ERR1632603.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1632603.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38013
Sequences flagged as poor quality	0
Sequence length	43
%GC	56

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	235	0.618209559887407	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	144	0.37881777286717705	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	144	0.37881777286717705	Illumina PCR Primer Index 10 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	144	0.37881777286717705	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	139	0.3656643779759556	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	125	0.3288348722805356	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	110	0.28937468760687135	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	104	0.2735906137374056	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	101	0.2656985768026728	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	98	0.2578065398679399	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	93	0.2446531449767185	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	92	0.2420224659984742	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	91	0.23939178702022992	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	90	0.23676110804198564	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	86	0.22623839212900848	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	77	0.20256228132480994	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	73	0.19203956541183279	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	72	0.18940888643358852	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	71	0.18677820745534424	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	71	0.18677820745534424	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	67	0.17625549154236708	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	66	0.1736248125641228	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	62	0.16310209665114567	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	62	0.16310209665114567	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	62	0.16310209665114567	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	61	0.16047141767290138	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	61	0.16047141767290138	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	59	0.1552100597164128	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	58	0.15257938073816854	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	58	0.15257938073816854	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	57	0.14994870175992425	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	57	0.14994870175992425	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	55	0.14468734380343568	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	55	0.14468734380343568	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	55	0.14468734380343568	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	54	0.1420566648251914	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	54	0.1420566648251914	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	54	0.1420566648251914	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC	52	0.1367953068687028	Illumina PCR Primer Index 10 (95% over 22bp)
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	52	0.1367953068687028	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	50	0.13153394891221426	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	50	0.13153394891221426	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	50	0.13153394891221426	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	49	0.12890326993396994	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	49	0.12890326993396994	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	49	0.12890326993396994	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	48	0.12627259095572566	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	47	0.12364191197748138	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	47	0.12364191197748138	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	47	0.12364191197748138	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	47	0.12364191197748138	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	46	0.1210112329992371	No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC	46	0.1210112329992371	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	46	0.1210112329992371	No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	46	0.1210112329992371	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	45	0.11838055402099282	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	45	0.11838055402099282	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	45	0.11838055402099282	No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG	44	0.11574987504274854	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	43	0.11311919606450424	No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT	43	0.11311919606450424	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	43	0.11311919606450424	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	42	0.11048851708625995	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT	42	0.11048851708625995	No Hit
TCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACGCT	41	0.10785783810801568	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	41	0.10785783810801568	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	41	0.10785783810801568	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	41	0.10785783810801568	No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA	41	0.10785783810801568	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	40	0.10522715912977139	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	40	0.10522715912977139	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	40	0.10522715912977139	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	40	0.10522715912977139	No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTG	39	0.10259648015152711	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	30	8.102315E-6	37.0	35
GCCGTCT	30	8.102315E-6	37.0	36
TATGCCG	35	2.32854E-5	31.714285	33
ATGCCGT	35	2.32854E-5	31.714285	34
GGTATCA	80	0.0	30.0625	1
GTATGCC	40	5.7926583E-5	27.75	32
AGCCCAC	45	1.2906232E-4	24.666668	7
ATCTCGT	45	1.2906232E-4	24.666668	27
CCGTCTT	45	1.2906232E-4	24.666668	37
CGCTAAT	45	1.2906232E-4	24.666668	22
GACACTC	45	1.2906232E-4	24.666668	16
TCGCTAA	45	1.2906232E-4	24.666668	21
TAATCTC	45	1.2906232E-4	24.666668	25
CGTATGC	45	1.2906232E-4	24.666668	31
TCGTATG	45	1.2906232E-4	24.666668	30
TCTCCGA	45	1.2906232E-4	24.666668	1
CTCGCTA	45	1.2906232E-4	24.666668	20
AGACACT	45	1.2906232E-4	24.666668	15
CTCGTAT	45	1.2906232E-4	24.666668	29
GTGATAT	40	0.0018972214	23.125	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content