FastQCFastQC Report
Fri 10 Feb 2017
ERR1632609.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632609.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58337
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3480.5965339321528361No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2750.471398940638017No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2060.35312066098702366No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1790.30683785590620016No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1780.30512367794024375No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1780.30512367794024375No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1760.3016953220083309No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1610.2759826525189845No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT1580.27084011862111523TruSeq Adapter, Index 11 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1430.24512744913176887No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1340.22969984743816102No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1320.22627149150624817No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1310.22455731354029174No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1300.2228431355743353No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1220.20912971184668394No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1210.2074155338807275No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1210.2074155338807275No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1200.20570135591477107No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1190.20398717794881463No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1190.20398717794881463No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1160.19884464405094537No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1080.18513122032329396No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1070.18341704235733752No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1030.17656033049351183No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1010.17313197456159898No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1010.17313197456159898No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC980.1679894406637297No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA970.16627526269777326No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA960.16456108473181685No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA930.1594185508339476No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC910.15599019490203475No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA900.1542760169360783No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA890.15256183897012188No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT890.15256183897012188No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA870.14913348303820903No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC850.14570512710629618No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC850.14570512710629618No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC850.14570512710629618No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA830.14227677117438334No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA820.1405625932084269No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC800.13713423727651403No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG800.13713423727651403No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC760.13027752541268836No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC760.13027752541268836No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC760.13027752541268836No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG760.13027752541268836No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA750.12856334744673192No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA720.12342081354886265No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG710.12170663558290622No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC700.1199924576169498No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG660.11313574575312409No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT650.11142156778716765No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT650.11142156778716765No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC650.11142156778716765No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG650.11142156778716765No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG640.10970738982121124No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC620.10627903388929838No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT620.10627903388929838No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGCTGTCT600.10285067795738553No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC590.10113649999142912No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT590.10113649999142912No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG590.10113649999142912No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG590.10113649999142912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCTG251.21930905E-437.036
TAACCCT200.001827643537.035
TTAACCC303.5580967E-430.83333434
ACGCTTC405.8454367E-527.7525
GCGCCTT405.8454367E-527.7513
GGTATCA1150.027.3478261
CGGAGCT556.0921593E-726.9090932
TTCCAGA451.3022876E-424.66666619
GGTAATA1300.024.19230825
CGCGGTA1400.023.78571522
TCGCGCG551.8628218E-523.5454549
CGCGCGC551.8628218E-523.54545410
TTCCACT400.001909402923.12529
GCGGTAA1450.022.96551723
CGGTAAT1400.022.46428724
CTTTCCA502.6597167E-422.217
TATCGCG502.6597167E-422.27
CGCTTCC502.6597167E-422.226
GCTAACT1500.022.22
AGACGCT502.6597167E-422.223
Produced by FastQC (version 0.11.5)