##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632606.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70318
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.08269575357661	34.0	33.0	34.0	31.0	34.0
2	33.22091071987258	34.0	33.0	34.0	31.0	34.0
3	33.299638783810686	34.0	34.0	34.0	31.0	34.0
4	36.56974032253477	37.0	37.0	37.0	35.0	37.0
5	36.531627748229475	37.0	37.0	37.0	35.0	37.0
6	36.57330982109844	37.0	37.0	37.0	35.0	37.0
7	36.56999630251145	37.0	37.0	37.0	35.0	37.0
8	36.54375835490202	37.0	37.0	37.0	35.0	37.0
9	38.33339969851247	39.0	39.0	39.0	37.0	39.0
10	38.365667396683634	39.0	39.0	39.0	37.0	39.0
11	38.43600500583066	39.0	39.0	39.0	37.0	39.0
12	38.401191729002534	39.0	39.0	39.0	37.0	39.0
13	38.42505475127279	39.0	39.0	39.0	37.0	39.0
14	40.006883017150656	41.0	40.0	41.0	38.0	41.0
15	39.994439546062175	41.0	40.0	41.0	38.0	41.0
16	39.97022099604653	41.0	40.0	41.0	38.0	41.0
17	39.96264114451492	41.0	40.0	41.0	38.0	41.0
18	39.94866179356637	41.0	40.0	41.0	38.0	41.0
19	39.95348274979379	41.0	40.0	41.0	38.0	41.0
20	39.91922409624847	41.0	40.0	41.0	38.0	41.0
21	39.89327057083535	41.0	40.0	41.0	38.0	41.0
22	39.86023493273415	41.0	40.0	41.0	38.0	41.0
23	39.8132199436844	41.0	40.0	41.0	38.0	41.0
24	39.810589038368555	41.0	40.0	41.0	38.0	41.0
25	39.77645837481157	41.0	40.0	41.0	38.0	41.0
26	39.67645553058961	41.0	40.0	41.0	37.0	41.0
27	39.614963451747776	41.0	40.0	41.0	37.0	41.0
28	39.55651469040644	41.0	40.0	41.0	37.0	41.0
29	39.49887653232458	41.0	40.0	41.0	37.0	41.0
30	39.47552547000768	41.0	40.0	41.0	37.0	41.0
31	39.409212434938425	41.0	39.0	41.0	36.0	41.0
32	39.36755880428909	41.0	39.0	41.0	36.0	41.0
33	39.30484371000313	41.0	39.0	41.0	36.0	41.0
34	39.24815836627891	41.0	39.0	41.0	35.0	41.0
35	39.18885633834864	41.0	39.0	41.0	35.0	41.0
36	39.1429505958645	41.0	39.0	41.0	35.0	41.0
37	39.08893882078557	41.0	39.0	41.0	35.0	41.0
38	39.01988111152194	41.0	39.0	41.0	35.0	41.0
39	38.92916465200944	41.0	39.0	41.0	35.0	41.0
40	38.845359651867234	40.0	38.0	41.0	35.0	41.0
41	38.79882818055121	40.0	38.0	41.0	35.0	41.0
42	38.71490941153048	40.0	38.0	41.0	35.0	41.0
43	38.00118035211468	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	4.0
20	6.0
21	5.0
22	13.0
23	27.0
24	41.0
25	60.0
26	73.0
27	140.0
28	160.0
29	206.0
30	271.0
31	372.0
32	553.0
33	679.0
34	1004.0
35	1669.0
36	2707.0
37	5095.0
38	13292.0
39	43939.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.53934981085924	16.123894308711854	12.196023777695611	28.140732102733296
2	21.62746380727552	18.724935293950338	31.270798373105034	28.376802525669103
3	21.742654796780343	19.135925367615688	27.40407861429506	31.71734122130891
4	16.2746380727552	14.734491879746296	31.548110014505532	37.44276003299297
5	18.313945220284992	32.043004636081804	32.12833129497426	17.51471884865895
6	37.624790238630226	31.499758241133136	14.246707813077734	16.628743707158907
7	32.310361500611506	26.39437981740095	19.015045934184705	22.28021274780284
8	28.742285047925144	30.407577007309648	18.95531727295998	21.894820671805228
9	28.27441053499815	12.972496373616996	16.87761312892858	41.87547996245627
10	19.627975767228875	23.04815267783498	29.636792855314432	27.68707869962172
11	40.33533376944737	18.776131289285818	18.379362325435878	22.509172615830938
12	23.991012258596662	22.214795642651954	26.421399926050228	27.37279217270116
13	34.995307033760916	16.726869364885236	20.16268949628829	28.115134105065557
14	24.990756278619983	17.978327028641313	22.18208709007651	34.84882960266219
15	29.261355556187606	23.609886515543675	19.15441281037572	27.974345117893
16	27.510736937910636	22.69404704343127	21.27051395090873	28.52470206774937
17	27.4922494951506	22.657072157911205	21.624619585312438	28.226058761625755
18	27.45100827668591	21.357262720782728	22.789328479194516	28.40240052333684
19	28.757928268722093	21.781051793281947	22.002901106402344	27.45811883159362
20	29.717853181262267	21.03302141699138	21.688614579481783	27.560510822264572
21	28.071048664637786	21.833669899598966	21.482408487158338	28.612872948604913
22	28.911516254728518	21.899087004749852	20.68744844847692	28.50194829204471
23	28.67402372081117	21.48383059813988	21.792428681134275	28.049716999914676
24	28.181973321197983	21.633152251201686	21.37432805256122	28.81054637503911
25	29.03950624306721	21.236383287351746	21.653061804943256	28.071048664637786
26	28.534656844620155	22.132313205722575	21.58195625586621	27.751073693791064
