##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632612.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	90765
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07794854844929	34.0	33.0	34.0	31.0	34.0
2	33.221065388640994	34.0	33.0	34.0	31.0	34.0
3	33.290310141574395	34.0	34.0	34.0	31.0	34.0
4	36.5652509227125	37.0	37.0	37.0	35.0	37.0
5	36.53117391064838	37.0	37.0	37.0	35.0	37.0
6	36.57637856001763	37.0	37.0	37.0	35.0	37.0
7	36.58063130061147	37.0	37.0	37.0	35.0	37.0
8	36.54733652839751	37.0	37.0	37.0	35.0	37.0
9	38.35239354376687	39.0	39.0	39.0	37.0	39.0
10	38.39113094254393	39.0	39.0	39.0	37.0	39.0
11	38.46057400980554	39.0	39.0	39.0	37.0	39.0
12	38.41915936759764	39.0	39.0	39.0	37.0	39.0
13	38.44350796011678	39.0	39.0	39.0	37.0	39.0
14	40.0040213738776	41.0	40.0	41.0	38.0	41.0
15	40.013441304467584	41.0	40.0	41.0	38.0	41.0
16	39.9797499035972	41.0	40.0	41.0	38.0	41.0
17	39.97854900016526	41.0	40.0	41.0	38.0	41.0
18	39.9758827741971	41.0	40.0	41.0	38.0	41.0
19	39.97306230375145	41.0	40.0	41.0	38.0	41.0
20	39.95244863108026	41.0	40.0	41.0	38.0	41.0
21	39.90271580455021	41.0	40.0	41.0	38.0	41.0
22	39.88790833471052	41.0	40.0	41.0	38.0	41.0
23	39.855968710405996	41.0	40.0	41.0	38.0	41.0
24	39.82658513744285	41.0	40.0	41.0	38.0	41.0
25	39.78816724508346	41.0	40.0	41.0	38.0	41.0
26	39.70888558365009	41.0	40.0	41.0	38.0	41.0
27	39.605442626563104	41.0	40.0	41.0	37.0	41.0
28	39.58142455792431	41.0	40.0	41.0	37.0	41.0
29	39.521687875282325	41.0	40.0	41.0	37.0	41.0
30	39.481319892028864	41.0	40.0	41.0	37.0	41.0
31	39.42922932848565	41.0	39.0	41.0	37.0	41.0
32	39.37158596375255	41.0	39.0	41.0	36.0	41.0
33	39.2979893130612	41.0	39.0	41.0	36.0	41.0
34	39.263680934280835	41.0	39.0	41.0	36.0	41.0
35	39.19721258194238	41.0	39.0	41.0	35.0	41.0
36	39.14213628601333	41.0	39.0	41.0	35.0	41.0
37	39.08170550322261	41.0	39.0	41.0	35.0	41.0
38	39.00462733432491	41.0	39.0	41.0	35.0	41.0
39	38.88811766650141	40.0	39.0	41.0	35.0	41.0
40	38.836489836390676	40.0	38.0	41.0	35.0	41.0
41	38.77750234121082	40.0	38.0	41.0	35.0	41.0
42	38.69567564589875	40.0	38.0	41.0	35.0	41.0
43	37.98027874180576	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	6.0
20	6.0
21	12.0
22	17.0
23	36.0
24	50.0
25	96.0
26	114.0
27	140.0
28	202.0
29	286.0
30	366.0
31	484.0
32	678.0
33	849.0
34	1301.0
35	2017.0
36	3429.0
37	6627.0
38	16970.0
39	57077.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.608935162232136	16.30364127141519	12.745000826309703	28.342422740042966
2	21.68016305844764	19.762022806147744	31.472483886960834	27.085330248443785
3	21.493967939183605	19.128518702142898	28.919737784388253	30.45777557428524
4	15.859637525477883	15.686663361427863	33.67487467636203	34.77882443673222
5	17.505646449622652	32.7450008263097	32.67118382636479	17.07816889770286
6	34.170660496887564	33.58893846747094	15.364953451220185	16.87544758442131
7	30.049027708918636	27.12829835288933	19.867790447859857	22.954883490332175
8	27.093042472318622	30.529389081694486	20.384509447474247	21.993058998512645
9	27.790447859857874	12.666776841293451	17.348096733322315	42.19467856552636
10	19.376411612405665	22.998953341045556	29.14779926183	28.47683578471878
11	39.996694761196494	19.21004792596265	18.279072329642485	22.51418498319837
12	22.790723296424833	23.485925191428414	26.452927890706768	27.27042362143998
13	35.411226794469236	17.096898584256046	21.113865476780695	26.378009144494026
14	24.71216878752823	19.396243045226687	22.4480802071283	33.44350796011678
15	30.01377182834793	23.70407095245965	19.311408582603427	26.970748636588993
16	25.575937861510496	24.012559907453312	22.064672505921887	28.346829725114308
17	27.52823224811326	23.445160579518536	21.341926954222444	27.684680218145765
18	26.568611248829395	21.281330909491544	23.02870049027709	29.121357351401972
