##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632609.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	58337
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.029758129489004	34.0	33.0	34.0	31.0	34.0
2	33.16624097913846	34.0	33.0	34.0	31.0	34.0
3	33.229562713200885	34.0	33.0	34.0	31.0	34.0
4	36.51471621783774	37.0	37.0	37.0	35.0	37.0
5	36.48698081834856	37.0	37.0	37.0	35.0	37.0
6	36.545777122580866	37.0	37.0	37.0	35.0	37.0
7	36.56561016164698	37.0	37.0	37.0	35.0	37.0
8	36.522378593345564	37.0	37.0	37.0	35.0	37.0
9	38.330613504294014	39.0	39.0	39.0	37.0	39.0
10	38.36947391878225	39.0	39.0	39.0	37.0	39.0
11	38.423984778099666	39.0	39.0	39.0	37.0	39.0
12	38.36299432607093	39.0	39.0	39.0	37.0	39.0
13	38.407374393609544	39.0	39.0	39.0	37.0	39.0
14	39.92757598093834	41.0	40.0	41.0	38.0	41.0
15	39.94190650873374	41.0	40.0	41.0	38.0	41.0
16	39.8723965922142	41.0	40.0	41.0	38.0	41.0
17	39.90287467644891	41.0	40.0	41.0	38.0	41.0
18	39.91345115449886	41.0	40.0	41.0	38.0	41.0
19	39.895126592042786	41.0	40.0	41.0	38.0	41.0
20	39.88372730856918	41.0	40.0	41.0	38.0	41.0
21	39.84395837975899	41.0	40.0	41.0	38.0	41.0
22	39.814388809846236	41.0	40.0	41.0	38.0	41.0
23	39.779111027306854	41.0	40.0	41.0	38.0	41.0
24	39.74206764146253	41.0	40.0	41.0	38.0	41.0
25	39.696470507568094	41.0	40.0	41.0	38.0	41.0
26	39.59764814783071	41.0	40.0	41.0	37.0	41.0
27	39.45033169343641	41.0	39.0	41.0	37.0	41.0
28	39.42818451411625	41.0	39.0	41.0	37.0	41.0
29	39.37573066835799	41.0	39.0	41.0	36.0	41.0
30	39.3714452234431	41.0	39.0	41.0	36.0	41.0
31	39.28366216980647	41.0	39.0	41.0	36.0	41.0
32	39.23275108421756	41.0	39.0	41.0	35.0	41.0
33	39.142362480072684	41.0	39.0	41.0	35.0	41.0
34	39.10819891321117	41.0	39.0	41.0	35.0	41.0
35	39.00005142533898	40.0	39.0	41.0	35.0	41.0
36	38.951077360851606	40.0	39.0	41.0	35.0	41.0
37	38.90589162966899	40.0	39.0	41.0	35.0	41.0
38	38.81142328196513	40.0	38.0	41.0	35.0	41.0
39	38.657181548588376	40.0	38.0	41.0	35.0	41.0
40	38.57860363062893	40.0	38.0	41.0	35.0	41.0
41	38.508476610041654	40.0	38.0	41.0	35.0	41.0
42	38.430687213946555	40.0	37.0	41.0	35.0	41.0
43	37.67502614121398	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	8.0
22	11.0
23	18.0
24	36.0
25	39.0
26	66.0
27	108.0
28	136.0
29	207.0
30	266.0
31	344.0
32	482.0
33	656.0
34	915.0
35	1554.0
36	2376.0
37	4738.0
38	12309.0
39	34063.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.13043180142962	15.6727291427396	12.870048168400844	28.32679088742993
2	23.211683837015958	19.45763409157139	29.951831599156627	27.378850472256026
3	21.732348252395564	18.771962905188815	29.281588014467662	30.21410082794796
4	15.437886761403568	16.404683134202994	35.66347258172343	32.49395752267
5	17.196633354474862	32.22825993794676	32.57623806503591	17.99886864254247
6	31.105473370245303	34.96751632754513	16.217837735913744	17.709172566295834
7	28.885612904331726	27.209146853626343	18.777105439086686	25.128134802955245
8	26.934878379073318	29.01931878567633	21.219809040574592	22.825993794675764
9	28.199941717949155	11.808972007473816	16.413254024032774	43.577832250544255
10	20.91297118466839	21.171812057527813	26.636611412996896	31.2786053448069
11	41.19683905583077	18.514836210295353	17.446903337504498	22.84142139636937
12	22.471158955722785	23.348818074292474	26.052076726605755	28.127946243378986
13	39.045545708555466	16.418396557930645	18.29542143065293	26.240636302860963
14	23.97277885390061	20.181017193204998	22.49687162521213	33.349332327682255
15	31.643725251555615	22.75571249807155	18.417128066235836	27.183434184136996
16	24.773299964002263	23.77222003188371	22.394020947254745	29.060459056859283
17	28.463925124706446	23.117404048888353	20.0747381593157	28.3439326670895
18	27.80910914171109	19.046231379741847	21.78548776934021	31.35917170920685
19	29.57985498054408	21.40322608293193	22.17117781168041	26.84574112484358
20	32.675660387061384	18.57826079503574	20.686699693162144	28.05937912474073
21	28.688482438246737	20.15187616778374	20.7946929050174	30.36494848895212
22	29.812983183914156	21.94490632017416	19.286216294975745	28.955894200935937
23	30.229528429641565	19.71476078646485	20.672986269434492	29.382724514459092
24	28.945609133140206	19.246790201758746	22.034043574403896	29.773557090697157
25	30.03068378559062	20.796407082983357	21.492363337161663	27.68054579426436
26	30.13696281947992	21.602070726982873	20.791264549085486	27.46970190445172
27	27.13372302312426	21.17352623549377	24.59673963350875	27.09601110787322