27	27.62023948348929	21.708524133223357	22.659916379874286	28.011320003413065
28	27.682812366677094	22.364117295713758	21.51653915071532	28.436531186893827
29	27.668591256861685	22.72248926306209	21.520805483659945	28.08811399641628
30	27.670013367843225	21.928951335362214	22.335675076082936	28.06536022071163
31	28.254500981256577	21.800961347023524	21.637418584146307	28.307119087573597
32	27.008731761426663	21.789584459171195	21.853579453340537	29.34810432606161
33	26.698711567450722	21.48525270912142	23.20742910776757	28.608606615660285
34	26.99166642964817	21.26198128501948	23.1078813390597	28.638470946272648
35	26.62333968542905	21.819448789783554	23.868710714184136	27.688500810603262
36	26.66884723683836	21.165277738274696	24.01518814528286	28.150686879604088
37	26.772661338490856	20.50399613185813	24.178730908160073	28.54461162149094
38	26.074404846554227	20.31485537131318	25.413123240137658	28.197616541994936
39	25.316419693392874	19.50709633379789	26.455530589607214	28.720953383202026
40	25.103103046161724	20.1769106061037	27.435365055888962	27.284621291845617
41	23.93412781933502	19.472965670240903	28.193350209050315	28.399556301373757
42	22.473619841292415	20.40160414118718	29.009641912454846	28.115134105065557
43	22.028499104070082	20.620609232344492	28.8901845900054	28.46070707358002
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.5
4	2.0
5	2.0
6	2.5
7	3.0
8	4.0
9	5.0
10	3.0
11	1.0
12	1.0
13	9.5
14	18.0
15	30.0
16	42.0
17	42.5
18	43.0
19	43.0
20	62.5
21	82.0
22	76.5
23	71.0
24	92.5
25	114.0
26	114.0
27	129.0
28	144.0
29	205.0
30	266.0
31	365.5
32	465.0
33	465.0
34	601.0
35	737.0
36	913.0
37	1089.0
38	1323.0
39	1557.0
40	1557.0
41	1852.5
42	2148.0
43	2557.0
44	2966.0
45	3649.0
46	4332.0
47	4332.0
48	4822.0
49	5312.0
50	5913.0
51	6514.0
52	6687.5
53	6861.0
54	6861.0
55	6835.5
56	6810.0
57	6705.5
58	6601.0
59	6270.5
60	5940.0
61	5940.0
62	5547.0
63	5154.0
64	4511.5
65	3869.0
66	3441.5
67	3014.0
68	3014.0
69	2611.5
70	2209.0
71	1899.5
72	1590.0
73	1269.5
74	949.0
75	949.0
76	773.0
77	597.0
78	471.0
79	345.0
80	281.5
81	218.0
82	218.0
83	168.0
84	118.0
85	96.0
86	74.0
87	57.5
88	41.0
89	41.0
90	24.5
91	8.0
92	6.5
93	5.0
94	3.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	70318.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.08307972354163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.16009541181423	74.72624363605335
2	4.625017538936438	7.500213316647231
3	1.2452644871614986	3.0290963906823287
4	0.5980777325662971	1.9397593788219234
5	0.3332397923389926	1.3510054324639496
6	0.213975024554511	1.0409852384880116
7	0.14381927879893364	0.8162917034045336
8	0.1069875122772555	0.6939901589920078
9	0.07717132033113512	0.5631559486902358
>10	0.4717973902062579	7.011007138997128
>50	0.02280061737056265	1.1590204499559145
>100	0.0017538936438894345	0.16923120680337894
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	119	0.16923120680337894	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	87	0.12372365539406695	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	72	0.10239199067095196	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0014221109815409994	0.0
20	0.0	0.0	0.0	0.0014221109815409994	0.0
21	0.0	0.0	0.0	0.0014221109815409994	0.0
22	0.0	0.0	0.0	0.004266332944622998	0.0
23	0.0	0.0	0.0	0.0056884439261639975	0.0
24	0.0	0.0	0.0	0.0056884439261639975	0.0
25	0.0	0.0	0.0	0.008532665889245996	0.0
26	0.0	0.0	0.0	0.017065331778491993	0.0
27	0.0	0.0	0.0	0.05261810631701698	0.0
28	0.0	0.0	0.0	0.15927642993259195	0.0
29	0.0	0.0	0.0	0.3057538610313149	0.0
30	0.0	0.0	0.0	0.5105378423732188	0.0
31	0.0	0.0	0.0	1.0964475667681106	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGTG	45	1.0556323E-7	32.88889	7
CGTGCCA	45	1.0556323E-7	32.88889	10
AACTCCG	45	1.0556323E-7	32.88889	5
GGTAATA	50	2.6598536E-7	29.6	25
CTAACTC	50	2.6598536E-7	29.6	3
GTACTTA	25	0.005461746	29.6	1
TCCGTGC	50	2.6598536E-7	29.6	8
GCTAACT	50	2.6598536E-7	29.6	2
TAACTCC	50	2.6598536E-7	29.6	4
CGGTAAT	55	6.122373E-7	26.90909	24
GTGCCAG	55	6.122373E-7	26.90909	11
GCGGTAA	50	8.933628E-6	25.900002	23
TGACCAT	50	8.933628E-6	25.900002	10
GTAATAC	60	1.3078225E-6	24.666668	26
ACTCCGT	60	1.3078225E-6	24.666668	6
GGCTAAC	60	1.3078225E-6	24.666668	1
AGCCGCG	55	1.8699357E-5	23.545454	19
CGCGGTA	55	1.8699357E-5	23.545454	22
CCGCGGT	55	1.8699357E-5	23.545454	21
CGCGCGA	40	0.0019133065	23.125002	28
>>END_MODULE