##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632617.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468046
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66738098392038	34.0	31.0	34.0	31.0	34.0
2	32.83403981659922	34.0	31.0	34.0	31.0	34.0
3	32.9270755438568	34.0	31.0	34.0	31.0	34.0
4	36.36372706956154	37.0	37.0	37.0	35.0	37.0
5	36.23722454630528	37.0	37.0	37.0	35.0	37.0
6	36.25649188327643	37.0	37.0	37.0	35.0	37.0
7	36.25284480585242	37.0	37.0	37.0	35.0	37.0
8	36.21892933600544	37.0	37.0	37.0	35.0	37.0
9	37.94462723749375	39.0	38.0	39.0	35.0	39.0
10	37.92032834379527	39.0	38.0	39.0	35.0	39.0
11	38.013265790114644	39.0	38.0	39.0	35.0	39.0
12	37.95212650038671	39.0	38.0	39.0	35.0	39.0
13	38.005514415249785	39.0	38.0	39.0	35.0	39.0
14	39.414412258624154	40.0	39.0	41.0	37.0	41.0
15	39.3829153544737	40.0	39.0	41.0	37.0	41.0
16	39.3587318340505	40.0	39.0	41.0	37.0	41.0
17	39.34466270409319	40.0	39.0	41.0	37.0	41.0
18	39.331027292189226	40.0	39.0	41.0	37.0	41.0
19	39.341265602098936	40.0	39.0	41.0	37.0	41.0
20	39.30921319699346	40.0	39.0	41.0	37.0	41.0
21	39.274981518910536	40.0	39.0	41.0	36.0	41.0
22	39.22822329429159	40.0	39.0	41.0	36.0	41.0
23	39.17359404844823	40.0	39.0	41.0	36.0	41.0
24	39.15901855800498	40.0	39.0	41.0	36.0	41.0
25	39.0905594749234	40.0	39.0	41.0	36.0	41.0
26	39.006815569409845	40.0	39.0	41.0	36.0	41.0
27	38.91739914452853	40.0	38.0	41.0	35.0	41.0
28	38.85807164253086	40.0	38.0	41.0	35.0	41.0
29	38.806666438768836	40.0	38.0	41.0	35.0	41.0
30	38.75131504168394	40.0	38.0	41.0	35.0	41.0
31	38.68744311456566	40.0	38.0	41.0	35.0	41.0
32	38.62846814202023	40.0	38.0	41.0	35.0	41.0
33	38.558274186725235	40.0	38.0	41.0	35.0	41.0
34	38.506847617541865	40.0	38.0	41.0	35.0	41.0
35	38.42813099567137	40.0	38.0	41.0	34.0	41.0
36	38.37482854249369	40.0	38.0	41.0	34.0	41.0
37	38.32125047538062	40.0	38.0	41.0	34.0	41.0
38	38.251789354037854	40.0	38.0	41.0	34.0	41.0
39	38.17008370972084	40.0	38.0	41.0	34.0	41.0
40	38.08128474551646	40.0	37.0	41.0	34.0	41.0
41	38.03247330390602	40.0	37.0	41.0	34.0	41.0
42	37.96040132807459	40.0	37.0	41.0	34.0	41.0
43	37.15731146083932	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	5.0
17	8.0
18	18.0
19	25.0
20	49.0
21	80.0
22	160.0
23	272.0
24	436.0
25	580.0
26	813.0
27	1263.0
28	1610.0
29	2338.0
30	3119.0
31	4022.0
32	5611.0
33	7430.0
34	11412.0
35	17632.0
36	29368.0
37	57156.0
38	140660.0
39	183978.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.55597526738825	16.668447118445624	12.086846164693215	27.688731449472915
2	21.716668874426873	19.105387077338552	31.55309520859061	27.624848839643967
3	21.436568200561485	19.883302068600095	28.190818851138566	30.48931087969986
4	16.428086128286537	14.863496323010986	32.878178640560975	35.83023890814151
5	17.652538425710294	32.54658730124817	32.96599052229909	16.83488375074245
6	36.96089700584985	32.88010152848224	13.977899608158173	16.181101857509734
7	31.604372219824544	27.070629809890477	19.299171448960145	22.025826521324827
8	28.556167556180373	30.655747511996683	19.07462087059819	21.71346406122475
9	28.37520243736727	12.52312806860864	17.05281104848669	42.048858445537405
10	19.495519671143434	23.334672233071107	29.80433547129983	27.365472624485626
