##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632610.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	569397
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07346543799844	34.0	33.0	34.0	31.0	34.0
2	33.21024171184604	34.0	33.0	34.0	31.0	34.0
3	33.28491895812588	34.0	34.0	34.0	31.0	34.0
4	36.563942205526196	37.0	37.0	37.0	35.0	37.0
5	36.53255637103813	37.0	37.0	37.0	35.0	37.0
6	36.57454289362255	37.0	37.0	37.0	35.0	37.0
7	36.57073008814588	37.0	37.0	37.0	35.0	37.0
8	36.542415924214566	37.0	37.0	37.0	35.0	37.0
9	38.33645593496278	39.0	39.0	39.0	37.0	39.0
10	38.37014069269771	39.0	39.0	39.0	37.0	39.0
11	38.436391480812155	39.0	39.0	39.0	37.0	39.0
12	38.40591713690097	39.0	39.0	39.0	37.0	39.0
13	38.4342295445884	39.0	39.0	39.0	37.0	39.0
14	40.00926067401128	41.0	40.0	41.0	38.0	41.0
15	40.00477522712624	41.0	40.0	41.0	38.0	41.0
16	39.975049043110516	41.0	40.0	41.0	38.0	41.0
17	39.96901634536185	41.0	40.0	41.0	38.0	41.0
18	39.966000874609456	41.0	40.0	41.0	38.0	41.0
19	39.966780646894875	41.0	40.0	41.0	38.0	41.0
20	39.940236776800724	41.0	40.0	41.0	38.0	41.0
21	39.90654323784635	41.0	40.0	41.0	38.0	41.0
22	39.881272644569606	41.0	40.0	41.0	38.0	41.0
23	39.838660899161745	41.0	40.0	41.0	38.0	41.0
24	39.817996933598174	41.0	40.0	41.0	38.0	41.0
25	39.773244326893185	41.0	40.0	41.0	38.0	41.0
26	39.68658247233477	41.0	40.0	41.0	38.0	41.0
27	39.61128527196315	41.0	40.0	41.0	37.0	41.0
28	39.57077926297469	41.0	40.0	41.0	37.0	41.0
29	39.529600612577866	41.0	40.0	41.0	37.0	41.0
30	39.48482693094625	41.0	40.0	41.0	37.0	41.0
31	39.42654246509905	41.0	39.0	41.0	37.0	41.0
32	39.3714666568317	41.0	39.0	41.0	36.0	41.0
33	39.31091839261535	41.0	39.0	41.0	36.0	41.0
34	39.26536142621053	41.0	39.0	41.0	36.0	41.0
35	39.1831920435127	41.0	39.0	41.0	35.0	41.0
36	39.13428065128548	41.0	39.0	41.0	35.0	41.0
37	39.08022522071595	41.0	39.0	41.0	35.0	41.0
38	39.001752731398305	41.0	39.0	41.0	35.0	41.0
39	38.919462167872325	41.0	39.0	41.0	35.0	41.0
40	38.8473191815201	40.0	39.0	41.0	35.0	41.0
41	38.79245412251909	40.0	38.0	41.0	35.0	41.0
42	38.73212538878849	40.0	38.0	41.0	35.0	41.0
43	38.00248859758657	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	1.0
16	1.0
17	6.0
18	15.0
19	25.0
20	42.0
21	60.0
22	130.0
23	210.0
24	324.0
25	492.0
26	703.0
27	960.0
28	1303.0
29	1712.0
30	2310.0
31	3069.0
32	3992.0
33	5436.0
34	8268.0
35	13406.0
36	21418.0
37	41153.0
38	108033.0
39	356327.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.357270937500545	16.07911527457995	12.344462650839397	28.219151137080107
2	21.27215282131799	19.078428583220493	32.051977794052306	27.597440801409213
3	21.071589769528114	19.471300340535688	28.238294195438336	31.218815694497863
4	16.08859899156476	15.343249086314117	32.77871151411054	35.789440408010584
5	17.458820471481236	32.710218002553574	32.91095667873206	16.920004847233123
6	35.90412313377134	32.81840262593586	14.763864228297654	16.513610011995144
7	31.721628319081415	26.983984812002877	19.26722480097366	22.02716206794205
8	28.062142933664912	30.767987888942162	19.631821031723035	21.538048145669894
9	28.364216882069982	12.737334408154592	16.908238013196417	41.99021069657901
10	19.641304748707846	23.306761363337003	29.440794384234547	27.611139503720604
11	39.74415039067645	19.338001429582523	18.42756459904074	22.490283580700286
12	23.426537196367388	23.003282419823076	26.338565183869957	27.231615199939586
13	35.17387692594095	17.217512561534395	20.697334197405326	26.911276315119327
14	24.888434607137025	18.949871530759737	22.46481804435218	33.69687581775106
15	29.52544533954341	23.62411463355093	19.446888550519233	27.403551476386422
