FastQCFastQC Report
Fri 10 Feb 2017
ERR1632610.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632610.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569397
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9990.17544876421898956No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7830.13751389627974858No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7730.1357576523936726No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7220.12680080857468515No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6190.10871149654810264No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6180.10853587215949505No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5990.10519900877595069No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5860.10291589172405192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC200.001841156337.03
GGTATCA5300.032.4622651
TATACCA509.0805715E-625.8999985
GTCTAAA400.001929997123.1251
ATACGGC1005.329639E-1022.19999929
AACATGC450.00382347220.5555577
TTTTACG450.00382347220.5555574
CGTATGC555.1387126E-420.18181831
CGTAGAC555.1387126E-420.1818183
TCGTATG555.1387126E-420.18181830
TAATACG3950.020.1392427
GTATCAA8550.020.1228072
TCTGTAC656.894315E-519.9230773
GTAACGT2600.019.92307726
CGGGTAA2700.019.8703723
GCGGTAA4200.019.82142823
GGTAACG2750.019.50909225
GGTAATA4350.019.1379325
CGCGGTA4500.018.91111222
CGTGCCA4800.018.88541610
Produced by FastQC (version 0.11.5)