##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632608.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	584161
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07688633784179	34.0	33.0	34.0	31.0	34.0
2	33.22090314142848	34.0	33.0	34.0	31.0	34.0
3	33.29912815131445	34.0	34.0	34.0	31.0	34.0
4	36.569628920794095	37.0	37.0	37.0	35.0	37.0
5	36.53630762752049	37.0	37.0	37.0	35.0	37.0
6	36.58079365106538	37.0	37.0	37.0	35.0	37.0
7	36.578070086842494	37.0	37.0	37.0	35.0	37.0
8	36.55580054128913	37.0	37.0	37.0	35.0	37.0
9	38.349232488988484	39.0	39.0	39.0	37.0	39.0
10	38.38562485342226	39.0	39.0	39.0	37.0	39.0
11	38.44910050482658	39.0	39.0	39.0	37.0	39.0
12	38.41862260575424	39.0	39.0	39.0	37.0	39.0
13	38.44512386140122	39.0	39.0	39.0	37.0	39.0
14	40.016086318669	41.0	40.0	41.0	38.0	41.0
15	40.01188713385522	41.0	40.0	41.0	38.0	41.0
16	39.974570366731086	41.0	40.0	41.0	38.0	41.0
17	39.97629420656292	41.0	40.0	41.0	38.0	41.0
18	39.973808590439965	41.0	40.0	41.0	38.0	41.0
19	39.97696011887134	41.0	40.0	41.0	38.0	41.0
20	39.95127028336366	41.0	40.0	41.0	38.0	41.0
21	39.92186743038306	41.0	40.0	41.0	38.0	41.0
22	39.896893151031996	41.0	40.0	41.0	38.0	41.0
23	39.8539323919262	41.0	40.0	41.0	38.0	41.0
24	39.838073750216125	41.0	40.0	41.0	38.0	41.0
25	39.80691453212385	41.0	40.0	41.0	38.0	41.0
26	39.72441330386657	41.0	40.0	41.0	38.0	41.0
27	39.6413334679994	41.0	40.0	41.0	37.0	41.0
28	39.6033833138467	41.0	40.0	41.0	37.0	41.0
29	39.569315650993474	41.0	40.0	41.0	37.0	41.0
30	39.5290099818372	41.0	40.0	41.0	37.0	41.0
31	39.46996632777608	41.0	40.0	41.0	37.0	41.0
32	39.41893416369802	41.0	39.0	41.0	36.0	41.0
33	39.35975527294701	41.0	39.0	41.0	36.0	41.0
34	39.32652813179928	41.0	39.0	41.0	36.0	41.0
35	39.24501464493522	41.0	39.0	41.0	35.0	41.0
36	39.1992276101965	41.0	39.0	41.0	35.0	41.0
37	39.14912156066564	41.0	39.0	41.0	35.0	41.0
38	39.07497248190139	41.0	39.0	41.0	35.0	41.0
39	38.98404549430722	41.0	39.0	41.0	35.0	41.0
40	38.91235293009975	40.0	39.0	41.0	35.0	41.0
41	38.8569949038022	40.0	39.0	41.0	35.0	41.0
42	38.79829533296471	40.0	38.0	41.0	35.0	41.0
43	38.090906445312164	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	1.0
14	0.0
15	0.0
16	1.0
17	4.0
18	9.0
19	21.0
20	24.0
21	58.0
22	116.0
23	222.0
24	319.0
25	480.0
26	675.0
27	859.0
28	1276.0
29	1774.0
30	2384.0
31	3120.0
32	4040.0
33	5640.0
34	8205.0
35	13296.0
36	21286.0
37	40668.0
38	106876.0
39	372806.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.7964208497315	16.389830885663372	12.610050996215087	28.203697268390048
2	21.468738926426106	19.096618911567187	31.92407572569891	27.510566436307798
3	21.56032326704453	19.307006116464468	28.466124921040603	30.6665456954504
4	16.130484575313996	15.584402245271423	33.20762597982406	35.07748719959052
5	17.58556288420487	32.595979533039696	32.72916199472406	17.08929558803138
6	34.94909793704133	33.05270293634803	15.047050385082194	16.95114874152845
7	30.543463189086573	27.033129565308194	19.686867147926684	22.73654009767855
8	27.455787017620143	30.54329200340317	20.096856859667113	21.904064119309574
9	27.8924816959708	12.876587105267213	17.36695876650444	41.86397243225755
10	19.61394204679874	23.03782690046066	29.50402371948829	27.844207333252307
11	39.61373662397866	19.342783924294842	18.336554477275957	22.706924974450537
12	22.850720948505636	23.171180547828424	26.570928220131094	27.40717028353485
13	35.28325239103604	17.37038248017242	20.625820621369794	26.720544507421756
14	24.50591532128985	19.445152962967402	22.58675262470449	33.46217909103826
15	29.678290745188395	23.641256434441875	19.731204239927006	26.94924858044272
