##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632611.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70938
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.049437537004145	34.0	33.0	34.0	31.0	34.0
2	33.192336970312105	34.0	33.0	34.0	31.0	34.0
3	33.253615833544785	34.0	34.0	34.0	31.0	34.0
4	36.532295807606644	37.0	37.0	37.0	35.0	37.0
5	36.50988186867405	37.0	37.0	37.0	35.0	37.0
6	36.55774056218106	37.0	37.0	37.0	35.0	37.0
7	36.5630691589839	37.0	37.0	37.0	35.0	37.0
8	36.52617778905523	37.0	37.0	37.0	35.0	37.0
9	38.33947954551862	39.0	39.0	39.0	37.0	39.0
10	38.371874030843834	39.0	39.0	39.0	37.0	39.0
11	38.435070061180184	39.0	39.0	39.0	37.0	39.0
12	38.383940906143394	39.0	39.0	39.0	37.0	39.0
13	38.432194310524686	39.0	39.0	39.0	37.0	39.0
14	39.96138881840481	41.0	40.0	41.0	38.0	41.0
15	39.976063604838025	41.0	40.0	41.0	38.0	41.0
16	39.92967098029265	41.0	40.0	41.0	38.0	41.0
17	39.933942315825085	41.0	40.0	41.0	38.0	41.0
18	39.94505060757281	41.0	40.0	41.0	38.0	41.0
19	39.94658716061913	41.0	40.0	41.0	38.0	41.0
20	39.93591587019651	41.0	40.0	41.0	38.0	41.0
21	39.904254419352114	41.0	40.0	41.0	38.0	41.0
22	39.87995150694973	41.0	40.0	41.0	38.0	41.0
23	39.84190419803208	41.0	40.0	41.0	38.0	41.0
24	39.825171276325804	41.0	40.0	41.0	38.0	41.0
25	39.79491950717528	41.0	40.0	41.0	38.0	41.0
26	39.702444388057174	41.0	40.0	41.0	38.0	41.0
27	39.586089261044854	41.0	40.0	41.0	37.0	41.0
28	39.56753784995348	41.0	40.0	41.0	37.0	41.0
29	39.53999266965519	41.0	40.0	41.0	37.0	41.0
30	39.48409879049311	41.0	40.0	41.0	37.0	41.0
31	39.41052750288985	41.0	39.0	41.0	36.0	41.0
32	39.35935605740224	41.0	39.0	41.0	36.0	41.0
33	39.28489667033184	41.0	39.0	41.0	36.0	41.0
34	39.259846626631706	41.0	39.0	41.0	36.0	41.0
35	39.177493022075616	41.0	39.0	41.0	35.0	41.0
36	39.12183878880149	41.0	39.0	41.0	35.0	41.0
37	39.057825143082695	41.0	39.0	41.0	35.0	41.0
38	38.986424765288	40.0	39.0	41.0	35.0	41.0
39	38.88399729341115	40.0	39.0	41.0	35.0	41.0
40	38.82824438241845	40.0	38.0	41.0	35.0	41.0
41	38.75316473540275	40.0	38.0	41.0	35.0	41.0
42	38.69380303927373	40.0	38.0	41.0	35.0	41.0
43	37.98054639262455	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	2.0
15	1.0
16	0.0
17	0.0
18	1.0
19	0.0
20	4.0
21	7.0
22	8.0
23	26.0
24	40.0
25	51.0
26	92.0
27	125.0
28	168.0
29	221.0
30	275.0
31	381.0
32	509.0
33	702.0
34	1029.0
35	1673.0
36	2665.0
37	5251.0
38	13782.0
39	43923.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.352476810736135	16.06755194677042	13.032507259860724	28.54746398263272
2	22.12072514026333	19.242155121373592	31.341453099890042	27.295666638473033
3	21.42575206518368	18.80938284135442	29.465166765344385	30.299698328117515
4	15.788434971383461	16.30860751642279	34.92486396571654	32.978093546477204
5	17.05150976909414	32.22109447686712	33.10496489892582	17.622430855112917
6	31.746031746031743	34.33279765428966	16.40023682652457	17.52093377315402
7	28.978826580958017	27.23927937071809	19.84408920465759	23.9378048436663
8	26.75011982294398	29.4059601342017	21.398968113000087	22.44495192985424
9	27.760861597451296	12.19092728861823	17.426485099664497	42.62172601426598
10	20.16549663086075	22.361780709915703	27.797513321492005	29.67520933773154
11	40.01663424398771	18.820660294905412	18.177845442499084	22.984860018607797
12	22.28565789844653	23.65445882319772	26.153824466435477	27.90605881192027
13	37.51585891905608	16.868251148890582	19.342242521638614	26.27364741041473
14	24.07031492289041	20.622233499675772	22.743804448955427	32.563647128478394
15	31.101807211931547	23.357015985790408	19.033522230680315	26.507654571597733
16	24.79066226845978	24.149257097747327	22.44072288477262	28.619357749020267
17	27.68332910428825	23.606529645606024	20.671572358961345	28.03856889114438
18	27.040514251881927	20.395274746962137	22.38574529871155	30.17846570244439
19	28.856184273591023	22.373058163466688	22.10521864163072	26.665538921311565
20	31.509205221460995	19.53677859539316	21.8134145309989	27.140601652146945
21	27.90605881192027	20.922495700470833	21.06064450647044	30.110800981138457
22	29.253714511263357	22.4097098875074	20.268403394513516	28.068172206715726
23	29.448250585017906	20.991570103470636	21.34821957202064	28.211959739490823
24	28.484028306408415	20.166906312554627	22.303983760466885	29.045081620570073