16	26.669617156395276	23.254952168697763	21.640085915450907	28.435344759456054
17	27.515775460706678	23.069141565550925	21.600043554848376	27.81503941889402
18	27.152584225066168	21.37366371793318	22.668542335136994	28.805209721863655
19	28.824352780221886	21.80201160174711	22.425302556915476	26.94833306111553
20	29.93113767722696	20.809909430502795	22.013638990019267	27.245313902250977
21	28.005065007367442	21.79112288965344	21.38174243980913	28.822069663169987
22	28.960286057004165	22.31676668475598	20.632001924843298	28.09094533339656
23	28.691229493657328	21.86225076703952	21.51925633608888	27.927263403214276
24	28.302221472891496	21.325191386677485	21.84152708918382	28.5310600512472
25	28.84419833613454	21.53471128228635	22.08933310150914	27.531757280069968
26	28.305733960663648	22.29779925078636	21.999062165764833	27.397404622785153
27	27.916901564286427	21.541560633442046	22.850664826123072	27.69087297614845
28	27.293259360340848	22.458846815139523	21.587047350091414	28.660846474428208
29	27.69087297614845	22.775497587799023	21.737381826739515	27.796247609313006
30	27.38282779853073	22.2686456022775	22.741777705186365	27.606748894005413
31	28.187538747130738	21.925299922549645	21.935486137088887	27.951675193230734
32	26.047379947558557	21.901941878864832	22.669420457080033	29.381257716496574
33	26.457638519345906	21.63516843256989	23.715263691238274	28.191929356845925
34	26.955006787882617	21.278475299307864	23.427766567087637	28.33875134572188
35	26.550192572142105	22.459373688305348	24.195596394080056	26.794837345472487
36	26.481523436196536	21.450060326977486	24.583023795348414	27.485392441477565
37	26.743028150833247	20.86206987391925	24.474487923188917	27.92041405205858
38	25.518574913461084	20.44724506802811	25.913554163439567	28.12062585507124
39	25.060019634806647	19.572635612762273	26.952021173276293	28.415323579154787
40	24.624295526671197	20.22771458226861	28.077246631085167	27.07074325997503
41	23.232296622567382	19.57930933952936	29.025442705177586	28.162951332725672
42	22.113744891525595	20.014857823276202	29.789584420009234	28.081812865188965
43	21.32852825006103	20.263893206321775	29.733560240043417	28.674018303573778
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	4.0
2	6.0
3	7.5
4	9.0
5	9.0
6	16.0
7	23.0
8	21.5
9	20.0
10	33.5
11	47.0
12	47.0
13	81.5
14	116.0
15	216.0
16	316.0
17	314.0
18	312.0
19	312.0
20	399.5
21	487.0
22	536.5
23	586.0
24	716.0
25	846.0
26	846.0
27	1100.0
28	1354.0
29	1858.0
30	2362.0
31	3137.0
32	3912.0
33	3912.0
34	4984.0
35	6056.0
36	7167.5
37	8279.0
38	10343.0
39	12407.0
40	12407.0
41	14865.0
42	17323.0
43	20437.5
44	23552.0
45	30560.0
46	37568.0
47	37568.0
48	42415.5
49	47263.0
50	52240.0
51	57217.0
52	60808.0
53	64399.0
54	64399.0
55	60945.5
56	57492.0
57	55251.5
58	53011.0
59	49336.5
60	45662.0
61	45662.0
62	42494.5
63	39327.0
64	33767.5
65	28208.0
66	24565.5
67	20923.0
68	20923.0
69	17808.5
70	14694.0
71	12607.5
72	10521.0
73	8410.0
74	6299.0
75	6299.0
76	5086.0
77	3873.0
78	3110.0
79	2347.0
80	1845.5
81	1344.0
82	1344.0
83	1002.5
84	661.0
85	486.0
86	311.0
87	234.5
88	158.0
89	158.0
90	114.0
91	70.0
92	47.0
93	24.0
94	14.5
95	5.0
96	5.0
97	4.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	569397.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.8746028326193
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.02950065583396	49.49768284521738
2	6.597922222066079	7.5050841180104255
3	2.014634554568144	3.4374462043180234
4	1.0472425373318077	2.382460135206842
5	0.6071034060096908	1.7264382547565797
6	0.41675578407848807	1.4221691818596516
7	0.30466486039091834	1.2129385052252224
8	0.22723294052751516	1.0339026594392504