11	40.51717138913696	18.86011204026955	18.269358140011878	22.35335843058161
12	24.092931036692974	23.239382453861374	25.869038513308524	26.79864799613713
13	35.17923451968396	17.285053178533737	20.142678283758432	27.393034018023872
14	24.871059682167992	18.688334052635852	22.22174743508117	34.21885883011499
15	29.443259850527514	23.624173692329386	19.754468577874825	27.178097879268275
16	27.37679629779979	23.157552890100547	21.255175773321426	28.210475038778238
17	27.497297274199546	22.742636407532594	21.482931164885503	28.27713515338236
18	27.10267794191169	21.424817218820372	22.994321071005842	28.478183768262095
19	28.85977019352799	21.645094712912833	22.403139862321225	27.091995231237952
20	29.841724958657913	20.79966499019327	22.196749892104624	27.1618601590442
21	28.12629527866919	21.923058844643474	21.480153660110332	28.470492216577004
22	28.92856685026685	22.402712553894276	20.783640924182667	27.885079671656204
23	28.563218145225044	21.951688509249088	21.648726834541904	27.83636651098396
24	28.642270204210696	21.50985159578332	21.35666152472193	28.491216675284054
25	28.795246621058613	21.57950286937609	21.97818163171996	27.64706887784534
26	28.559372369382498	21.936305405878908	21.532071633984696	27.9722505907539
27	27.863073287668307	21.729060818808406	22.516376595462837	27.891489298060446
28	27.034522247813246	22.321310298560398	21.845288710938668	28.798878742687684
29	27.781671032334426	22.786649175508387	21.879687039308102	27.55199275284908
30	27.602842455656067	21.860458160095376	22.719775406690797	27.81692397755776
31	27.954089982608547	22.0683437098063	21.910453246048466	28.067113061536688
32	26.13055127060161	21.931605013182466	22.344171299402195	29.59367241681373
33	26.711263422825958	21.6309935348235	23.556017998230942	28.101725044119597
34	26.937950543322664	21.135743067989043	23.701516517607242	28.224789871081047
35	26.599094960751724	22.38412463732197	23.75407545412203	27.262704947804277
36	26.753353302880484	21.434858966853685	24.211936433598407	27.59985129666742
37	26.534571388282345	21.04194886827363	24.275391735000408	28.148088008443615
38	25.724180956572646	20.364237703131742	25.587655914162283	28.32392542613333
39	24.898621075706235	19.955517192754556	26.70699033855647	28.43887139298274
40	24.87682834593181	20.419360490208227	27.578913183746895	27.124897980113065
41	23.783132427154598	19.64614589164313	28.29486845310076	28.27585322810151
42	22.308704699965386	20.460382099195378	29.443046196314036	27.787867004525197
43	21.74935796908851	20.310396841336107	29.26037184379313	28.67987334578225
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	4.0
4	8.0
5	8.0
6	15.0
7	22.0
8	22.0
9	22.0
10	28.5
11	35.0
12	35.0
13	69.0
14	103.0
15	172.0
16	241.0
17	271.0
18	301.0
19	301.0
20	358.5
21	416.0
22	451.0
23	486.0
24	637.0
25	788.0
26	788.0
27	1043.5
28	1299.0
29	1693.0
30	2087.0
31	2767.5
32	3448.0
33	3448.0
34	4657.0
35	5866.0
36	6810.0
37	7754.0
38	9815.0
39	11876.0
40	11876.0
41	13767.5
42	15659.0
43	18277.5
44	20896.0
45	24537.0
46	28178.0
47	28178.0
48	32037.0
49	35896.0
50	39370.0
51	42844.0
52	45202.5
53	47561.0
54	47561.0
55	46754.5
56	45948.0
57	45018.5
58	44089.0
59	42284.5
60	40480.0
61	40480.0
62	37077.5
63	33675.0
64	28787.5
65	23900.0
66	20927.5
67	17955.0
68	17955.0