16	26.362766429118	23.52724676929819	22.098531055650753	28.011455745933056
17	27.175384868212703	23.192065201203093	21.60517391609505	28.027376014489157
18	26.6654227173673	21.432618747228933	23.03611504362667	28.865843491777092
19	28.50224510023778	22.077475216592685	22.72061982912245	26.699659854047088
20	30.008850299831725	20.551183663407862	22.303611504362667	27.136354532397743
21	27.824520979661425	21.808028950922775	21.551592797191184	28.81585727222461
22	28.607524295528115	22.506980096240593	20.858461965108933	28.02703364312236
23	28.569007516763357	21.880098123633722	21.882494723201308	27.66839963640161
24	28.184010914799174	21.46035082793956	22.01430769941848	28.341330557842788
25	28.730606801891945	21.7724223287758	22.39759244454868	27.099378424783577
26	28.349376284962535	22.477364973012577	21.941724969657336	27.23153377236755
27	27.62457610145148	21.5808655490524	23.504650259089534	27.289908090406584
28	26.84568124198637	22.866470031378334	21.735103849794836	28.552744876840457
29	27.37293314685506	23.312579922315937	21.839869488034978	27.474617442794024
30	27.441065048847836	22.061554948036584	23.238285335720803	27.259094667394777
31	28.065036864836916	22.145093561535262	22.16272568692535	27.627143886702466
32	25.76498602268895	21.89858617744081	22.78002126126188	29.556406538608364
33	26.512040345041864	21.64642966579419	23.90385527277583	27.937674716388116
34	26.7922713087659	21.33093445128997	23.712640864419228	28.164153375524897
35	26.184219761332923	22.527180006881665	24.409708967219654	26.878891264565762
36	26.24430593620594	21.13783700041598	25.099587271317326	27.51826979206075
37	26.621599182417178	20.604764782311726	24.879784853833105	27.893851181437995
38	25.372457250655213	20.126643168578525	26.437232201396533	28.06366737936973
39	24.84657483125371	19.326863655738745	27.525459590763507	28.301101922244037
40	24.514988162509994	20.078368805860027	28.58184644301828	26.824796588611697
41	22.726953699408213	19.554369428975914	29.495122063951545	28.223554807664325
42	21.450079686935624	20.294062766942673	30.375701219355623	27.88015632676608
43	20.623595207485607	20.738974358096485	30.085027928944246	28.552402505473662
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	3.5
2	4.0
3	13.0
4	22.0
5	22.0
6	28.5
7	35.0
8	38.5
9	42.0
10	53.5
11	65.0
12	65.0
13	137.5
14	210.0
15	358.5
16	507.0
17	498.5
18	490.0
19	490.0
20	600.5
21	711.0
22	677.0
23	643.0
24	761.0
25	879.0
26	879.0
27	1124.5
28	1370.0
29	1848.5
30	2327.0
31	3008.0
32	3689.0
33	3689.0
34	4825.0
35	5961.0
36	6759.0
37	7557.0
38	9994.5
39	12432.0
40	12432.0
41	15008.0
42	17584.0
43	20915.0
44	24246.0
45	32055.0
46	39864.0
47	39864.0
48	45446.0
49	51028.0
50	56912.5
51	62797.0
52	65826.5
53	68856.0
54	68856.0
55	64360.0
56	59864.0
57	57308.5
58	54753.0
59	51306.0
60	47859.0
61	47859.0
62	43701.0
63	39543.0
64	32917.5
65	26292.0
66	22566.0
67	18840.0
68	18840.0
69	16050.0
70	13260.0
71	11234.0
72	9208.0
73	7357.5
74	5507.0
75	5507.0
76	4393.5
77	3280.0
78	2642.0
79	2004.0
80	1580.5
81	1157.0
82	1157.0
83	901.0
84	645.0
85	499.0
86	353.0
87	257.5
88	162.0
89	162.0
90	112.0
91	62.0
92	49.0
93	36.0
94	22.0
95	8.0
96	8.0
97	6.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	584161.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.260862705241706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.61154678388593	45.26394155161282
2	6.65833009531831	6.959401499152183
3	1.995355847124959	3.128370539240963
4	1.0719289077276584	2.2407971790613943
5	0.6501345707516448	1.6988296770991478
6	0.4387616782020062	1.3758038294901893
7	0.31736788457114606	1.1610143609837964
8	0.2396874542670577	1.0021018511695683
9	0.2151446848571671	1.0119282153374614
