FastQCFastQC Report
Fri 10 Feb 2017
ERR1632617.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632617.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468046
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7550.16130893117343167No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6390.13652504241036137No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4820.10298133089482657No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4750.10148575140050337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4550.029.2747251
GGGTACT701.9178515E-723.7857131
GTCGACA400.00192948623.12522
TGAAGCG400.00192948623.1255
GTCCTAG400.00192948623.1251
ATACGGC652.6772068E-622.7692329
TTGTACT502.6987866E-422.24
TTATCCT603.720213E-521.5833344
GCCGTCT705.091266E-621.14285736
GCGGTAA3350.020.98507523
GGTACTT806.941991E-720.81252
GTATCAA6450.020.6511632
TAATACG3050.020.62295227
ACCTAGC450.00382247120.5555551
TATACAC1500.019.73333437
CGGTAAT3600.019.52777724
CTTATAC4750.019.0842137
GTAATAC3500.019.02857226
TCTTATA9050.018.6022137
TCGTTTA500.007028803718.529
Produced by FastQC (version 0.11.5)