##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632603.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38013
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.04122273958909	34.0	33.0	34.0	31.0	34.0
2	33.17154657617131	34.0	33.0	34.0	31.0	34.0
3	33.24649462026149	34.0	34.0	34.0	31.0	34.0
4	36.523741877778654	37.0	37.0	37.0	35.0	37.0
5	36.50177570831031	37.0	37.0	37.0	35.0	37.0
6	36.548496566963934	37.0	37.0	37.0	35.0	37.0
7	36.553284402704335	37.0	37.0	37.0	35.0	37.0
8	36.521400573488016	37.0	37.0	37.0	35.0	37.0
9	38.30799989477284	39.0	39.0	39.0	37.0	39.0
10	38.365217162549655	39.0	39.0	39.0	37.0	39.0
11	38.43863941283245	39.0	39.0	39.0	37.0	39.0
12	38.3943650856286	39.0	39.0	39.0	37.0	39.0
13	38.42661720990188	39.0	39.0	39.0	37.0	39.0
14	39.955015389472024	41.0	40.0	41.0	38.0	41.0
15	39.95227948333465	41.0	40.0	41.0	38.0	41.0
16	39.89553573777392	41.0	40.0	41.0	38.0	41.0
17	39.90824191723884	41.0	40.0	41.0	38.0	41.0
18	39.91955383684529	41.0	40.0	41.0	38.0	41.0
19	39.91032015363165	41.0	40.0	41.0	38.0	41.0
20	39.90868913266514	41.0	40.0	41.0	38.0	41.0
21	39.853181806224185	41.0	40.0	41.0	38.0	41.0
22	39.83239944229606	41.0	40.0	41.0	38.0	41.0
23	39.77052587272775	41.0	40.0	41.0	38.0	41.0
24	39.75347907294873	41.0	40.0	41.0	38.0	41.0
25	39.698418961934074	41.0	40.0	41.0	38.0	41.0
26	39.594244074395604	41.0	40.0	41.0	37.0	41.0
27	39.447952016415435	41.0	39.0	41.0	37.0	41.0
28	39.408518138531555	41.0	39.0	41.0	36.0	41.0
29	39.377870728435006	41.0	39.0	41.0	36.0	41.0
30	39.306079499118724	41.0	39.0	41.0	36.0	41.0
31	39.23565622287112	41.0	39.0	41.0	35.0	41.0
32	39.17375634651304	41.0	39.0	41.0	35.0	41.0
33	39.10280693446979	41.0	39.0	41.0	35.0	41.0
34	39.03793439086628	41.0	39.0	41.0	35.0	41.0
35	38.93920500881278	40.0	39.0	41.0	35.0	41.0
36	38.84652618840923	40.0	38.0	41.0	35.0	41.0
37	38.77257780233078	40.0	38.0	41.0	35.0	41.0
38	38.65422355509957	40.0	38.0	41.0	35.0	41.0
39	38.5173756346513	40.0	38.0	41.0	35.0	41.0
40	38.42030358035409	40.0	37.0	41.0	35.0	41.0
41	38.351090416436485	40.0	37.0	41.0	35.0	41.0
42	38.243863941283244	40.0	37.0	41.0	35.0	41.0
43	37.47204903585615	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	3.0
21	4.0
22	9.0
23	11.0
24	24.0
25	37.0
26	48.0
27	64.0
28	94.0
29	142.0
30	185.0
31	261.0
32	313.0
33	389.0
34	652.0
35	1017.0
36	1611.0
37	3368.0
38	7875.0
39	21904.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.76397548207192	16.08134059400731	12.443111567095468	27.711572356825297
2	23.510378028569175	19.37495067476916	29.86872911898561	27.245942177676056
3	22.842185568095125	18.580485623339385	27.672112172151632	30.905216636413858
4	16.42332886117907	16.373345960592427	33.580617157288295	33.62270802094021
5	18.76200247283824	31.8522610685818	30.826296267066528	18.559440191513428
6	32.79141346381501	33.12814037303028	15.923499855312656	18.156946307842052
7	29.363638755162707	26.317312498355825	18.504195932970298	25.81485281351117
8	27.25383421461079	28.295583089995528	20.977034172519925	23.473548522873756
9	27.180175203219953	12.072185831163024	16.418067503222584	44.32957146239445
10	21.558414226711914	20.75868781732565	26.561965643332545	31.12093231262989
11	41.12014310893642	19.017178333727934	18.193775813537474	21.668902743798174
12	22.318680451424513	22.51335069581459	25.867466393076054	29.300502459684846
13	38.38160629258412	16.007681582616474	18.943519322337096	26.667192802462313
14	24.825717517691317	19.509115302659616	22.018783047904662	33.646384131744405
15	31.97327230158104	21.78991397679741	18.614684450056558	27.622129271564994
16	25.83589824533712	22.77115723568253	21.471601820429854	29.921342698550497
17	27.790492726172626	22.529134769684056	19.96422276589588	29.716149738247445
18	27.138084339568042	19.716938941940914	21.76623786599321	31.37873885249783
19	29.995001709941338	21.792544655775657	21.053323862889012	27.159129771393996
20	31.384000210454317	18.401599452818772	20.72711966958672	29.487280667140187
21	29.04532659879515	19.919501223265726	20.903375161129087	30.13179701681004
22	29.40309893983637	21.416357561886723	19.16712703548786	30.013416462789046
23	30.805250835240578	20.111540788677555	20.166785047220685	28.91642332886118
24	28.79015073790545	19.061899876358087	21.26114750217031	30.88680188356615
25	30.92626206823981	20.78762528608634	21.0112329992371	27.274879646436744
26	30.258069607765766	21.695209533580616	20.337779180806567	27.708941677847054
27	28.750690553231788	20.706074237760767	23.678741483176807	26.864493725830634
28	27.714203035803543	22.85796964196459	20.127324862547024	29.300502459684846
29	28.508668087233314	23.77870728435009	19.837950174940154	27.874674453476445
30	27.895719885302395	21.295346328887486	23.284139636440166	27.524794149369953
