##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632607.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	94202
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.08530604445765	34.0	33.0	34.0	31.0	34.0
2	33.21819069658818	34.0	33.0	34.0	31.0	34.0
3	33.2983482303985	34.0	34.0	34.0	31.0	34.0
4	36.5609435043842	37.0	37.0	37.0	35.0	37.0
5	36.54283348548863	37.0	37.0	37.0	35.0	37.0
6	36.572270227808325	37.0	37.0	37.0	35.0	37.0
7	36.57434024755313	37.0	37.0	37.0	35.0	37.0
8	36.54453196322795	37.0	37.0	37.0	35.0	37.0
9	38.34430266873315	39.0	39.0	39.0	37.0	39.0
10	38.37209401074287	39.0	39.0	39.0	37.0	39.0
11	38.45469310630347	39.0	39.0	39.0	37.0	39.0
12	38.406934035370796	39.0	39.0	39.0	37.0	39.0
13	38.43802679348634	39.0	39.0	39.0	37.0	39.0
14	40.015180144795224	41.0	40.0	41.0	38.0	41.0
15	40.01173011188722	41.0	40.0	41.0	38.0	41.0
16	39.98500031846458	41.0	40.0	41.0	38.0	41.0
17	39.983100146493705	41.0	40.0	41.0	38.0	41.0
18	39.97988365427486	41.0	40.0	41.0	38.0	41.0
19	39.97626377359292	41.0	40.0	41.0	38.0	41.0
20	39.950818453960636	41.0	40.0	41.0	38.0	41.0
21	39.92276172480415	41.0	40.0	41.0	38.0	41.0
22	39.894630687246554	41.0	40.0	41.0	38.0	41.0
23	39.86290099997877	41.0	40.0	41.0	38.0	41.0
24	39.83985478015329	41.0	40.0	41.0	38.0	41.0
25	39.79348633786969	41.0	40.0	41.0	38.0	41.0
26	39.71997409821447	41.0	40.0	41.0	38.0	41.0
27	39.631356022165136	41.0	40.0	41.0	37.0	41.0
28	39.59943525615167	41.0	40.0	41.0	37.0	41.0
29	39.55218572854079	41.0	40.0	41.0	37.0	41.0
30	39.511751342858965	41.0	40.0	41.0	37.0	41.0
31	39.45335555508375	41.0	40.0	41.0	37.0	41.0
32	39.405118787286895	41.0	39.0	41.0	36.0	41.0
33	39.33714783125624	41.0	39.0	41.0	36.0	41.0
34	39.30301904418165	41.0	39.0	41.0	36.0	41.0
35	39.22097195388633	41.0	39.0	41.0	35.0	41.0
36	39.185654232394214	41.0	39.0	41.0	35.0	41.0
37	39.11422262796968	41.0	39.0	41.0	35.0	41.0
38	39.03089106388399	41.0	39.0	41.0	35.0	41.0
39	38.953737712575105	41.0	39.0	41.0	35.0	41.0
40	38.889248635910064	40.0	39.0	41.0	35.0	41.0
41	38.83336871828624	40.0	38.0	41.0	35.0	41.0
42	38.763327742510775	40.0	38.0	41.0	35.0	41.0
43	38.04720706566739	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	3.0
19	2.0
20	7.0
21	9.0
22	22.0
23	27.0
24	58.0
25	69.0
26	110.0
27	147.0
28	211.0
29	267.0
30	389.0
31	547.0
32	688.0
33	928.0
34	1378.0
35	2206.0
36	3424.0
37	6540.0
38	17076.0
39	60092.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.44706057196238	16.362709921233094	12.215239591516104	27.974989915288422
2	21.34986518332944	19.19810619732065	31.611855374620497	27.84017324472941
3	21.373219252245175	19.564340459862848	28.363516698159273	30.6989235897327
4	15.975244686949322	15.070805290758157	33.06299229315726	35.890957729135266
5	17.64718371159848	32.845374832806094	32.60015710919089	16.907284346404534
6	35.89732702065774	33.04282287000276	14.947665654657014	16.11218445468249
7	31.239251820555825	26.99942676376298	19.3711386170145	22.390182798666693
8	28.0110825672491	30.864525169316998	19.492155155941486	21.63223710749241
9	27.919789388760325	12.799091314409461	17.28307254623044	41.99804675059978
10	19.34353835375045	23.088681768964566	29.81996135963143	27.747818517653556
11	39.74225600305726	18.97836563979533	18.89238020424195	22.386998152905456
12	23.469777711725865	23.11840512940277	26.383728583257255	27.028088575614106
13	35.14362752383177	17.39984289080911	20.255408590051164	27.201120995307953
