FastQCFastQC Report
Fri 10 Feb 2017
ERR1632607.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632607.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94202
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2290.2430946264410522No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1920.2038173287191355No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1280.1358782191460903No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1250.13269357338485382No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1170.12420118468822318No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1170.12420118468822318No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1130.11995499033990785No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT1120.11889344175282904TruSeq Adapter, Index 1 (95% over 22bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1030.10933950446911954No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1020.10827795588204071No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA990.10509331012080424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCT200.00183337237.030
ATACGGC200.00183337237.029
CTCTATG200.00183337237.01
GGTATCA705.456968E-1231.7142871
CTGAATA303.5746687E-430.83333429
GCGTAAT250.005471082429.5999981
TAGATGT358.808839E-426.4285724
CTTATAC1000.025.89999837
CGCGGTA701.8842366E-723.78571522
GCGGTAA701.8842366E-723.78571523
GTATTAG400.001918126823.1250021
TAATACG753.6659185E-722.227
GGTAATA753.6659185E-722.225
CGGTAAT753.6659185E-722.224
CACGTGT603.6797617E-521.5833347
AGCCGCG806.821647E-720.812519
ACGTGTC555.095084E-420.1818188
CGCCCTA656.8165624E-519.92307716
CCGCGGT851.220671E-619.58823621
AATACGG851.220671E-619.58823628
Produced by FastQC (version 0.11.5)