25	29.019707350080353	21.638614000958583	21.967069835631115	27.37460881332995
26	29.208604697059403	22.047421692181906	21.455355380755027	27.288618230003664
27	27.041923933575802	21.179057768755815	24.586258422848122	27.192759874820265
28	26.480870619414137	23.292170627872228	20.675801404042968	29.55115734867067
29	27.996278440328176	23.578336011728553	21.126899546082495	27.298486001860777
30	27.45355098818687	20.915447292001467	23.71789449942203	27.913107220389637
31	28.663057881530353	21.65975922636669	22.2927063069159	27.384476585187066
32	25.91699794186473	21.411655248244944	22.563365192139614	30.107981617750713
33	26.289153909047336	21.14099636302123	24.35366094335899	28.216188784572445
34	26.540077250556827	21.375003524204235	23.67842341199357	28.40649581324537
35	25.588542107192197	23.53745524260622	24.51013561137895	26.363867038822637
36	26.957343031943388	20.935182835715697	24.883701260255435	27.223772872085483
37	27.09126279286137	21.16496095181708	24.522822746623813	27.220953508697736
38	25.66889396374299	20.13307395190166	26.031182159068482	28.16684992528687
39	25.10220192280583	18.867179790803238	27.477515576982718	28.55310270940822
40	24.732865319010966	20.083735092616088	28.626406157489637	26.556993430883306
41	22.8142885336491	19.29290366235304	29.47926358228312	28.413544221714737
42	20.65747554202261	20.0724576390651	31.252643153176013	28.017423665736274
43	19.502946234740197	21.394739067918465	30.20383997293411	28.89847472440723
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.0
4	1.0
5	1.0
6	3.0
7	5.0
8	3.5
9	2.0
10	4.0
11	6.0
12	6.0
13	10.5
14	15.0
15	30.0
16	45.0
17	38.0
18	31.0
19	31.0
20	42.5
21	54.0
22	48.5
23	43.0
24	50.0
25	57.0
26	57.0
27	73.5
28	90.0
29	101.0
30	112.0
31	152.5
32	193.0
33	193.0
34	319.0
35	445.0
36	446.0
37	447.0
38	690.0
39	933.0
40	933.0
41	1196.5
42	1460.0
43	1899.0
44	2338.0
45	3908.5
46	5479.0
47	5479.0
48	6345.0
49	7211.0
50	8106.5
51	9002.0
52	9139.5
53	9277.0
54	9277.0
55	8556.5
56	7836.0
57	7281.5
58	6727.0
59	6316.0
60	5905.0
61	5905.0
62	5405.5
63	4906.0
64	3898.0
65	2890.0
66	2473.0
67	2056.0
68	2056.0
69	1724.0
70	1392.0
71	1143.0
72	894.0
73	679.0
74	464.0
75	464.0
76	363.5
77	263.0
78	202.5
79	142.0
80	125.5
81	109.0
82	109.0
83	84.5
84	60.0
85	41.0
86	22.0
87	16.5
88	11.0
89	11.0
90	10.0
91	9.0
92	6.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	70938.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.88085370323381
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.07927371670766	45.841439003073106
2	6.619224530375177	7.132989371000028
3	2.3939092669143425	3.869576249682822
4	1.3290774946365966	2.8644732019509993
5	0.860760818376851	2.318926386421946
6	0.6619224530375177	2.1398968113000087
7	0.40814190780178955	1.5393724097098875
8	0.33750196222071055	1.4547915080774763
9	0.27471089948197375	1.3321492007104796
>10	1.7895452880540004	18.958809100905015
>50	0.17529171681230704	6.601539372409709
>100	0.07063994558107896	5.9460373847585215
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	327	0.4609659138966421	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	236	0.3326848797541515	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	205	0.2889847472440723	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	198	0.2791169753869576	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	195	0.2748879303053371	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	178	0.2509233415094872	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	174	0.24528461473399304	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	171	0.2410555696523725	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	169	0.23823620626462544	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	167	0.23541684287687842	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	156	0.21991034424426967	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	151	0.212861935774902	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	149	0.21004257238715499	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	148	0.20863289069328145	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	148	0.20863289069328145	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	145	0.20440384561166092	