14	24.57272669370077	19.133351733508842	22.219273476147002	34.07464809664339
15	29.30723339207236	23.757457378824228	19.726757393685908	27.208551835417506
16	26.604530689369653	23.624763805439375	21.85303921360481	27.91766629158617
17	27.129997239973676	23.136451455383114	21.643914141950276	28.089637162692938
18	26.85080996157194	21.792530944141316	22.91246470350948	28.444194390777266
19	28.06203689942889	22.11311861743912	22.80418674762744	27.020657735504557
20	29.38472643892911	20.982569372200167	22.39336744442793	27.239336744442795
21	27.646971401881064	21.948578586441904	21.81376191588289	28.590688095794142
22	28.590688095794142	22.371074924099275	21.13330927156536	27.904927708541223
23	28.624657650580666	22.03350247340821	21.74157661196153	27.600263264049595
24	28.200038215749135	21.555805609222734	21.862593150888515	28.381563024139616
25	28.635273136451456	21.95707097513853	22.372136472686353	27.03551941572366
26	28.59387274155538	22.394428993015012	21.859408505127277	27.152289760302327
27	27.1437973716057	21.941147746332348	23.49207023205452	27.422984650007432
28	27.101335428122546	22.660877688371798	21.865777796649752	28.372009086855904
29	27.67244856797096	23.507993460860703	21.894439608500882	26.92511836266746
30	27.47712362794845	21.81270036729581	23.425192671068555	27.284983333687187
31	28.157576272265985	22.100380034394174	22.15876520668351	27.583278486656333
32	26.27226598161398	22.130103394832382	22.725632152183607	28.871998471370038
33	26.648054181439885	21.483620305301372	23.860427591770875	28.007897921487867
34	26.809409566675868	21.65346807923399	23.430500414003948	28.106621940086196
35	26.411328846521304	22.752170866860578	23.89333559797032	26.943164688647798
36	26.26801978726566	21.50591282563003	24.503726035540645	27.722341351563664
37	26.693700770684277	21.13543236873952	24.51964926434683	27.651217596229376
38	25.718137619158828	20.713997579669222	25.722383813507143	27.845480987664807
39	25.212840491709304	19.912528396424705	26.73403961699327	28.14059149487272
40	24.9824844483132	20.618458206832127	27.531262605889474	26.867794738965202
41	23.65767181163882	20.100422496337657	28.195792021400816	28.046113670622702
42	22.288274134307127	20.646058470096175	29.31891042653022	27.74675696906647
43	21.590836712596335	20.86898367338273	29.172416721513343	28.367762892507592
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	4.0
4	7.0
5	7.0
6	6.5
7	6.0
8	5.5
9	5.0
10	8.5
11	12.0
12	12.0
13	19.0
14	26.0
15	60.0
16	94.0
17	88.0
18	82.0
19	82.0
20	98.5
21	115.0
22	106.5
23	98.0
24	122.0
25	146.0
26	146.0
27	179.5
28	213.0
29	313.5
30	414.0
31	517.5
32	621.0
33	621.0
34	839.5
35	1058.0
36	1268.0
37	1478.0
38	1861.5
39	2245.0
40	2245.0
41	2710.5
42	3176.0
43	3679.0
44	4182.0
45	5203.5
46	6225.0
47	6225.0
48	6994.0
49	7763.0
50	8680.0
51	9597.0
52	9809.5
53	10022.0
54	10022.0
55	9689.0
56	9356.0
57	8991.5
58	8627.0
59	8226.5
60	7826.0
61	7826.0
62	7174.0
63	6522.0
64	5516.0
65	4510.0
66	3893.0
67	3276.0
68	3276.0
69	2871.5
70	2467.0
71	2033.5
72	1600.0
73	1266.0
74	932.0
75	932.0
76	786.5
77	641.0
78	493.0
79	345.0
80	301.0
81	257.0
82	257.0
83	192.5
84	128.0
85	101.5
86	75.0
87	55.5
88	36.0
89	36.0
90	25.5
91	15.0
92	8.5
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	94202.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.48998959682385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.64436855000372	64.08674975053609
2	5.416883213304626	7.745058491327147
3	1.7358378498774962	3.7228508948854584
