##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632615.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	236551
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06904642127913	34.0	33.0	34.0	31.0	34.0
2	33.21285896064696	34.0	33.0	34.0	31.0	34.0
3	33.286462538733716	34.0	34.0	34.0	31.0	34.0
4	36.55926628929914	37.0	37.0	37.0	35.0	37.0
5	36.53021547150509	37.0	37.0	37.0	35.0	37.0
6	36.570337897535836	37.0	37.0	37.0	35.0	37.0
7	36.56276236414135	37.0	37.0	37.0	35.0	37.0
8	36.537841733917844	37.0	37.0	37.0	35.0	37.0
9	38.340383257733	39.0	39.0	39.0	37.0	39.0
10	38.36806439203385	39.0	39.0	39.0	37.0	39.0
11	38.42615757278557	39.0	39.0	39.0	37.0	39.0
12	38.39052043745323	39.0	39.0	39.0	37.0	39.0
13	38.42316033328965	39.0	39.0	39.0	37.0	39.0
14	39.98271831444382	41.0	40.0	41.0	38.0	41.0
15	39.97997894745742	41.0	40.0	41.0	38.0	41.0
16	39.95341385155844	41.0	40.0	41.0	38.0	41.0
17	39.942545159394804	41.0	40.0	41.0	38.0	41.0
18	39.939222408698335	41.0	40.0	41.0	38.0	41.0
19	39.943259593068724	41.0	40.0	41.0	38.0	41.0
20	39.91365498349193	41.0	40.0	41.0	38.0	41.0
21	39.874957197390835	41.0	40.0	41.0	38.0	41.0
22	39.8514569796788	41.0	40.0	41.0	38.0	41.0
23	39.81587902820111	41.0	40.0	41.0	38.0	41.0
24	39.797451712315734	41.0	40.0	41.0	38.0	41.0
25	39.7571348250483	41.0	40.0	41.0	38.0	41.0
26	39.675160958947544	41.0	40.0	41.0	37.0	41.0
27	39.58677409945424	41.0	40.0	41.0	37.0	41.0
28	39.54131667166911	41.0	40.0	41.0	37.0	41.0
29	39.49448956039078	41.0	40.0	41.0	37.0	41.0
30	39.44994102751627	41.0	39.0	41.0	37.0	41.0
31	39.39205921767399	41.0	39.0	41.0	36.0	41.0
32	39.346935755925784	41.0	39.0	41.0	36.0	41.0
33	39.264556057678895	41.0	39.0	41.0	36.0	41.0
34	39.23522200286619	41.0	39.0	41.0	35.0	41.0
35	39.15881987393839	41.0	39.0	41.0	35.0	41.0
36	39.11670633394067	41.0	39.0	41.0	35.0	41.0
37	39.066099910801476	41.0	39.0	41.0	35.0	41.0
38	38.99848658428838	41.0	39.0	41.0	35.0	41.0
39	38.89798394426572	40.0	39.0	41.0	35.0	41.0
40	38.823505290613866	40.0	38.0	41.0	35.0	41.0
41	38.76888704761341	40.0	38.0	41.0	35.0	41.0
42	38.7074415242379	40.0	38.0	41.0	35.0	41.0
43	37.979704165275145	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	0.0
18	6.0
19	3.0
20	11.0
21	32.0
22	49.0
23	75.0
24	137.0
25	156.0
26	273.0
27	370.0
28	512.0
29	758.0
30	1022.0
31	1302.0
32	1747.0
33	2387.0
34	3776.0
35	5807.0
36	9121.0
37	17395.0
38	44446.0
39	147164.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.950146057298426	16.44254304568571	12.384221584351788	28.22308931266408
2	21.725124814522026	19.072842642812756	31.35433796517453	27.84769457749069
3	21.02802355517415	19.42160464339614	28.610320818766354	30.940050982663358
4	16.085326208724545	15.222932898191088	32.95357026603143	35.73817062705294
5	17.724718982375894	32.494049908899136	32.61241761818805	17.168813490536923
6	36.032821674818535	32.72655790928806	14.663645471801006	16.576974944092395
7	31.021640153708923	27.015738677917234	19.648617000139502	22.314004168234334
8	28.051033392376272	30.432760799996615	19.57463718183394	21.94156862579317
9	28.185888032601845	12.53640863915181	17.30789554895139	41.969807779294946
10	19.305773385020565	23.175129253311123	29.531052500306487	27.98804486136182
11	40.39847643848473	18.861894475187167	18.472549259990444	22.26707982633766
12	23.740335065165652	23.25418197344336	26.13770392008489	26.867779041306108
