##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632614.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	388024
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.036943591118074	34.0	33.0	34.0	31.0	34.0
2	33.17232181514545	34.0	33.0	34.0	31.0	34.0
3	33.250121126528256	34.0	34.0	34.0	31.0	34.0
4	36.53463960992104	37.0	37.0	37.0	35.0	37.0
5	36.50599447456858	37.0	37.0	37.0	35.0	37.0
6	36.548378450817474	37.0	37.0	37.0	35.0	37.0
7	36.54909747850648	37.0	37.0	37.0	35.0	37.0
8	36.52224604663629	37.0	37.0	37.0	35.0	37.0
9	38.31846999154691	39.0	39.0	39.0	37.0	39.0
10	38.34913046615673	39.0	39.0	39.0	37.0	39.0
11	38.41739170772942	39.0	39.0	39.0	37.0	39.0
12	38.379368286497744	39.0	39.0	39.0	37.0	39.0
13	38.41275539657341	39.0	39.0	39.0	37.0	39.0
14	39.96033750489661	41.0	40.0	41.0	38.0	41.0
15	39.960605529554876	41.0	40.0	41.0	38.0	41.0
16	39.920667278312685	41.0	40.0	41.0	38.0	41.0
17	39.92332433045379	41.0	40.0	41.0	38.0	41.0
18	39.92083994804445	41.0	40.0	41.0	38.0	41.0
19	39.92772869719399	41.0	40.0	41.0	38.0	41.0
20	39.89862998165062	41.0	40.0	41.0	38.0	41.0
21	39.86310382862916	41.0	40.0	41.0	38.0	41.0
22	39.83078366286622	41.0	40.0	41.0	38.0	41.0
23	39.78902335937983	41.0	40.0	41.0	38.0	41.0
24	39.77460414819702	41.0	40.0	41.0	38.0	41.0
25	39.73842855081129	41.0	40.0	41.0	38.0	41.0
26	39.64534667958683	41.0	40.0	41.0	37.0	41.0
27	39.54938612044616	41.0	40.0	41.0	37.0	41.0
28	39.50993752963734	41.0	40.0	41.0	37.0	41.0
29	39.46027307589221	41.0	39.0	41.0	37.0	41.0
30	39.42231923798528	41.0	39.0	41.0	37.0	41.0
31	39.357013999134075	41.0	39.0	41.0	36.0	41.0
32	39.30535997773334	41.0	39.0	41.0	36.0	41.0
33	39.243871513102285	41.0	39.0	41.0	36.0	41.0
34	39.200142259241694	41.0	39.0	41.0	35.0	41.0
35	39.11316568047337	41.0	39.0	41.0	35.0	41.0
36	39.06178226089108	41.0	39.0	41.0	35.0	41.0
37	39.008172174917014	41.0	39.0	41.0	35.0	41.0
38	38.927927138527515	40.0	39.0	41.0	35.0	41.0
39	38.82477630249675	40.0	38.0	41.0	35.0	41.0
40	38.755749644351894	40.0	38.0	41.0	35.0	41.0
41	38.69574562398202	40.0	38.0	41.0	35.0	41.0
42	38.62404902789518	40.0	38.0	41.0	35.0	41.0
43	37.88782394903408	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	2.0
19	13.0
20	10.0
21	41.0
22	96.0
23	142.0
24	235.0
25	349.0
26	470.0
27	673.0
28	879.0
29	1220.0
30	1742.0
31	2199.0
32	2954.0
33	3876.0
34	6025.0
35	9625.0
36	15357.0
37	29730.0
38	77179.0
39	235204.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.05506875863349	16.280436261674534	12.561594128198255	28.10290085149372
2	21.71747108426283	19.471475991175804	31.638506896480628	27.172546028080735
3	21.558202585407088	19.293651939055316	28.61317856627425	30.534966909263346
4	16.13920788404841	15.566820609034494	33.148465043399376	35.14550646351772
5	17.6911737418304	32.78199286642064	32.31887718285466	17.207956208894295
6	34.867430880564086	33.22011009628271	14.964795992000496	16.94766303115271
7	30.81149619611158	27.25785002989506	19.11247757870647	22.818176195286892
8	27.66787621384244	30.436003958518032	19.817073170731707	22.07904665690782
9	28.084860730264104	12.776013854813103	16.95178648743377	42.18733892748902
10	19.790012988887284	23.038523390305755	29.11907510875616	28.0523885120508
11	39.86763705337814	19.26556089314063	18.463033214440344	22.403768839040882
12	23.1266622683133	23.391594334371067	26.245026080860978	27.23671731645465
13	35.91994309630332	17.1602787456446	20.24307774776818	26.6767004102839
14	24.540750056697526	19.30112570356473	22.491134568995733	33.66698967074201
15	29.713368245263176	23.794662185844174	19.289528482774262	27.202441086118384
16	26.272601694740533	23.570964682596955	21.6821639898563	28.47426963280622
