##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632618.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37380
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31904761904762	31.0	31.0	34.0	30.0	34.0
2	31.51530230069556	31.0	31.0	34.0	30.0	34.0
3	31.621107544141253	31.0	31.0	34.0	30.0	34.0
4	35.413456393793474	37.0	35.0	37.0	33.0	37.0
5	34.54545211342964	35.0	35.0	37.0	30.0	37.0
6	34.35751738897806	35.0	35.0	37.0	30.0	37.0
7	34.387907972177636	35.0	35.0	37.0	30.0	37.0
8	34.27739432851792	35.0	35.0	37.0	30.0	37.0
9	35.632878544676295	37.0	35.0	39.0	30.0	39.0
10	35.525468164794006	37.0	35.0	39.0	30.0	39.0
11	35.56720171214553	37.0	35.0	39.0	30.0	39.0
12	35.46147672552167	37.0	35.0	39.0	30.0	39.0
13	35.6229266987694	37.0	35.0	39.0	30.0	39.0
14	36.45168539325843	38.0	35.0	40.0	31.0	41.0
15	36.45115034777956	38.0	35.0	40.0	31.0	41.0
16	36.34697699304441	38.0	35.0	40.0	30.0	41.0
17	36.304200107009095	38.0	35.0	40.0	30.0	41.0
18	36.32734082397004	38.0	35.0	40.0	30.0	41.0
19	36.274264312466556	38.0	35.0	40.0	30.0	41.0
20	36.27972177635099	38.0	35.0	40.0	30.0	41.0
21	36.17043873729267	38.0	34.0	40.0	30.0	41.0
22	36.06417870518994	38.0	34.0	40.0	29.0	41.0
23	36.0123595505618	38.0	34.0	40.0	29.0	41.0
24	35.942081326912785	38.0	34.0	40.0	29.0	41.0
25	35.838095238095235	38.0	34.0	40.0	29.0	41.0
26	35.596896736222575	38.0	34.0	40.0	28.0	41.0
27	35.415783841626535	38.0	34.0	40.0	27.0	41.0
28	35.31294810058855	38.0	33.0	40.0	27.0	41.0
29	35.29491706795078	38.0	33.0	40.0	27.0	41.0
30	35.19250936329588	38.0	33.0	40.0	27.0	41.0
31	35.0619315141787	38.0	33.0	40.0	27.0	41.0
32	34.95690208667737	37.0	33.0	40.0	26.0	41.0
33	34.85904226859283	37.0	33.0	40.0	26.0	41.0
34	34.817308721241304	37.0	33.0	40.0	26.0	41.0
35	34.64470304975923	37.0	33.0	39.0	26.0	40.0
36	34.52907972177635	37.0	33.0	39.0	26.0	40.0
37	34.45468164794008	37.0	32.0	39.0	26.0	40.0
38	34.30096308186196	37.0	32.0	39.0	25.0	40.0
39	34.139111824505086	37.0	32.0	39.0	24.0	40.0
40	34.03199571963617	36.0	32.0	39.0	24.0	40.0
41	33.93322632423756	36.0	32.0	39.0	24.0	40.0
42	33.80307651150348	36.0	32.0	39.0	24.0	40.0
43	32.84328517924023	35.0	30.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	3.0
17	2.0
18	8.0
19	19.0
20	26.0
21	35.0
22	78.0
23	117.0
24	172.0
25	266.0
26	377.0
27	552.0
28	809.0
29	1036.0
30	1398.0
31	1857.0
32	2364.0
33	2972.0
34	3654.0
35	4330.0
36	4984.0
37	5109.0
38	4850.0
39	2360.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.266452648475116	16.056714820759765	13.113964686998395	27.56286784376672
2	22.790262172284645	20.679507758159442	30.529695024077046	26.000535045478866
3	23.21027287319422	19.07437132156233	28.83092562867844	28.884430176565008
4	16.39111824505083	16.813804173354736	34.88228999464955	31.91278758694489
5	18.237025147137505	31.562332798287855	31.875334403424294	18.325307651150347
6	30.834670947030496	34.73515248796148	16.615837346174427	17.8143392188336
7	28.924558587479936	27.220438737292667	19.119850187265918	24.735152487961475
8	26.342964151952913	29.32851792402354	21.128945960406632	23.199571963616908
9	27.833065810593897	12.707330123060459	16.837881219903693	42.62172284644195
10	20.628678437667205	21.51417870518994	27.74745853397539	30.109684323167468
11	39.76993044408775	19.63884430176565	18.41091492776886	22.180310326377743
12	21.452648475120384	23.290529695024077	26.94756554307116	28.30925628678438
13	37.504012841091495	17.011771000535045	19.62011771000535	25.86409844836811
14	24.2215088282504	20.23542001070091	22.953451043338685	32.589620117710005
15	31.227929373996787	22.827715355805246	19.36597110754414	26.578384162653823
16	24.363295880149813	23.336008560727663	23.584804708400213	28.71589085072231
17	27.23381487426431	23.202247191011235	20.95773140716961	28.60620652755484
18	26.76565008025682	20.377207062600323	22.819689673622257	30.0374531835206
19	28.472445157838415	22.48796147672552	22.913322632423753	26.12627073301231
20	31.37239165329053	19.826110219368644	21.792402354200107	27.009095773140718
21	28.58747993579454	20.893525949705722	21.476725521669344	29.04226859283039
22	28.713215623327983	22.338148742643124	20.754414125200643	28.19422150882825
23	28.924558587479936	20.567148207597647	22.011771000535045	28.496522204387375
24	28.55270197966827	20.68218298555377	22.450508293204923	28.314606741573034
25	29.371321562332795	21.511503477795614	22.1856607811664	26.93151417870519
26	28.983413590155166	22.426431246655966	21.62386302835741	26.96629213483146
27	27.522739432851793	21.350989834135902	24.9063670411985	26.219903691813805
28	27.340823970037455	23.092562867843768	20.81326912787587	28.75334403424291
29	28.025682182985555	23.675762439807382	20.95505617977528	27.343499197431782
30	27.316746923488495	21.511503477795614	24.028892455858745	27.142857142857142
