FastQCFastQC Report
Fri 10 Feb 2017
ERR1632606.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632606.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70318
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1190.16923120680337894No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA870.12372365539406695No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA720.10239199067095196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTG451.0556323E-732.888897
CGTGCCA451.0556323E-732.8888910
AACTCCG451.0556323E-732.888895
GGTAATA502.6598536E-729.625
CTAACTC502.6598536E-729.63
GTACTTA250.00546174629.61
TCCGTGC502.6598536E-729.68
GCTAACT502.6598536E-729.62
TAACTCC502.6598536E-729.64
CGGTAAT556.122373E-726.9090924
GTGCCAG556.122373E-726.9090911
GCGGTAA508.933628E-625.90000223
TGACCAT508.933628E-625.90000210
GTAATAC601.3078225E-624.66666826
ACTCCGT601.3078225E-624.6666686
GGCTAAC601.3078225E-624.6666681
AGCCGCG551.8699357E-523.54545419
CGCGGTA551.8699357E-523.54545422
CCGCGGT551.8699357E-523.54545421
CGCGCGA400.001913306523.12500228
Produced by FastQC (version 0.11.5)