69	15244.0
70	12533.0
71	10560.0
72	8587.0
73	7000.5
74	5414.0
75	5414.0
76	4402.0
77	3390.0
78	2828.5
79	2267.0
80	1952.5
81	1638.0
82	1638.0
83	1414.0
84	1190.0
85	853.5
86	517.0
87	449.0
88	381.0
89	381.0
90	275.5
91	170.0
92	125.5
93	81.0
94	59.5
95	38.0
96	38.0
97	20.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	468046.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.42762092749889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.8336469690882	56.34506885562589
2	5.814871928744792	7.376469848767582
3	1.749046275419052	3.328135324258004
4	0.8695013673899259	2.206016125070007
5	0.5416229981606228	1.7176929106473708
6	0.3898974216757135	1.4838159517593807
7	0.2596593771564284	1.1528703581183886
8	0.19531569367412316	0.9910727823643023
9	0.14204345366845564	0.8108530501064001
>10	1.0323976745893	12.497701149509739
>50	0.10677996387804506	4.674517331943873
>100	0.06454105400037437	7.1170115685143545
>500	6.75822554977742E-4	0.2987747433147326
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	755	0.16130893117343167	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	639	0.13652504241036137	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	482	0.10298133089482657	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	475	0.10148575140050337	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.1365421347474393E-4	0.0	0.0	0.0	0.0
9	2.1365421347474393E-4	0.0	0.0	0.0	0.0
10	2.1365421347474393E-4	0.0	0.0	0.0	0.0
11	2.1365421347474393E-4	0.0	0.0	0.0	0.0
12	2.1365421347474393E-4	0.0	0.0	0.0	0.0
13	2.1365421347474393E-4	0.0	0.0	0.0	0.0
14	2.1365421347474393E-4	0.0	0.0	0.0	0.0
15	2.1365421347474393E-4	0.0	0.0	0.0	0.0
16	2.1365421347474393E-4	0.0	0.0	0.0	0.0
17	2.1365421347474393E-4	0.0	0.0	8.546168538989757E-4	0.0
18	4.2730842694948786E-4	0.0	0.0	0.0010682710673737196	0.0
19	4.2730842694948786E-4	0.0	0.0	0.0010682710673737196	0.0
20	4.2730842694948786E-4	0.0	0.0	0.0014955794943232075	0.0
21	6.409626404242318E-4	0.0	0.0	0.0021365421347474393	0.0
22	6.409626404242318E-4	0.0	0.0	0.003632121629070647	0.0
23	6.409626404242318E-4	0.0	0.0	0.005127701123393854	0.0
24	6.409626404242318E-4	0.0	0.0	0.0070505890446665494	0.0
25	6.409626404242318E-4	0.0	0.0	0.008546168538989757	0.0
26	6.409626404242318E-4	0.0	0.0	0.011323673314161429	0.0
27	6.409626404242318E-4	0.0	0.0	0.03140716938078736	0.0
28	6.409626404242318E-4	0.0	0.0	0.13353388342171496	0.0
29	6.409626404242318E-4	0.0	0.0	0.2858693376292074	0.0
30	6.409626404242318E-4	0.0	0.0	0.4958914294748807	0.0
31	6.409626404242318E-4	0.0	0.0	1.0699803010815176	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	455	0.0	29.274725	1
GGGTACT	70	1.9178515E-7	23.785713	1
GTCGACA	40	0.001929486	23.125	22
TGAAGCG	40	0.001929486	23.125	5
GTCCTAG	40	0.001929486	23.125	1
ATACGGC	65	2.6772068E-6	22.76923	29
TTGTACT	50	2.6987866E-4	22.2	4
TTATCCT	60	3.720213E-5	21.583334	4
GCCGTCT	70	5.091266E-6	21.142857	36
GCGGTAA	335	0.0	20.985075	23
GGTACTT	80	6.941991E-7	20.8125	2
GTATCAA	645	0.0	20.651163	2
TAATACG	305	0.0	20.622952	27
ACCTAGC	45	0.003822471	20.555555	1
TATACAC	150	0.0	19.733334	37
CGGTAAT	360	0.0	19.527777	24
CTTATAC	475	0.0	19.08421	37
GTAATAC	350	0.0	19.028572	26
TCTTATA	905	0.0	18.60221	37
TCGTTTA	50	0.0070288037	18.5	29
>>END_MODULE