28	27.09943946380513	22.74542743027581	20.316437252515556	29.8386958534035
29	27.694259217992013	23.866499820011313	20.666129557570667	27.77311140442601
30	27.3171400654816	20.518710252498416	23.650513396300806	28.513636285719183
31	29.44614909919948	21.464936489706364	21.3020895829405	27.786824828153662
32	26.309203421499223	20.71241236265149	22.20031883710167	30.77806537874762
33	26.85088365874145	20.54956545588563	23.952208718309134	28.647342167063783
34	27.18514836210295	21.044962888047035	23.215112192947874	28.55477655690214
35	25.909799955431374	23.400243413271166	24.368753964036546	26.321202667260913
36	27.557124980715496	20.73298249824297	24.896720777551128	26.81317174349041
37	27.80568078577918	20.5821348372388	23.83050208272623	27.781682294255788
38	26.484049574026773	19.34106998988635	25.788093319848464	28.38678711623841
39	25.709241133414473	18.969093371273807	26.537189090971424	28.784476404340296
40	25.141848226682896	19.670192159349984	28.563347446731918	26.624612167235203
41	23.360817320054167	18.85595762552068	28.853043522978556	28.930181531446596
42	20.91297118466839	19.75247270171589	31.72771997188748	27.606836141728238
43	19.40449457462674	21.058676311774686	29.938118175428976	29.598710938169603
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	3.0
6	2.5
7	2.0
8	3.0
9	4.0
10	6.5
11	9.0
12	9.0
13	13.5
14	18.0
15	33.0
16	48.0
17	48.5
18	49.0
19	49.0
20	57.0
21	65.0
22	50.0
23	35.0
24	39.0
25	43.0
26	43.0
27	50.0
28	57.0
29	64.0
30	71.0
31	75.5
32	80.0
33	80.0
34	132.0
35	184.0
36	191.5
37	199.0
38	322.0
39	445.0
40	445.0
41	696.5
42	948.0
43	1283.5
44	1619.0
45	2797.0
46	3975.0
47	3975.0
48	4731.0
49	5487.0
50	6339.5
51	7192.0
52	7403.0
53	7614.0
54	7614.0
55	7093.0
56	6572.0
57	6247.5
58	5923.0
59	5640.5
60	5358.0
61	5358.0
62	4921.5
63	4485.0
64	3579.5
65	2674.0
66	2270.5
67	1867.0
68	1867.0
69	1590.5
70	1314.0
71	1056.5
72	799.0
73	636.0
74	473.0
75	473.0
76	381.0
77	289.0
78	236.5
79	184.0
80	149.0
81	114.0
82	114.0
83	91.5
84	69.0
85	49.5
86	30.0
87	27.0
88	24.0
89	24.0
90	16.5
91	9.0
92	7.5
93	6.0
94	3.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	58337.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.29055316522961
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.91635421637467	41.69909319985601
2	7.284068443656691	7.326396626497764
3	2.8154611766309903	4.247732999640022
4	1.6122435067148408	3.2432247115895576
5	0.9441679732769788	2.3741364828496496
6	0.6748926307178403	2.036443423556234
7	0.5453677823982549	1.9198793218711967
8	0.43288567727861477	1.7416048134117283
9	0.33744631535892017	1.5273325676671752
>10	2.1576112891130954	20.643845244013235
>50	0.1908787238393892	6.577300855374806
>100	0.08862226463971641	6.663009753672626
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	348	0.5965339321528361	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	275	0.471398940638017	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	206	0.35312066098702366	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	179	0.30683785590620016	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	178	0.30512367794024375	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	178	0.30512367794024375	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	176	0.3016953220083309	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	161	0.2759826525189845	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	158	0.27084011862111523	TruSeq Adapter, Index 11 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	143	0.24512744913176887	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	134	0.22969984743816102	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	132	0.22627149150624817	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	131	0.22455731354029174	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	130	0.2228431355743353	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	122	0.20912971184668394	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	121	0.2074155338807275	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	121	0.2074155338807275	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	120	0.20570135591477107	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	119	0.20398717794881463	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	119	0.20398717794881463	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	116	0.19884464405094537	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	108	0.18513122032329396	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	