FastQCFastQC Report
Fri 10 Feb 2017
ERR1632614.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632614.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388024
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11210.2888996556914005No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8160.21029627033379378No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5850.15076387027606541No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT5600.1443209698369173No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5530.14251695771395584No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT5450.14045522957342843No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC5160.13298146506401667No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5030.12963115683565965No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4470.11519905985196792No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4340.11184875162361091No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC4320.11133331958847906No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4270.11004473950064944No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4220.10875615941281981No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4170.10746757932499021No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC4090.10540585118446281No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4080.10514813516689689No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3920.10102467888584211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5900.031.669491
CTCGTAC358.858764E-426.4285723
GTATCAA7550.024.258282
CTTTATC400.001928894523.1250022
ATACGGC502.6976352E-422.19999929
CTTATAC4750.020.64210537
ACGGGTA450.0038213120.5555553
TGCACCG656.88708E-519.9230775
GCGGTAA3900.018.9743623
GGTAATA3950.018.73417725
CTACGGG609.2217885E-418.511
GTTCAAA701.2165575E-418.51
GTGGTAA701.2165575E-418.51
ATATTGA500.00702669318.4999983
AACTCCG4400.018.0795465
CGCGGTA4100.018.04878222
GTAATAC4100.018.04878226
TAATACG3700.018.027
CGTGCCA4250.017.8470610
TAGGACA953.5939447E-617.5263164
Produced by FastQC (version 0.11.5)