FastQCFastQC Report
Fri 10 Feb 2017
ERR1632615.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632615.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236551
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5100.21559832763336445No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3860.16317834209113466No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3380.14288673478446506No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3180.1344318984066861No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2850.12048141838335075No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2690.11371754928112754No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2690.11371754928112754No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2600.10991287291112699No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2520.10653093836001537No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2510.10610819654112644No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2440.1031490038089038No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2420.10230352017112589No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2370.10018981107668112No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACTG250.00548763329.59999834
GTGTAAA250.00548763329.5999981
ACTAACT250.00548763329.59999833
GGTATCA2400.029.2916661
ACTACTA358.8485E-426.42857232
ATACGGC451.3188765E-424.66666829
GTAACGT1450.024.2413826
ATACACA400.001926680623.12500237
TGCGGGT1500.022.221
CGTCAAT1600.021.96875230
TGAGCAA1450.021.68965736
TCAATGA1550.021.48387132
GTATCAA3350.020.9850752
CGGGTAA1600.020.812523
TAAACCA450.003816965320.5555575
TTATACA1351.8189894E-1220.55555337
AACGTCA1650.020.18181828
GGTAACG1650.020.18181825
TATACAC555.1265146E-420.18181837
TAACGTC1750.020.08571427
Produced by FastQC (version 0.11.5)