17	27.447013586788444	23.262994041605676	21.272653237944045	28.017339133661835
18	27.051934931859883	20.873708842751995	22.750139166649486	29.324217058738633
19	29.05413067232955	21.86849267055646	22.42361297239346	26.653763684720534
20	30.33807187184298	20.602849308290207	21.87545100303074	27.18362781683607
21	28.165783559779804	21.658969548275365	21.25719027689009	28.918056615054738
22	28.52478197224914	22.57721171886275	20.68557614992887	28.21243015895924
23	28.719872997546542	21.59402511184875	21.69917324701565	27.986928643589053
24	28.255984165927885	21.21105910974579	21.928025070614186	28.60493165371214
25	29.026039626414864	21.57340783044348	21.99477351916376	27.4057790239779
26	28.51395789951137	22.13909448900068	21.76540626353009	27.58154134795786
27	27.68127744675587	21.458981918644206	23.26067459744758	27.59906603715234
28	27.018431849576313	22.793177741582994	21.452023586169926	28.73636682267076
29	27.46453827598293	23.288250211327135	21.54042018019504	27.706791332494895
30	27.327175638620293	22.10378739459415	23.112487887347175	27.456549079438386
31	28.264231078489992	21.975702533863885	22.002504999690743	27.75756138795538
32	25.749438179081707	21.922097602210172	22.664062016782466	29.664402201925654
33	26.559439622291404	21.631909366430943	23.656268684411273	28.15238232686638
34	26.83081458878832	21.36259612807455	23.473032595921904	28.33355668721522
35	26.269766818547307	22.508143826155084	24.014751664845473	27.20733769045214
36	26.45686864730017	21.34610230295033	24.70852318413294	27.48850586561656
37	27.03131765045461	20.55929530132157	24.365760880770264	28.04362616745356
38	25.49249530956848	20.008813887800756	26.199668061769376	28.29902274086139
39	24.997938271859475	19.224584046347648	27.206049110364305	28.57142857142857
40	24.80774385089582	19.801352493660186	28.43922025441725	26.951683401026738
41	23.165577386965754	19.502401913283716	29.251283425767475	28.080737273983054
42	21.674947941364454	20.137929612601283	30.26797311506505	27.91914933096922
43	20.78556996474445	20.522957342844773	29.81258891202606	28.87888378038472
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	3.5
2	4.0
3	10.5
4	17.0
5	17.0
6	16.5
7	16.0
8	15.0
9	14.0
10	24.5
11	35.0
12	35.0
13	66.0
14	97.0
15	165.5
16	234.0
17	259.0
18	284.0
19	284.0
20	355.0
21	426.0
22	437.0
23	448.0
24	566.0
25	684.0
26	684.0
27	847.5
28	1011.0
29	1482.5
30	1954.0
31	2290.5
32	2627.0
33	2627.0
34	3347.0
35	4067.0
36	4659.5
37	5252.0
38	6556.0
39	7860.0
40	7860.0
41	9777.0
42	11694.0
43	13676.5
44	15659.0
45	20583.0
46	25507.0
47	25507.0
48	28940.5
49	32374.0
50	36107.0
51	39840.0
52	42094.0
53	44348.0
54	44348.0
55	41631.5
56	38915.0
57	37244.0
58	35573.0
59	34077.5
60	32582.0
61	32582.0
62	29810.0
63	27038.0
64	22733.0
65	18428.0
66	16297.0
67	14166.0
68	14166.0
69	11987.5
70	9809.0
71	8303.5
72	6798.0
73	5449.5
74	4101.0
75	4101.0
76	3342.0
77	2583.0
78	2079.0
79	1575.0
80	1256.0
81	937.0
82	937.0
83	739.5
84	542.0
85	413.0
86	284.0
87	205.5
88	127.0
89	127.0
90	100.5
91	74.0
92	48.0
93	22.0
94	16.5
95	11.0
96	11.0
97	7.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	388024.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.43466810759994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.11439462699936	49.162719481691965
2	6.35542422200693	7.1733251330392545
3	2.027931001092624	3.433368389753254
4	1.0141992603266214	2.2894399460602495
5	0.6300219755770489	1.7777540546092592
6	0.44339901866369436	1.501384587451266
7	0.33266803421384	1.3141807070606035
8	0.24832596465124754	1.12113547180742
9	0.20152934467453004	1.0235917512584296
>10	1.4089376311516144	15.44812430851298
>50	0.13106018748207315	5.08614626009005
>100	0.08844271393019094	9.324299761403191