31	30.08970615315813	20.577170967826795	20.661352695130613	28.67177018388446
32	26.322573856312314	20.464051771762293	21.979322863230998	31.234051508694392
33	27.035487859416513	20.577170967826795	23.313077105200854	29.074264067555838
34	26.64088601267987	21.116460158366873	22.689606187356954	29.553047641596297
35	26.748743850787886	22.68434482940047	23.52616210243864	27.040749217373005
36	27.269618288480256	20.532449425196646	24.46005313971536	27.737879146607742
37	28.261384263278348	20.26148949043748	23.23678741483177	28.240338831452398
38	26.22260805513903	19.298660984400072	25.8937731828585	28.584957777602398
39	26.43569305237682	17.907031804908847	26.630363296766895	29.02691184594744
40	25.138768316102382	19.540683450398546	28.266645621234844	27.053902612264224
41	23.74713913661116	18.28848025675427	28.479730618472626	29.484649988161944
42	21.695209533580616	19.488069870833662	30.960460894956988	27.856259700628733
43	20.332517822850075	20.748165101412674	29.276826348880647	29.6424907268566
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	1.0
7	0.0
8	2.0
9	4.0
10	5.5
11	7.0
12	7.0
13	12.5
14	18.0
15	27.0
16	36.0
17	30.5
18	25.0
19	25.0
20	29.5
21	34.0
22	24.5
23	15.0
24	12.5
25	10.0
26	10.0
27	13.0
28	16.0
29	12.5
30	9.0
31	11.0
32	13.0
33	13.0
34	69.0
35	125.0
36	90.0
37	55.0
38	153.0
39	251.0
40	251.0
41	395.5
42	540.0
43	805.0
44	1070.0
45	1752.0
46	2434.0
47	2434.0
48	2958.5
49	3483.0
50	3899.0
51	4315.0
52	4424.5
53	4534.0
54	4534.0
55	4394.0
56	4254.0
57	4164.5
58	4075.0
59	3789.0
60	3503.0
61	3503.0
62	3231.5
63	2960.0
64	2413.0
65	1866.0
66	1680.0
67	1494.0
68	1494.0
69	1336.5
70	1179.0
71	972.5
72	766.0
73	604.5
74	443.0
75	443.0
76	345.0
77	247.0
78	195.0
79	143.0
80	99.5
81	56.0
82	56.0
83	41.0
84	26.0
85	15.5
86	5.0
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	38013.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.46065819588036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.83525243578389	44.81887775234788
2	7.061312862907195	7.550048667561097
3	2.721188859364236	4.364296424907269
4	1.4122625725814388	3.020019467024439
5	0.9792343273299873	2.6175255833530633
6	0.6150969392776301	1.9730092336832137
7	0.49699832693632523	1.8598900376187093
8	0.33953351048125185	1.4521347959908453
9	0.2804842043105994	1.3495383158393182
>10	2.0618049404586163	22.258174834924894
>50	0.15254404094085228	5.458658879856891
>100	0.04428697962798937	3.277826006892379
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	235	0.618209559887407	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	144	0.37881777286717705	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	144	0.37881777286717705	Illumina PCR Primer Index 10 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	144	0.37881777286717705	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	139	0.3656643779759556	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	125	0.3288348722805356	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	110	0.28937468760687135	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	104	0.2735906137374056	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	101	0.2656985768026728	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	98	0.2578065398679399	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	93	0.2446531449767185	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	92	0.2420224659984742	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	91	0.23939178702022992	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	90	0.23676110804198564	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	86	0.22623839212900848	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	77	0.20256228132480994	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	73	0.19203956541183279	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	72	0.18940888643358852	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	71	0.18677820745534424	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	71	0.18677820745534424	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	67	0.17625549154236708	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	66	0.1736248125641228	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	62	0.16310209665114567	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	62	0.16310209665114567	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	62	0.16310209665114567	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	61	0.16047141767290138	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	61	0.16047141767290138	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	59	0.1552100597164128	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	58	0.15257938073816854	