9	0.18664913437075215	0.955403584774985
>10	1.345694112294866	14.89551130376653
>50	0.13548204758919302	5.371800457422076
>100	0.0827773590698571	8.987737019790835
>500	0.004340385868831447	1.5714257302121912
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	999	0.17544876421898956	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	783	0.13751389627974858	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	773	0.1357576523936726	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	722	0.12680080857468515	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	619	0.10871149654810264	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	618	0.10853587215949505	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	599	0.10519900877595069	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	586	0.10291589172405192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.7562438860759715E-4	0.0
4	0.0	0.0	0.0	1.7562438860759715E-4	0.0
5	0.0	0.0	0.0	1.7562438860759715E-4	0.0
6	0.0	0.0	0.0	1.7562438860759715E-4	1.7562438860759715E-4
7	0.0	0.0	0.0	1.7562438860759715E-4	1.7562438860759715E-4
8	3.512487772151943E-4	0.0	0.0	1.7562438860759715E-4	1.7562438860759715E-4
9	3.512487772151943E-4	0.0	0.0	1.7562438860759715E-4	1.7562438860759715E-4
10	3.512487772151943E-4	0.0	0.0	1.7562438860759715E-4	1.7562438860759715E-4
11	3.512487772151943E-4	0.0	0.0	1.7562438860759715E-4	1.7562438860759715E-4
12	3.512487772151943E-4	0.0	0.0	1.7562438860759715E-4	1.7562438860759715E-4
13	3.512487772151943E-4	0.0	0.0	1.7562438860759715E-4	1.7562438860759715E-4
14	3.512487772151943E-4	0.0	0.0	1.7562438860759715E-4	1.7562438860759715E-4
15	5.268731658227914E-4	0.0	0.0	1.7562438860759715E-4	1.7562438860759715E-4
16	5.268731658227914E-4	0.0	0.0	7.024975544303886E-4	1.7562438860759715E-4
17	5.268731658227914E-4	0.0	0.0	0.0014049951088607772	1.7562438860759715E-4
18	5.268731658227914E-4	0.0	0.0	0.0014049951088607772	1.7562438860759715E-4
19	5.268731658227914E-4	0.0	0.0	0.0014049951088607772	1.7562438860759715E-4
20	5.268731658227914E-4	0.0	0.0	0.0017562438860759715	1.7562438860759715E-4
21	5.268731658227914E-4	0.0	0.0	0.003336863383544346	1.7562438860759715E-4
22	5.268731658227914E-4	0.0	0.0	0.005093107269620318	1.7562438860759715E-4
23	5.268731658227914E-4	0.0	0.0	0.005795604824050706	1.7562438860759715E-4
24	5.268731658227914E-4	0.0	0.0	0.007551848710126678	1.7562438860759715E-4
25	5.268731658227914E-4	0.0	0.0	0.00807872187594947	1.7562438860759715E-4
26	5.268731658227914E-4	0.0	0.0	0.010713087705063427	1.7562438860759715E-4
27	7.024975544303886E-4	0.0	0.0	0.034422380167089046	1.7562438860759715E-4
28	7.024975544303886E-4	0.0	0.0	0.15209072053417913	1.7562438860759715E-4
29	7.024975544303886E-4	0.0	0.0	0.3359694554063334	1.7562438860759715E-4
30	7.024975544303886E-4	0.0	0.0	0.5653349069278553	1.7562438860759715E-4
31	7.024975544303886E-4	0.0	0.0	1.2070664229000152	1.7562438860759715E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATAC	20	0.0018411563	37.0	3
GGTATCA	530	0.0	32.462265	1
TATACCA	50	9.0805715E-6	25.899998	5
GTCTAAA	40	0.0019299971	23.125	1
ATACGGC	100	5.329639E-10	22.199999	29
AACATGC	45	0.003823472	20.555557	7
TTTTACG	45	0.003823472	20.555557	4
CGTATGC	55	5.1387126E-4	20.181818	31
CGTAGAC	55	5.1387126E-4	20.181818	3
TCGTATG	55	5.1387126E-4	20.181818	30
TAATACG	395	0.0	20.13924	27
GTATCAA	855	0.0	20.122807	2
TCTGTAC	65	6.894315E-5	19.923077	3
GTAACGT	260	0.0	19.923077	26
CGGGTAA	270	0.0	19.87037	23
GCGGTAA	420	0.0	19.821428	23
GGTAACG	275	0.0	19.509092	25
GGTAATA	435	0.0	19.13793	25
CGCGGTA	450	0.0	18.911112	22
CGTGCCA	480	0.0	18.885416	10
>>END_MODULE