13	35.31627429180177	17.175154617820258	20.3719282522585	27.136642838119474
14	24.604419342974666	19.19670599574722	22.221846451716544	33.977028209561574
15	29.325599976326462	23.937755494586792	19.374257559680576	27.362386969406177
16	26.53846316439161	23.55179221394118	21.807136727386485	28.102607894280723
17	27.758496053705123	22.89907884557664	21.541654865124222	27.800770235594012
18	27.313771660233943	21.190356413627505	22.708845027076613	28.787026899061935
19	28.83775591732861	21.839265105622044	22.479718961238802	26.843260015810543
20	29.89291949727543	20.96376679870303	22.000329738618735	27.142983965402813
21	28.21336625082963	21.718360945419803	21.66805466897202	28.400218134778548
22	28.69360095708748	22.428989942972127	20.69025284188188	28.187156258058515
23	28.64879032428525	21.649454028940905	21.649454028940905	28.052301617832942
24	28.2260485053963	21.585197272469784	21.8333467201576	28.35540750197632
25	28.51266745860301	21.52263148327422	22.17957226982765	27.785128788295125
26	28.39937265114077	22.05824536780652	21.90859476391983	27.633787217132884
27	27.390287929452846	21.66805466897202	23.127359427776675	27.814297973798464
28	26.890607099526104	22.401088982925458	21.645226610752015	29.06307730679642
29	27.628714315306212	22.93628012563887	22.188449848024316	27.246555711030602
30	27.015315936098343	21.836728654708708	23.377199842740044	27.770755566452898
31	28.208716090821852	22.0705048805543	22.13137970247431	27.58939932614954
32	26.06372410177932	21.85532929473982	22.48014170305769	29.600804900423167
33	26.32920596404158	21.648608545303127	24.17956381499127	27.84262167566402
34	27.042371412507237	21.21698914821751	23.610553326766745	28.130086112508508
35	26.383316916859368	22.50085605218325	23.96354274553902	27.152284285418364
36	26.51056220434494	21.334088632049745	24.512261626456873	27.643087537148435
37	26.456451251527152	20.78579249295078	24.759142848688022	27.998613406834043
38	25.66719227566149	20.203677008340694	25.82233852319373	28.306792192804085
39	24.834813634269143	19.4689517271117	27.42072534041285	28.27550929820631
40	24.794230419655804	20.100950746350684	28.017214046865156	27.087604787128356
41	23.19880279516891	19.5378586435906	29.030526186741973	28.232812374498522
42	21.863361389298714	20.368123575888497	29.966053831943217	27.802461202869573
43	21.126099657156384	20.623882376316313	29.773283562529855	28.476734403997444
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	4.0
4	7.0
5	7.0
6	10.5
7	14.0
8	9.5
9	5.0
10	13.0
11	21.0
12	21.0
13	48.0
14	75.0
15	103.5
16	132.0
17	136.5
18	141.0
19	141.0
20	175.5
21	210.0
22	215.0
23	220.0
24	281.0
25	342.0
26	342.0
27	442.5
28	543.0
29	739.5
30	936.0
31	1232.5
32	1529.0
33	1529.0
34	2028.0
35	2527.0
36	2992.5
37	3458.0
38	4574.5
39	5691.0
40	5691.0
41	6625.0
42	7559.0
43	9023.0
44	10487.0
45	13086.5
46	15686.0
47	15686.0
48	17734.0
49	19782.0
50	21875.0
51	23968.0
52	24807.0
53	25646.0
54	25646.0
55	24233.0
56	22820.0
57	22286.0
58	21752.0
59	20799.0
60	19846.0
61	19846.0
62	18074.0
63	16302.0
64	13910.0
65	11518.0
66	9930.5
67	8343.0
68	8343.0
69	7230.5
70	6118.0
71	5118.0
72	4118.0
73	3338.5
74	2559.0
75	2559.0
76	2068.0
77	1577.0
78	1321.5
79	1066.0
80	853.0
81	640.0
82	640.0
83	553.0
84	466.0
85	355.0
86	244.0
87	181.5
88	119.0
89	119.0
90	88.5
91	58.0
92	36.5
93	15.0
94	11.5
95	8.0
96	8.0