4	0.8939045214938006	2.556208997685824
5	0.4840745415398322	1.7303241969384937
6	0.3563738956121464	1.528629965393516
7	0.23906748830648156	1.196365257637842
8	0.2049149899769842	1.171949640135029
9	0.11582151607394757	0.7452071081293391
>10	0.8300541985299577	11.002951105072079
>50	0.0638503229638429	3.0933525827477126
>100	0.014848912317172766	1.4203520095114754
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	229	0.2430946264410522	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	192	0.2038173287191355	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	128	0.1358782191460903	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	125	0.13269357338485382	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	117	0.12420118468822318	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	117	0.12420118468822318	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	113	0.11995499033990785	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	112	0.11889344175282904	TruSeq Adapter, Index 1 (95% over 22bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	103	0.10933950446911954	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	102	0.10827795588204071	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	99	0.10509331012080424	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0010615485870788307	0.0	0.0	0.0	0.0
9	0.0010615485870788307	0.0	0.0	0.0010615485870788307	0.0
10	0.0010615485870788307	0.0	0.0	0.0010615485870788307	0.0
11	0.0010615485870788307	0.0	0.0	0.0010615485870788307	0.0
12	0.0010615485870788307	0.0	0.0	0.0010615485870788307	0.0
13	0.0010615485870788307	0.0	0.0	0.0010615485870788307	0.0
14	0.0010615485870788307	0.0	0.0	0.0010615485870788307	0.0
15	0.0010615485870788307	0.0	0.0	0.0010615485870788307	0.0
16	0.0010615485870788307	0.0	0.0	0.0010615485870788307	0.0
17	0.0010615485870788307	0.0	0.0	0.0010615485870788307	0.0
18	0.0010615485870788307	0.0	0.0	0.0010615485870788307	0.0
19	0.0010615485870788307	0.0	0.0	0.0010615485870788307	0.0
20	0.0010615485870788307	0.0	0.0	0.0021230971741576614	0.0
21	0.0010615485870788307	0.0	0.0	0.0031846457612364916	0.0
22	0.0010615485870788307	0.0	0.0	0.005307742935394153	0.0
23	0.0010615485870788307	0.0	0.0	0.007430840109551814	0.0
24	0.0010615485870788307	0.0	0.0	0.010615485870788305	0.0
25	0.0010615485870788307	0.0	0.0	0.010615485870788305	0.0
26	0.0010615485870788307	0.0	0.0	0.012738583044945967	0.0
27	0.0010615485870788307	0.0	0.0	0.027600263264049595	0.0
28	0.0010615485870788307	0.0	0.0	0.10721640729496189	0.0
29	0.0010615485870788307	0.0	0.0	0.2462792722022887	0.0
30	0.0010615485870788307	0.0	0.0	0.4479735037472665	0.0
31	0.0010615485870788307	0.0	0.0	0.9925479289187066	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGCT	20	0.001833372	37.0	30
ATACGGC	20	0.001833372	37.0	29
CTCTATG	20	0.001833372	37.0	1
GGTATCA	70	5.456968E-12	31.714287	1
CTGAATA	30	3.5746687E-4	30.833334	29
GCGTAAT	25	0.0054710824	29.599998	1
TAGATGT	35	8.808839E-4	26.428572	4
CTTATAC	100	0.0	25.899998	37
CGCGGTA	70	1.8842366E-7	23.785715	22
GCGGTAA	70	1.8842366E-7	23.785715	23
GTATTAG	40	0.0019181268	23.125002	1
TAATACG	75	3.6659185E-7	22.2	27
GGTAATA	75	3.6659185E-7	22.2	25
CGGTAAT	75	3.6659185E-7	22.2	24
CACGTGT	60	3.6797617E-5	21.583334	7
AGCCGCG	80	6.821647E-7	20.8125	19
ACGTGTC	55	5.095084E-4	20.181818	8
CGCCCTA	65	6.8165624E-5	19.923077	16
CCGCGGT	85	1.220671E-6	19.588236	21
AATACGG	85	1.220671E-6	19.588236	28
>>END_MODULE