FastQCFastQC Report
Fri 10 Feb 2017
ERR1632608.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632608.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584161
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA14720.25198532596321904No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11240.1924127081403928No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9360.16022979966139472No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA8590.14704850203967743No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7790.13335364736776334No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7600.13010111938318375No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT7400.12667740571520522No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7270.1244519918310192No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT7180.12291132068042886No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6960.11914523564565249No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6950.11897404996225355No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6890.11794693586185999No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6820.11674863607806753No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6790.11623507902787074No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC6710.11486559356067934No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6490.11109950852590297No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6460.11058595147570618No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6080.10408089550654699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGT250.005494158729.66
GGTATCA6700.026.5074631
TACGGCT851.9717845E-923.94117730
ATACGGC851.9717845E-923.94117729
GACACCG400.001930056823.1257
CCGCTTA400.001930056823.12525
GTTAAGA400.001930056823.1251
CTTATAC7750.022.237
TGTTACG502.6998986E-422.216
GTATCAA8700.020.6264382
CGTTATT450.0038235920.55555510
TATACTA450.0038235920.5555555
GTATTGC555.138933E-420.1818181
AGAGTTT656.894705E-519.9230776
TCTTATA15300.018.7418337
GCGGTAA6000.018.523
TTAGCAT609.229404E-418.54
TAGAACA701.21789555E-418.54
TAGAAAC701.21789555E-418.53
GTATATC500.007030842418.51
Produced by FastQC (version 0.11.5)