107	0.18341704235733752	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	103	0.17656033049351183	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	101	0.17313197456159898	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	101	0.17313197456159898	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	98	0.1679894406637297	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	97	0.16627526269777326	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	96	0.16456108473181685	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	93	0.1594185508339476	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	91	0.15599019490203475	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	90	0.1542760169360783	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	89	0.15256183897012188	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	89	0.15256183897012188	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	87	0.14913348303820903	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC	85	0.14570512710629618	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	85	0.14570512710629618	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	85	0.14570512710629618	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	83	0.14227677117438334	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	82	0.1405625932084269	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	80	0.13713423727651403	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	80	0.13713423727651403	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	76	0.13027752541268836	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	76	0.13027752541268836	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	76	0.13027752541268836	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	76	0.13027752541268836	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	75	0.12856334744673192	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	72	0.12342081354886265	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	71	0.12170663558290622	No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC	70	0.1199924576169498	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	66	0.11313574575312409	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	65	0.11142156778716765	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	65	0.11142156778716765	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	65	0.11142156778716765	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	65	0.11142156778716765	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	64	0.10970738982121124	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	62	0.10627903388929838	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	62	0.10627903388929838	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGCTGTCT	60	0.10285067795738553	No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC	59	0.10113649999142912	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT	59	0.10113649999142912	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	59	0.10113649999142912	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	59	0.10113649999142912	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0034283559319128513	0.0
17	0.0	0.0	0.0	0.005142533897869277	0.0
18	0.0	0.0	0.0	0.005142533897869277	0.0
19	0.0	0.0	0.0	0.005142533897869277	0.0
20	0.0	0.0	0.0	0.0068567118638257025	0.0
21	0.0	0.0	0.0	0.008570889829782128	0.0
22	0.0	0.0	0.0	0.011999245761694978	0.0
23	0.0	0.0	0.0	0.013713423727651405	0.0
24	0.0	0.0	0.0	0.017141779659564257	0.0
25	0.0	0.0	0.0	0.020570135591477107	0.0
26	0.0	0.0	0.0	0.02742684745530281	0.0
27	0.0	0.0	0.0	0.0668529406723006	0.0
28	0.0	0.0	0.0	0.23655655930198674	0.0
29	0.0	0.0	0.0	0.45425716097845276	0.0
30	0.0	0.0	0.0	0.7422390592591323	0.0
31	0.0	0.0	0.0	1.4107684659821382	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCCTG	25	1.21930905E-4	37.0	36
TAACCCT	20	0.0018276435	37.0	35
TTAACCC	30	3.5580967E-4	30.833334	34
ACGCTTC	40	5.8454367E-5	27.75	25
GCGCCTT	40	5.8454367E-5	27.75	13
GGTATCA	115	0.0	27.347826	1
CGGAGCT	55	6.0921593E-7	26.90909	32
TTCCAGA	45	1.3022876E-4	24.666666	19
GGTAATA	130	0.0	24.192308	25
CGCGGTA	140	0.0	23.785715	22
TCGCGCG	55	1.8628218E-5	23.545454	9
CGCGCGC	55	1.8628218E-5	23.545454	10
TTCCACT	40	0.0019094029	23.125	29
GCGGTAA	145	0.0	22.965517	23
CGGTAAT	140	0.0	22.464287	24
CTTTCCA	50	2.6597167E-4	22.2	17
TATCGCG	50	2.6597167E-4	22.2	7
CGCTTCC	50	2.6597167E-4	22.2	26
GCTAACT	150	0.0	22.2	2
AGACGCT	50	2.6597167E-4	22.2	23
>>END_MODULE