>500	0.0032077668264836093	1.0546247240882598
>1k	4.5825240378337276E-4	0.2899054231738448
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1121	0.2888996556914005	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	816	0.21029627033379378	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	585	0.15076387027606541	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	560	0.1443209698369173	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	553	0.14251695771395584	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	545	0.14045522957342843	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	516	0.13298146506401667	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	503	0.12963115683565965	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	447	0.11519905985196792	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	434	0.11184875162361091	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	432	0.11133331958847906	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	427	0.11004473950064944	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	422	0.10875615941281981	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	417	0.10746757932499021	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	409	0.10540585118446281	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	408	0.10514813516689689	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	392	0.10102467888584211	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.5771601756592377E-4	0.0	0.0	0.0	0.0
6	2.5771601756592377E-4	0.0	0.0	0.0	0.0
7	2.5771601756592377E-4	0.0	0.0	0.0	0.0
8	2.5771601756592377E-4	0.0	0.0	0.0	0.0
9	2.5771601756592377E-4	0.0	0.0	0.0	0.0
10	2.5771601756592377E-4	0.0	0.0	0.0	0.0
11	2.5771601756592377E-4	0.0	0.0	0.0	0.0
12	2.5771601756592377E-4	0.0	0.0	0.0	0.0
13	2.5771601756592377E-4	0.0	0.0	0.0	0.0
14	2.5771601756592377E-4	0.0	0.0	0.0	0.0
15	2.5771601756592377E-4	0.0	0.0	0.0	0.0
16	2.5771601756592377E-4	0.0	0.0	5.154320351318475E-4	0.0
17	2.5771601756592377E-4	0.0	0.0	0.0015462961053955425	0.0
18	2.5771601756592377E-4	0.0	0.0	0.0015462961053955425	0.0
19	2.5771601756592377E-4	0.0	0.0	0.0015462961053955425	0.0
20	2.5771601756592377E-4	0.0	0.0	0.0025771601756592374	0.0
21	2.5771601756592377E-4	0.0	2.5771601756592377E-4	0.003350308228357009	0.0
22	2.5771601756592377E-4	0.0	2.5771601756592377E-4	0.006700616456714018	0.0
23	2.5771601756592377E-4	0.0	2.5771601756592377E-4	0.007731480526977713	0.0
24	2.5771601756592377E-4	0.0	2.5771601756592377E-4	0.010824072737768798	0.0
25	5.154320351318475E-4	0.0	2.5771601756592377E-4	0.012628084860730264	0.0
26	5.154320351318475E-4	0.0	2.5771601756592377E-4	0.018555553264746512	0.0
27	5.154320351318475E-4	0.0	2.5771601756592377E-4	0.04278085891594335	0.0
28	5.154320351318475E-4	0.0	2.5771601756592377E-4	0.16957713955837783	0.0
29	5.154320351318475E-4	0.0	2.5771601756592377E-4	0.36286415273282063	0.0
30	5.154320351318475E-4	0.0	2.5771601756592377E-4	0.6136218378244644	0.0
31	5.154320351318475E-4	0.0	2.5771601756592377E-4	1.267447374389213	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	590	0.0	31.66949	1
CTCGTAC	35	8.858764E-4	26.428572	3
GTATCAA	755	0.0	24.25828	2
CTTTATC	40	0.0019288945	23.125002	2
ATACGGC	50	2.6976352E-4	22.199999	29
CTTATAC	475	0.0	20.642105	37
ACGGGTA	45	0.00382131	20.555555	3
TGCACCG	65	6.88708E-5	19.923077	5
GCGGTAA	390	0.0	18.97436	23
GGTAATA	395	0.0	18.734177	25
CTACGGG	60	9.2217885E-4	18.5	11
GTTCAAA	70	1.2165575E-4	18.5	1
GTGGTAA	70	1.2165575E-4	18.5	1
ATATTGA	50	0.007026693	18.499998	3
AACTCCG	440	0.0	18.079546	5
CGCGGTA	410	0.0	18.048782	22
GTAATAC	410	0.0	18.048782	26
TAATACG	370	0.0	18.0	27
CGTGCCA	425	0.0	17.84706	10
TAGGACA	95	3.5939447E-6	17.526316	4
>>END_MODULE