.18994114499732476	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	69	0.18459069020866772	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	65	0.17388978063135366	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	64	0.17121455323702514	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	63	0.16853932584269662	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	62	0.1658640984483681	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	61	0.1631888710540396	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	61	0.1631888710540396	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	61	0.1631888710540396	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	60	0.16051364365971107	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	59	0.15783841626538256	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	59	0.15783841626538256	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	59	0.15783841626538256	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	58	0.15516318887105404	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	58	0.15516318887105404	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	57	0.15248796147672553	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	56	0.149812734082397	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	55	0.1471375066880685	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	55	0.1471375066880685	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	55	0.1471375066880685	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	54	0.14446227929373998	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	54	0.14446227929373998	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	54	0.14446227929373998	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	53	0.14178705189941146	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	53	0.14178705189941146	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	52	0.13911182450508291	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	51	0.13643659711075443	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	51	0.13643659711075443	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	51	0.13643659711075443	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	51	0.13643659711075443	No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	51	0.13643659711075443	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	50	0.13376136971642588	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	49	0.1310861423220974	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	48	0.12841091492776885	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	48	0.12841091492776885	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	48	0.12841091492776885	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	47	0.12573568753344033	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	47	0.12573568753344033	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	47	0.12573568753344033	No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA	45	0.1203852327447833	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	45	0.1203852327447833	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	45	0.1203852327447833	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	43	0.11503477795612627	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	43	0.11503477795612627	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	43	0.11503477795612627	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	42	0.11235955056179776	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	42	0.11235955056179776	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	41	0.10968432316746923	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	41	0.10968432316746923	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	40	0.1070090957731407	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40	0.1070090957731407	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	39	0.1043338683788122	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	38	0.10165864098448368	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	38	0.10165864098448368	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	35	2.3274746E-5	31.714285	37
TGGTTCA	30	3.5338086E-4	30.833334	5
GTACTGG	30	3.5338086E-4	30.833334	1
CTGGTTC	30	3.5338086E-4	30.833334	4
TACTGGT	30	3.5338086E-4	30.833334	2
ACTGGTT	30	3.5338086E-4	30.833334	3
GGTCTGT	25	0.005429368	29.6	36
GGTATCA	105	0.0	28.190475	1
AACTCCG	95	6.6047505E-9	21.421053	5
TAACTCC	90	8.878487E-8	20.555555	4
CTAACTC	90	8.878487E-8	20.555555	3
ATGCACA	45	0.0037579644	20.555555	29
TCCGTGC	90	8.878487E-8	20.555555	8
GTATCAA	145	0.0	20.413794	2
GCTAACT	95	1.57057E-7	19.473684	2
CTCCGTG	95	1.57057E-7	19.473684	7
ACTCCGT	115	2.7812348E-9	19.304348	6
TTCACTA	50	0.006911317	18.5	8
TGCACAT	50	0.006911317	18.5	30
CCGTGCC	100	2.6947964E-7	18.5	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content