97	4.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	236551.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.967673167767146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.88181697474504	56.21595345630021
2	6.1895293388648165	7.918595796216208
3	1.9632691046903725	3.767572692876257
4	0.9972944711149303	2.5517842712120427
5	0.5902466709617639	1.887835306822235
6	0.40963265713460345	1.57219487582582
7	0.27903234058764953	1.2494334696163498
8	0.22387955235969423	1.1456843227432747
9	0.17084998918686234	0.9835988642119585
>10	1.1613635346150584	13.90575478192621
>50	0.09335839089170006	4.1595898477191495
>100	0.039064858600073075	4.425996732376043
>500	6.621162474588657E-4	0.21600558215423746
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	510	0.21559832763336445	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	386	0.16317834209113466	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	338	0.14288673478446506	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	318	0.1344318984066861	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	285	0.12048141838335075	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	269	0.11371754928112754	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	269	0.11371754928112754	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	260	0.10991287291112699	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	252	0.10653093836001537	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	251	0.10610819654112644	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	244	0.1031490038089038	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	242	0.10230352017112589	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	237	0.10018981107668112	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	8.454836377778999E-4	0.0
17	0.0	0.0	0.0	0.00211370909444475	0.0
18	4.2274181888894995E-4	0.0	0.0	0.00211370909444475	0.0
19	4.2274181888894995E-4	0.0	0.0	0.0025364509133337	0.0
20	4.2274181888894995E-4	0.0	0.0	0.0033819345511115996	0.0
21	4.2274181888894995E-4	0.0	0.0	0.005918385464445299	0.0
22	4.2274181888894995E-4	0.0	0.0	0.007186610921112149	0.0
23	4.2274181888894995E-4	0.0	0.0	0.008032094558890049	0.0
24	4.2274181888894995E-4	0.0	0.0	0.010991287291112699	0.0
25	4.2274181888894995E-4	0.0	0.0	0.012259512747779549	0.0
26	4.2274181888894995E-4	0.0	0.0	0.015641447298891147	0.0
27	4.2274181888894995E-4	0.0	0.0	0.04142869825111709	0.0
28	4.2274181888894995E-4	0.0	0.0	0.15218705480002198	0.0
29	4.2274181888894995E-4	0.0	0.0	0.3221292659933799	0.0
30	4.2274181888894995E-4	0.0	0.0	0.5440687209100786	0.0
31	4.2274181888894995E-4	0.0	0.0	1.1452075873701655	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACTG	25	0.005487633	29.599998	34
GTGTAAA	25	0.005487633	29.599998	1
ACTAACT	25	0.005487633	29.599998	33
GGTATCA	240	0.0	29.291666	1
ACTACTA	35	8.8485E-4	26.428572	32
ATACGGC	45	1.3188765E-4	24.666668	29
GTAACGT	145	0.0	24.24138	26
ATACACA	40	0.0019266806	23.125002	37
TGCGGGT	150	0.0	22.2	21
CGTCAAT	160	0.0	21.968752	30
TGAGCAA	145	0.0	21.689657	36
TCAATGA	155	0.0	21.483871	32
GTATCAA	335	0.0	20.985075	2
CGGGTAA	160	0.0	20.8125	23
TAAACCA	45	0.0038169653	20.555557	5
TTATACA	135	1.8189894E-12	20.555553	37
AACGTCA	165	0.0	20.181818	28
GGTAACG	165	0.0	20.181818	25
TATACAC	55	5.1265146E-4	20.181818	37
TAACGTC	175	0.0	20.085714	27
>>END_MODULE