31	29.307116104868914	21.3697164258962	22.02247191011236	27.300695559122524
32	26.13429641519529	21.436597110754413	22.926698769395397	29.5024077046549
33	26.49545211342964	20.965757089352596	23.836276083467094	28.70251471375067
34	25.92562867843767	22.27394328517924	23.47244515783842	28.327982878544677
35	26.567683253076513	23.600856072766184	23.164794007490634	26.666666666666668
36	27.63777421080792	20.995184590690208	24.57196361690744	26.795077581594434
37	28.75601926163724	20.411985018726593	23.60888175494917	27.223113964686995
38	26.59711075441413	20.44676297485286	24.74852862493312	28.207597645799893
39	26.01391118245051	19.74585339753879	26.30016051364366	27.94007490636704
40	26.12359550561798	20.789192081326913	26.93151417870519	26.155698234349924
41	24.026217228464418	20.168539325842698	27.86516853932584	27.94007490636704
42	21.120920278223647	21.273408239700377	29.523809523809526	28.08186195826645
43	21.20652755484216	22.372926698769398	27.833065810593897	28.58747993579454
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.5
4	2.0
5	2.0
6	2.0
7	2.0
8	3.0
9	4.0
10	5.5
11	7.0
12	7.0
13	13.5
14	20.0
15	36.0
16	52.0
17	58.0
18	64.0
19	64.0
20	71.5
21	79.0
22	51.5
23	24.0
24	24.5
25	25.0
26	25.0
27	21.0
28	17.0
29	16.5
30	16.0
31	22.5
32	29.0
33	29.0
34	68.0
35	107.0
36	97.0
37	87.0
38	194.0
39	301.0
40	301.0
41	515.5
42	730.0
43	949.0
44	1168.0
45	1992.5
46	2817.0
47	2817.0
48	3328.5
49	3840.0
50	4370.5
51	4901.0
52	4998.0
53	5095.0
54	5095.0
55	4760.5
56	4426.0
57	4109.5
58	3793.0
59	3497.5
60	3202.0
61	3202.0
62	2847.0
63	2492.0
64	1946.0
65	1400.0
66	1205.0
67	1010.0
68	1010.0
69	852.0
70	694.0
71	573.5
72	453.0
73	342.5
74	232.0
75	232.0
76	188.5
77	145.0
78	114.0
79	83.0
80	59.0
81	35.0
82	35.0
83	25.5
84	16.0
85	12.0
86	8.0
87	5.5
88	3.0
89	3.0
90	1.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	37380.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.43392188336009
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.27920524290025	42.31942215088282
2	7.261000728180589	7.469234884965221
3	2.954332674503277	4.558587479935794
4	1.7892437324456465	3.6811128945960405
5	1.1234786226984292	2.889245585874799
6	0.728180588786019	2.247191011235955
7	0.6033496307084157	2.1722846441947565
8	0.37449287423280975	1.5409309791332264
9	0.3536877145532092	1.637239165329053
>10	2.283366274836159	22.34884965222044
>50	0.2236554665557058	7.311396468699839
>100	0.026006449599500672	1.8245050829320493
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	188	0.5029427501337613	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	181	0.4842161583734617	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	107	0.28624933119315144	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	103	0.27554842161583737	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	103	0.27554842161583737	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	90	0.2407704654895666	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	89	0.2380952380952381	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	85	0.22739432851792402	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	84	0.22471910112359553	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	81	0.21669341894060995	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	79	0.21134296415195294	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	76	0.20331728196896737	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	75	0.20064205457463882	TruSeq Adapter, Index 1 (95% over 21bp)
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	74	0.19796682718031033	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	73	0.19529159978598182	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	72	0.1926163723916533	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	72	0.1926163723916533	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	71	0.18994114499732476	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	69	0.18459069020866772	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	65	0.17388978063135366	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	64	0.17121455323702514	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	63	0.16853932584269662	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	62	0.1658640984483681	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	61	0.1631888710540396	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	61	0.1631888710540396	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	61	0.1631888710540396	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	60	0.16051364365971107	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	59	0.15783841626538256	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	59	0.15783841626538256	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	59	0.15783841626538256	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	