>10	1.508294827375864	15.670066844856098
>50	0.17567358794271937	6.403215323112339
>100	0.1095492591224109	10.987280621073642
>500	0.007566465344791142	2.6509283786804962
>1k	6.57953508242708E-4	0.4463201291299415
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1472	0.25198532596321904	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1124	0.1924127081403928	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	936	0.16022979966139472	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	859	0.14704850203967743	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	779	0.13335364736776334	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	760	0.13010111938318375	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	740	0.12667740571520522	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	727	0.1244519918310192	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	718	0.12291132068042886	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	696	0.11914523564565249	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	695	0.11897404996225355	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	689	0.11794693586185999	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	682	0.11674863607806753	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	679	0.11623507902787074	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	671	0.11486559356067934	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	649	0.11109950852590297	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	646	0.11058595147570618	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	608	0.10408089550654699	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.7118568339892599E-4	0.0
6	0.0	0.0	0.0	1.7118568339892599E-4	0.0
7	0.0	0.0	0.0	1.7118568339892599E-4	0.0
8	0.0	0.0	0.0	3.4237136679785197E-4	0.0
9	0.0	0.0	0.0	3.4237136679785197E-4	0.0
10	0.0	0.0	0.0	3.4237136679785197E-4	0.0
11	0.0	0.0	0.0	3.4237136679785197E-4	0.0
12	0.0	0.0	0.0	3.4237136679785197E-4	0.0
13	0.0	0.0	0.0	5.135570501967779E-4	0.0
14	0.0	0.0	0.0	5.135570501967779E-4	0.0
15	0.0	0.0	0.0	0.0010271141003935559	0.0
16	0.0	0.0	0.0	0.0011982997837924818	0.0
17	0.0	0.0	0.0	0.0013694854671914079	0.0
18	0.0	0.0	0.0	0.00171185683398926	0.0
19	0.0	0.0	0.0	0.0018830425173881858	0.0
20	0.0	0.0	0.0	0.0023965995675849635	0.0
21	1.7118568339892599E-4	0.0	0.0	0.0041084564015742234	0.0
22	1.7118568339892599E-4	0.0	0.0	0.00684742733595704	0.0
23	1.7118568339892599E-4	0.0	0.0	0.008216912803148447	0.0
24	1.7118568339892599E-4	0.0	0.0	0.009415212586940929	0.0
25	1.7118568339892599E-4	0.0	0.0	0.011127069420930188	0.0
26	1.7118568339892599E-4	0.0	0.0	0.015577897189302265	0.0
27	1.7118568339892599E-4	0.0	0.0	0.047931991351699274	0.0
28	1.7118568339892599E-4	0.0	0.0	0.1773483680012873	0.0
29	1.7118568339892599E-4	0.0	0.0	0.37660850347763714	0.0
30	1.7118568339892599E-4	0.0	0.0	0.6328734715258294	0.0
31	1.7118568339892599E-4	0.0	0.0	1.3374737443958087	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACAGT	25	0.0054941587	29.6	6
GGTATCA	670	0.0	26.507463	1
TACGGCT	85	1.9717845E-9	23.941177	30
ATACGGC	85	1.9717845E-9	23.941177	29
GACACCG	40	0.0019300568	23.125	7
CCGCTTA	40	0.0019300568	23.125	25
GTTAAGA	40	0.0019300568	23.125	1
CTTATAC	775	0.0	22.2	37
TGTTACG	50	2.6998986E-4	22.2	16
GTATCAA	870	0.0	20.626438	2
CGTTATT	45	0.00382359	20.555555	10
TATACTA	45	0.00382359	20.555555	5
GTATTGC	55	5.138933E-4	20.181818	1
AGAGTTT	65	6.894705E-5	19.923077	6
TCTTATA	1530	0.0	18.74183	37
GCGGTAA	600	0.0	18.5	23
TTAGCAT	60	9.229404E-4	18.5	4
TAGAACA	70	1.21789555E-4	18.5	4
TAGAAAC	70	1.21789555E-4	18.5	3
GTATATC	50	0.0070308424	18.5	1
>>END_MODULE