FastQCFastQC Report
Fri 10 Feb 2017
ERR1632613.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632613.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56969
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3600.6319226245853008No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2290.4019730028612052No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1840.32298267478804266No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1840.32298267478804266No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1550.27207779669644894No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1540.27032245607260086No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1530.26856711544875284No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1470.2580350717056645No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1450.2545243904579684No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1410.24750302796257614No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT1380.242237006091032RNA PCR Primer, Index 26 (100% over 22bp)
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1360.23872632484333586No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1320.23170496234794363No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1320.23170496234794363No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1310.22994962172409555No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1310.22994962172409555No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1250.2194175779810072No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1150.2018641717425266No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1120.19659814987098245No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1100.19308746862328635No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1100.19308746862328635No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1070.18782144675174217No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1070.18782144675174217No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1060.18606610612789412No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1010.1772894030086538No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC990.1737787217609577No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA980.17202338113710966No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA950.16675735926556548No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA910.15973599677017325No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA910.15973599677017325No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA880.15446997489862907No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC870.15271463427478102No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG860.15095929365093297No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG860.15095929365093297No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA850.14920395302708492No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA830.1456932717793888No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA810.14218259053169266No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC810.14218259053169266No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA790.13867190928399656No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC780.1369165686601485No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC770.13516122803630043No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC760.13340588741245238No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG750.13165054678860433No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC740.12989520616475625No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC740.12989520616475625No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC740.12989520616475625No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG740.12989520616475625No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA730.1281398655409082No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG720.12638452491706015No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT720.12638452491706015No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT710.12462918429321208No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA700.12287384366936405No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG700.12287384366936405No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA700.12287384366936405No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC680.11936316242166793No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG680.11936316242166793No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA670.11760782179781988No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG670.11760782179781988No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC660.11585248117397182No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG650.11409714055012375No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC650.11409714055012375No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT640.1123417999262757No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC610.10707577805473152No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA600.10532043743088347No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC600.10532043743088347No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA590.1035650968070354No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT580.10180975618318736No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC580.10180975618318736No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA570.10005441555933929No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA570.10005441555933929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGGGC200.001827287337.06
GGTATCA1000.031.451
AGTCTGT303.5570687E-430.83333237
TACGGCT250.0054531329.630
ATACGGC250.0054531329.629
ATGCCGT250.0054531329.634
TCTCCGA250.0054531329.61
CGGAGCT556.0879756E-726.9090932
GCCGTCT358.7658776E-426.4285736
CGCGGTA1250.025.16000222
GCGGTAA1300.024.19230823
CGTGCCA1300.024.19230810
CCGCGGT1300.024.19230821
GCTCACA551.8618353E-523.54545431
AACTCCG1350.023.2962975
GTGCCAG1350.023.29629711
GGTCCCC400.001908860923.12523
CCCCCCA400.001908860923.12526
CCCCATA400.001908860923.12528
CTCCGTG1400.022.4642857
Produced by FastQC (version 0.11.5)