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	134	0.18889734697905214	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	132	0.1860779835913051	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	127	0.17902957512193746	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	121	0.1705714849586963	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	120	0.1691618032648228	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	119	0.16775212157094926	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	119	0.16775212157094926	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	113	0.15929403140770815	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	111	0.1564746680199611	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	103	0.1451972144689729	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	102	0.14378753277509937	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	99	0.1395584876934788	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	99	0.1395584876934788	RNA PCR Primer, Index 17 (95% over 21bp)
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	98	0.1381488059996053	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	98	0.1381488059996053	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	97	0.13673912430573176	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	95	0.13391976091798471	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	94	0.1325100792241112	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	94	0.1325100792241112	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	93	0.13110039753023767	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	89	0.1254616707547436	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	88	0.12405198906087006	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	87	0.12264230736699652	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	86	0.12123262567312301	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	84	0.11841326228537595	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	83	0.11700358059150244	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	82	0.11559389889762892	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	79	0.11136485381600834	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	78	0.10995517212213483	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	78	0.10995517212213483	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	77	0.1085454904282613	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	74	0.10431644534664072	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	73	0.1029067636527672	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	73	0.1029067636527672	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	72	0.10149708195889369	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	71	0.10008740026502015	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	71	0.10008740026502015	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0014096816938735234	0.0
18	0.0	0.0	0.0	0.002819363387747047	0.0
19	0.0	0.0	0.0	0.00422904508162057	0.0
20	0.0	0.0	0.0	0.007048408469367617	0.0
21	0.0	0.0	0.0	0.009867771857114663	0.0
22	0.0	0.0	0.0	0.018325862020355802	0.0
23	0.0	0.0	0.0	0.0239645887958499	0.0
24	0.0	0.0	0.0	0.031012997265217515	0.0
25	0.0	0.0	0.0	0.03524204234683809	0.0
26	0.0	0.0	0.0	0.04651949589782627	0.0
27	0.0	0.0	0.0	0.09867771857114664	0.0
28	0.0	0.0	0.0	0.23964588795849898	0.0
29	0.0	0.0	0.0	0.46660464067213625	0.0
30	0.0	0.0	0.0	0.7894217485691731	0.0
31	0.0	0.0	0.0	1.537962728016014	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	105	0.0	29.95238	1
TGGTCAG	25	0.0054620584	29.599998	30
GATAAAC	25	0.0054620584	29.599998	3
ATAAACT	25	0.0054620584	29.599998	4
GGTAGAG	25	0.0054620584	29.599998	36
AGGTAGA	25	0.0054620584	29.599998	35
GTCAGGT	25	0.0054620584	29.599998	32
AAATCGT	35	8.787237E-4	26.42857	5
GACACAG	35	8.787237E-4	26.42857	22
CGTACCC	35	8.787237E-4	26.42857	9
ACCCCAA	35	8.787237E-4	26.42857	12
TACCCCA	35	8.787237E-4	26.42857	11
CAAATCG	35	8.787237E-4	26.42857	4
CCGACAC	35	8.787237E-4	26.42857	20
CCAAACC	45	1.3061856E-4	24.666666	15
CCCAAAC	45	1.3061856E-4	24.666666	14
CCCCAAA	45	1.3061856E-4	24.666666	13
AACCGAC	40	0.0019134671	23.125002	18
TCAAATC	40	0.0019134671	23.125002	3
TCGTACC	40	0.0019134671	23.125002	8
>>END_MODULE