FastQCFastQC Report
Fri 10 Feb 2017
ERR1632612.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632612.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90765
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3470.38230595493857766No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2290.2522998953341046No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1870.2060265520850548No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1860.20492480581722028No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1580.1740759103178538No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1540.16966892524651572No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1500.16526194017517765No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1380.15204098496116344No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1360.1498374924254944No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT1270.13992177601498376TruSeq Adapter, Index 12 (95% over 22bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1250.13771828347931472No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1240.1366165372114802No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1190.1311078058723076No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1170.12890431333663857No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1110.12229383572963147No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1110.12229383572963147No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1110.12229383572963147No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1110.12229383572963147No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1110.12229383572963147No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1100.12119208946179695No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1090.12009034319396245No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1050.11568335812262437No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1020.11237811931912081No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1000.11017462678345176No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC950.10466589544427918No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC950.10466589544427918No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC920.10136065664077562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1500.032.0666661
GCCGTCT250.00547003729.636
CGCGGTA1250.026.64000122
TAATACG1300.025.61538527
GCGGTAA1300.025.61538523
ATACGGA1250.025.16000229
GTATCAA1950.024.6666682
AATACGG1350.024.66666828
TACGGAG1300.024.19230830
GGTAATA1400.023.78571325
CCGCGGT1400.023.78571321
GTGCCAG1400.023.78571311
ACGGAGG1103.6379788E-1223.54545431
GTAACGT551.8777495E-523.54545426
GTGCAGC400.001917587623.1251
GTAATAC1450.022.96551726
CGGTAAT1450.022.96551724
GCAGCCG1400.022.46428517
GCCAGCA1500.022.213
AGCCGCG1450.021.68965519
Produced by FastQC (version 0.11.5)