19	28.54734754586019	22.210103013276044	22.836996639673885	26.405552801189884
20	30.20547567895114	20.60706219357682	22.203492535669035	26.98396959180301
21	27.938081859747697	21.40692998402468	21.59422684955655	29.060761306671072
22	28.426155456398387	22.507574505591364	21.013606566407756	28.05266347160249
23	28.70489726216052	21.488459207844436	21.63609320773426	28.170550322260784
24	28.353440202721313	21.118272461852037	22.374263207183386	28.154024128243265
25	29.002368754475842	21.66363686443012	22.294937475899303	27.039056905194737
26	28.883380157549716	22.633173580124495	21.716520685286177	26.766925577039608
27	27.37839475568776	21.703299730072164	23.923318459758715	26.99498705448135
28	26.817605905359997	22.908610147083127	21.80796562551644	28.46581832204043
29	27.17787693494188	23.5366055197488	21.514901118272462	27.770616427036853
30	26.988376576874344	22.34121081914835	23.48041646008924	27.189996143888063
31	28.40852751611304	22.251969371453754	22.064672505921887	27.27483060651132
32	25.754420756899687	21.8288988046053	22.806147744174517	29.610532694320497
33	26.278851980388918	21.945683908995758	23.828568280724948	27.946895829890376
34	26.944306726160967	21.779320222552744	23.315154519914064	27.96121853137222
35	26.539965845865698	22.786316311353495	24.0312895940065	26.64242824877431
36	26.654547457720483	21.57329367046769	24.494023026496997	27.27813584531482
37	27.111772158871812	21.123781193191206	24.26045281771608	27.503993830220903
38	26.371398666887014	20.79325731284085	25.543987219743293	27.29135680052884
39	25.24651572742797	19.713545970363025	26.516829174241174	28.523109127967828
40	25.098881727538146	20.486971850382858	27.56018288988046	26.853963532198534
41	23.25125323637966	20.0	28.566077232413374	28.18266953120696
42	22.293835729631468	20.59494298463064	29.158816724508345	27.95240456122955
43	21.343028700490276	21.369470610918306	28.983639067922656	28.30386162066876
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	3.5
4	5.0
5	5.0
6	5.0
7	5.0
8	5.0
9	5.0
10	5.5
11	6.0
12	6.0
13	18.0
14	30.0
15	40.0
16	50.0
17	58.5
18	67.0
19	67.0
20	86.5
21	106.0
22	97.5
23	89.0
24	113.0
25	137.0
26	137.0
27	167.5
28	198.0
29	297.0
30	396.0
31	452.0
32	508.0
33	508.0
34	715.5
35	923.0
36	1066.5
37	1210.0
38	1542.0
39	1874.0
40	1874.0
41	2306.0
42	2738.0
43	3208.5
44	3679.0
45	4943.0
46	6207.0
47	6207.0
48	6893.5
49	7580.0
50	8813.5
51	10047.0
52	10505.5
53	10964.0
54	10964.0
55	10202.5
56	9441.0
57	8981.5
58	8522.0
59	7907.5
60	7293.0
61	7293.0
62	6787.0
63	6281.0
64	5108.5
65	3936.0
66	3421.5
67	2907.0
68	2907.0
69	2508.5
70	2110.0
71	1806.5
72	1503.0
73	1153.0
74	803.0
75	803.0
76	649.0
77	495.0
78	408.5
79	322.0
80	238.5
81	155.0
82	155.0
83	123.0
84	91.0
85	71.0
86	51.0
87	33.0
88	15.0
89	15.0
90	11.5
91	8.0
92	5.5
93	3.0
94	2.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	90765.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.337905580344845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.09814248599353	54.29515782515286
2	5.892437390643502	7.346444113920564
3	2.1685724889980733	4.0555280118988595
4	1.1859104646436083	2.9570869828678457
5	0.7352291405242043	2.2916322370957967
6	0.5355154557183507	2.002974714923153
7	0.4047295028366413	1.766099267338732
8	0.33226701542920767	1.6570263868231145
9	0.24213075060532688	1.3584531482399602
>10	1.2742793517258444	14.853743182944967
>50	0.0901362648238808	3.8043298628325894
>100	0.0406496880578286	3.6115242659615494