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	58	0.15257938073816854	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	57	0.14994870175992425	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	57	0.14994870175992425	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	55	0.14468734380343568	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	55	0.14468734380343568	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	55	0.14468734380343568	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	54	0.1420566648251914	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	54	0.1420566648251914	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	54	0.1420566648251914	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC	52	0.1367953068687028	Illumina PCR Primer Index 10 (95% over 22bp)
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	52	0.1367953068687028	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	50	0.13153394891221426	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	50	0.13153394891221426	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	50	0.13153394891221426	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	49	0.12890326993396994	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	49	0.12890326993396994	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	49	0.12890326993396994	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	48	0.12627259095572566	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	47	0.12364191197748138	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	47	0.12364191197748138	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	47	0.12364191197748138	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	47	0.12364191197748138	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	46	0.1210112329992371	No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC	46	0.1210112329992371	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	46	0.1210112329992371	No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	46	0.1210112329992371	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	45	0.11838055402099282	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	45	0.11838055402099282	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	45	0.11838055402099282	No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG	44	0.11574987504274854	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	43	0.11311919606450424	No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT	43	0.11311919606450424	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	43	0.11311919606450424	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	42	0.11048851708625995	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT	42	0.11048851708625995	No Hit
TCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACGCT	41	0.10785783810801568	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	41	0.10785783810801568	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	41	0.10785783810801568	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	41	0.10785783810801568	No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA	41	0.10785783810801568	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	40	0.10522715912977139	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	40	0.10522715912977139	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	40	0.10522715912977139	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	40	0.10522715912977139	No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTG	39	0.10259648015152711	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.002630678978244285	0.0
23	0.0	0.0	0.0	0.002630678978244285	0.0
24	0.0	0.0	0.0	0.002630678978244285	0.0
25	0.0	0.0	0.0	0.00526135795648857	0.0
26	0.0	0.0	0.0	0.007892036934732855	0.0
27	0.0	0.0	0.0	0.057874937521374265	0.0
28	0.0	0.0	0.0	0.2420224659984742	0.0
29	0.0	0.0	0.0	0.4998290058664141	0.0
30	0.0	0.0	0.0	0.8576013469076369	0.0
31	0.0	0.0	0.0	1.4994870175992423	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	30	8.102315E-6	37.0	35
GCCGTCT	30	8.102315E-6	37.0	36
TATGCCG	35	2.32854E-5	31.714285	33
ATGCCGT	35	2.32854E-5	31.714285	34
GGTATCA	80	0.0	30.0625	1
GTATGCC	40	5.7926583E-5	27.75	32
AGCCCAC	45	1.2906232E-4	24.666668	7
ATCTCGT	45	1.2906232E-4	24.666668	27
CCGTCTT	45	1.2906232E-4	24.666668	37
CGCTAAT	45	1.2906232E-4	24.666668	22
GACACTC	45	1.2906232E-4	24.666668	16
TCGCTAA	45	1.2906232E-4	24.666668	21
TAATCTC	45	1.2906232E-4	24.666668	25
CGTATGC	45	1.2906232E-4	24.666668	31
TCGTATG	45	1.2906232E-4	24.666668	30
TCTCCGA	45	1.2906232E-4	24.666668	1
CTCGCTA	45	1.2906232E-4	24.666668	20
AGACACT	45	1.2906232E-4	24.666668	15
CTCGTAT	45	1.2906232E-4	24.666668	29
GTGATAT	40	0.0018972214	23.125	2
>>END_MODULE