58	0.15516318887105404	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	58	0.15516318887105404	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	57	0.15248796147672553	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	56	0.149812734082397	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	55	0.1471375066880685	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	55	0.1471375066880685	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	55	0.1471375066880685	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	54	0.14446227929373998	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	54	0.14446227929373998	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	54	0.14446227929373998	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	53	0.14178705189941146	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	53	0.14178705189941146	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	52	0.13911182450508291	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	51	0.13643659711075443	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	51	0.13643659711075443	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	51	0.13643659711075443	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	51	0.13643659711075443	No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	51	0.13643659711075443	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	50	0.13376136971642588	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	49	0.1310861423220974	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	48	0.12841091492776885	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	48	0.12841091492776885	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	48	0.12841091492776885	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	47	0.12573568753344033	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	47	0.12573568753344033	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	47	0.12573568753344033	No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA	45	0.1203852327447833	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	45	0.1203852327447833	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	45	0.1203852327447833	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	43	0.11503477795612627	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	43	0.11503477795612627	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	43	0.11503477795612627	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	42	0.11235955056179776	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	42	0.11235955056179776	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	41	0.10968432316746923	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	41	0.10968432316746923	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	40	0.1070090957731407	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40	0.1070090957731407	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	39	0.1043338683788122	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	38	0.10165864098448368	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	38	0.10165864098448368	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.002675227394328518
15	0.0	0.0	0.0	0.0	0.002675227394328518
16	0.0	0.0	0.0	0.0	0.002675227394328518
17	0.0	0.0	0.0	0.0	0.002675227394328518
18	0.0	0.0	0.0	0.0	0.002675227394328518
19	0.0	0.0	0.0	0.0	0.002675227394328518
20	0.0	0.0	0.0	0.0	0.002675227394328518
21	0.0	0.0	0.0	0.005350454788657036	0.002675227394328518
22	0.0	0.0	0.0	0.005350454788657036	0.002675227394328518
23	0.0	0.0	0.0	0.005350454788657036	0.002675227394328518
24	0.0	0.0	0.0	0.008025682182985553	0.002675227394328518
25	0.0	0.0	0.0	0.008025682182985553	0.002675227394328518
26	0.0	0.0	0.0	0.008025682182985553	0.002675227394328518
27	0.0	0.0	0.0	0.03210272873194221	0.002675227394328518
28	0.0	0.0	0.0	0.13911182450508294	0.002675227394328518
29	0.0	0.0	0.0	0.30497592295345105	0.002675227394328518
30	0.0	0.0	0.0	0.48421615837346177	0.002675227394328518
31	0.0	0.0	0.0	1.0085607276618513	0.002675227394328518
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	35	2.3274746E-5	31.714285	37
TGGTTCA	30	3.5338086E-4	30.833334	5
GTACTGG	30	3.5338086E-4	30.833334	1
CTGGTTC	30	3.5338086E-4	30.833334	4
TACTGGT	30	3.5338086E-4	30.833334	2
ACTGGTT	30	3.5338086E-4	30.833334	3
GGTCTGT	25	0.005429368	29.6	36
GGTATCA	105	0.0	28.190475	1
AACTCCG	95	6.6047505E-9	21.421053	5
TAACTCC	90	8.878487E-8	20.555555	4
CTAACTC	90	8.878487E-8	20.555555	3
ATGCACA	45	0.0037579644	20.555555	29
TCCGTGC	90	8.878487E-8	20.555555	8
GTATCAA	145	0.0	20.413794	2
GCTAACT	95	1.57057E-7	19.473684	2
CTCCGTG	95	1.57057E-7	19.473684	7
ACTCCGT	115	2.7812348E-9	19.304348	6
TTCACTA	50	0.006911317	18.5	8
TGCACAT	50	0.006911317	18.5	30
CCGTGCC	100	2.6947964E-7	18.5	9
>>END_MODULE