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	347	0.38230595493857766	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	229	0.2522998953341046	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	187	0.2060265520850548	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	186	0.20492480581722028	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	158	0.1740759103178538	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	154	0.16966892524651572	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	150	0.16526194017517765	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	138	0.15204098496116344	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	136	0.1498374924254944	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	127	0.13992177601498376	TruSeq Adapter, Index 12 (95% over 22bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	125	0.13771828347931472	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	124	0.1366165372114802	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	119	0.1311078058723076	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	117	0.12890431333663857	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	111	0.12229383572963147	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	111	0.12229383572963147	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	111	0.12229383572963147	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	111	0.12229383572963147	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	111	0.12229383572963147	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	110	0.12119208946179695	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	109	0.12009034319396245	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	105	0.11568335812262437	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	102	0.11237811931912081	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	100	0.11017462678345176	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	95	0.10466589544427918	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	95	0.10466589544427918	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	92	0.10136065664077562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0011017462678345176	0.0	0.0	0.0	0.0
9	0.0011017462678345176	0.0	0.0	0.0	0.0
10	0.0011017462678345176	0.0	0.0	0.0	0.0
11	0.0011017462678345176	0.0	0.0	0.0	0.0
12	0.0011017462678345176	0.0	0.0	0.0	0.0
13	0.0011017462678345176	0.0	0.0	0.0	0.0
14	0.0011017462678345176	0.0	0.0	0.0011017462678345176	0.0
15	0.0011017462678345176	0.0	0.0	0.0011017462678345176	0.0
16	0.0011017462678345176	0.0	0.0	0.0011017462678345176	0.0
17	0.0011017462678345176	0.0	0.0	0.0011017462678345176	0.0
18	0.0011017462678345176	0.0	0.0	0.0011017462678345176	0.0
19	0.0011017462678345176	0.0	0.0	0.0011017462678345176	0.0
20	0.0011017462678345176	0.0	0.0	0.0011017462678345176	0.0
21	0.0011017462678345176	0.0	0.0	0.002203492535669035	0.0
22	0.0011017462678345176	0.0	0.0	0.003305238803503553	0.0
23	0.0011017462678345176	0.0	0.0	0.00440698507133807	0.0
24	0.0011017462678345176	0.0	0.0	0.00440698507133807	0.0
25	0.0011017462678345176	0.0	0.0	0.005508731339172589	0.0
26	0.0011017462678345176	0.0	0.0	0.00991571641051066	0.0
27	0.0011017462678345176	0.0	0.0	0.03195064176720101	0.0
28	0.0011017462678345176	0.0	0.0	0.11127637305128629	0.0
29	0.0011017462678345176	0.0	0.0	0.2556051341376081	0.0
30	0.0011017462678345176	0.0	0.0	0.43849501459813806	0.0
31	0.0011017462678345176	0.0	0.0	0.9375860739271745	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	150	0.0	32.066666	1
GCCGTCT	25	0.005470037	29.6	36
CGCGGTA	125	0.0	26.640001	22
TAATACG	130	0.0	25.615385	27
GCGGTAA	130	0.0	25.615385	23
ATACGGA	125	0.0	25.160002	29
GTATCAA	195	0.0	24.666668	2
AATACGG	135	0.0	24.666668	28
TACGGAG	130	0.0	24.192308	30
GGTAATA	140	0.0	23.785713	25
CCGCGGT	140	0.0	23.785713	21
GTGCCAG	140	0.0	23.785713	11
ACGGAGG	110	3.6379788E-12	23.545454	31
GTAACGT	55	1.8777495E-5	23.545454	26
GTGCAGC	40	0.0019175876	23.125	1
GTAATAC	145	0.0	22.965517	26
CGGTAAT	145	0.0	22.965517	24
GCAGCCG	140	0.0	22.464285	17
GCCAGCA	150	0.0	22.2	13
AGCCGCG	145	0.0	21.689655	19
>>END_MODULE