FastQCFastQC Report
Fri 10 Feb 2017
ERR1632604.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632604.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438652
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8890.2026663505466748No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7460.1700664763867485No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6790.15479240947265716No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6220.1417980540382809No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5470.12470021794041745No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5220.11900093924112964No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5070.11558137202155695No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4660.10623455495472492No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4500.10258701658718072No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4470.10190310314326619No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4450.10144716084732318No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA200.001840695637.01
TCTAAAC451.0773874E-732.888893
GGTATCA5500.029.9363631
ATTACCT250.005492684429.633
CGCACAG250.005492684429.614
CGTATGC802.716115E-823.12531
GTCTAAA400.001929294223.1252
ATGCCGT802.716115E-823.12534
TATTAGA1107.4578566E-1121.8636362
GCGGTAA3900.021.82051323
ATGTTTA855.162292E-821.76470631
CTTATAC4700.020.86170237
CTCGTAT909.444739E-820.55555529
TCGTATG909.444739E-820.55555530
TTAAACC450.003822094520.5555554
ATAACGC450.003822094520.55555535
CTCTATG909.444739E-820.5555551
CTAGCAT450.003822094520.5555554
AACTCCG4200.020.2619045
GTATCAA8200.020.0792682
Produced by FastQC (version 0.11.5)