FastQCFastQC Report
Fri 10 Feb 2017
ERR1632611.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632611.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70938
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3270.4609659138966421No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2360.3326848797541515No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2050.2889847472440723No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1980.2791169753869576No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1950.2748879303053371No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1780.2509233415094872No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1740.24528461473399304No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1710.2410555696523725No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1690.23823620626462544No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1670.23541684287687842No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1560.21991034424426967No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1510.212861935774902No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1490.21004257238715499No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1480.20863289069328145No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1480.20863289069328145No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1450.20440384561166092No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1340.18889734697905214No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1320.1860779835913051No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1270.17902957512193746No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1210.1705714849586963No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1200.1691618032648228No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1190.16775212157094926No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1190.16775212157094926No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1130.15929403140770815No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1110.1564746680199611No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1030.1451972144689729No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1020.14378753277509937No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC990.1395584876934788No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT990.1395584876934788RNA PCR Primer, Index 17 (95% over 21bp)
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA980.1381488059996053No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC980.1381488059996053No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA970.13673912430573176No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC950.13391976091798471No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC940.1325100792241112No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT940.1325100792241112No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC930.13110039753023767No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC890.1254616707547436No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG880.12405198906087006No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA870.12264230736699652No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA860.12123262567312301No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC840.11841326228537595No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC830.11700358059150244No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA820.11559389889762892No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC790.11136485381600834No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT780.10995517212213483No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA780.10995517212213483No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT770.1085454904282613No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA740.10431644534664072No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC730.1029067636527672No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG730.1029067636527672No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG720.10149708195889369No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA710.10008740026502015No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG710.10008740026502015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1050.029.952381
TGGTCAG250.005462058429.59999830
GATAAAC250.005462058429.5999983
ATAAACT250.005462058429.5999984
GGTAGAG250.005462058429.59999836
AGGTAGA250.005462058429.59999835
GTCAGGT250.005462058429.59999832
AAATCGT358.787237E-426.428575
GACACAG358.787237E-426.4285722
CGTACCC358.787237E-426.428579
ACCCCAA358.787237E-426.4285712
TACCCCA358.787237E-426.4285711
CAAATCG358.787237E-426.428574
CCGACAC358.787237E-426.4285720
CCAAACC451.3061856E-424.66666615
CCCAAAC451.3061856E-424.66666614
CCCCAAA451.3061856E-424.66666613
AACCGAC400.001913467123.12500218
TCAAATC400.001913467123.1250023
TCGTACC400.001913467123.1250028
Produced by FastQC (version 0.11.5)