FastQCFastQC Report
Fri 10 Feb 2017
ERR1632605.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632605.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318937
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12920.4050956772027077No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11820.3706061071622295No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11000.3448957004047821No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9900.3104061303643039No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8900.279051975782051No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8890.27873843423622846No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8650.27121343713648777No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8650.27121343713648777No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8380.2627478153992795No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8320.26086656612434433No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8040.2520874028413135No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7990.25051969511220085No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7810.2448759472873953No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7300.22888532845044635No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC6720.21069991879273964No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6590.20662387869704676No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA6560.20568325405957918No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6470.2028613801471764No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6470.2028613801471764No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6450.20223429705553136No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6450.20223429705553136No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6410.2009801308722412No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6300.1975311738681934No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6230.1953363830474357No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6120.19188742604338788No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6080.19063325986009774No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5720.1793457642104867No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5210.1633551453735377No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5210.1633551453735377No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC5210.1633551453735377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5090.15959264682366736No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5040.1580249390945547No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5030.1577113975487322No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC4940.15488952363632943No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4920.15426244054468435No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4790.15018640044899148No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC4710.14767806808241127No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4550.1426614033492508No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4520.1417207787117832No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC4510.14140723716596068No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4510.14140723716596068No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT4440.13921244634520297No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT4400.13795828016191286TruSeq Adapter, Index 8 (95% over 22bp)
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4340.13607703088697767No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4300.13482286470368757No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4260.13356869852039743No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4230.13262807388292985No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4210.1320009907912848No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4170.13074682460799467No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4160.13043328306217217No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC4130.12949265842470456No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4120.12917911687888203No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4110.1288655753330595No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4100.12855203378723698No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG4080.12792495069559193No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4070.12761140914976937No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.12698432605812432No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC4040.1266707845123018No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4010.1257301598748342No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC3990.12510307678318916No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3990.12510307678318916No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3960.12416245214572157No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3940.12353536905407653No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3940.12353536905407653No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC3930.12322182750825399No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3910.12259474441660892No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3900.12228120287078639No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3860.12102703668749627No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3830.12008641205002869No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3820.11977287050420615No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT3700.1160103719543358No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA3690.11569683040851327No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC3670.11506974731686823No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3620.11350203958775558No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3610.11318849804193304No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3520.11036662412953029No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA3510.11005308258370775No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC3490.10942599949206269No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3450.10817183330877257No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC3440.10785829176295005No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC3400.10660412557965994No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3390.1062905840338374No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT3370.10566350094219235No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA3310.10378225166725719No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT3300.10346871012143465No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3290.1031551685756121No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT3240.10158746084649947No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3220.10096037775485439No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG3200.10033329466320935No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA3200.10033329466320935No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT200.001839942637.07
GGTATCA6100.032.147541
TAGGACC303.5936997E-430.8333324
AAAGAGG250.005490463729.629
TTAGGAC358.855291E-426.428573
ATACGGC809.731593E-1025.437529
ACATTAA451.3202854E-424.66666615
CATTAAA451.3202854E-424.66666616
AAACATT451.3202854E-424.66666613
GGACCGT551.8970755E-523.5454546
CGGGCAC400.001928145323.12518
TACGGCT903.801688E-922.6111130
ACGGTAA502.696174E-422.222
GATAAAC603.7154292E-521.5833323
GCAGTCG1201.0913936E-1121.5833329
GGGCAGT1201.0913936E-1121.5833327
GTATCAA9350.020.973262
CAGTCGG1151.3278623E-1020.91304410
GACCGTG450.003819839620.5555557
TGCCGCT450.003819839620.55555535
Produced by FastQC (version 0.11.5)